Rhof-KO 基因敲除小鼠

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产品名称

Rhof-KO 基因敲除小鼠

产品编号

S-KO-20751

品系全称

C57BL/6JCya-Rhofem1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-23912-Rhof-B6J-VA

品系状态

使用本品系发表的文献需注明: Rhof-KO 基因敲除小鼠 mice (Strain S-KO-20751) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
ras homolog family member F (in filopodia)
基因别称
Arhf,Ifld1,Rif
染色体号
Chr 5 (Mouse)
转录本 ID
NCBI: NM_175092 | Ensembl: ENSMUST00000031401
修饰方式
全身性基因敲除
靶向范围
Exon 3~4
敲除长度
~1.0 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1345629Mice homozygous for a knock-out allele exhibit macrothrombocytopenia without an effect on filopodia generation. Homozygous knockout also leads to the reduction of the number of B cells in the spleen marginal zone.
RHOF,也称为Ras同源家族成员F,是一种小GTP酶,属于Rho-GTPase家族。Rho-GTPases是一类重要的信号分子,参与调节细胞骨架重构、细胞迁移、细胞黏附、细胞极性以及基因转录等多种细胞过程。RHOF作为Rho-GTPase家族的成员,在细胞内信号传导网络中扮演着关键角色。

RHOF在多种肿瘤的发生发展中发挥重要作用。研究表明,RHOF在B细胞来源的淋巴瘤细胞和组织中表达水平较高,且在恶性淋巴瘤中,RHOF的表达水平高于良性淋巴瘤细胞和组织[1]。此外,RHOF在胰腺癌、肝细胞癌和急性髓系白血病等肿瘤中也表现出异常表达,并与其预后相关[2,3,4]。具体来说,RHOF在胰腺癌中通过调节细胞代谢状态和促进细胞迁移、侵袭和上皮-间质转化(EMT)等恶性生物学行为,在肿瘤的发生发展中发挥重要作用[3]。在急性髓系白血病中,RHOF的高表达与患者总体生存率降低相关,且在年轻患者、接受强化化疗的患者以及IDH1、NRAS和TP53基因野生型患者中,RHOF表达对总体生存率的预测价值更为显著[4]。

RHOF的表达水平还与乳腺癌的腋窝淋巴结转移相关。研究发现,RHOF启动子区域的甲基化水平在淋巴结转移阳性的乳腺癌组织中较淋巴结转移阴性的乳腺癌组织低[5]。此外,RHOF在雌激素和雌激素受体调节剂他莫昔芬刺激的乳腺癌细胞生长中也发挥重要作用[9]。

除了在肿瘤发生发展中的作用,RHOF还与心律失常的发生发展相关。研究发现,在非瓣膜性心房颤动(NVAF)患者中,白细胞中RHOF的表达水平无明显改变,但其他Rho GTPase基因的表达水平发生显著变化[6]。

除了基因表达水平的变化,RHOF的调控机制也受到关注。研究发现,miR-3656可以靶向RHOF,调节EMT过程,从而影响胰腺癌对吉西他滨的化疗敏感性[7]。此外,MYC表达特征相关的基因签名中包含RHOF,该签名可用于预测胰腺腺癌的预后和化疗耐药性[8]。

综上所述,RHOF作为一种重要的Rho-GTPase,在多种肿瘤的发生发展、心律失常以及基因表达调控中发挥重要作用。研究RHOF的功能和调控机制,有助于深入理解其在疾病发生发展中的作用,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Gouw, Launce G, Reading, N Scott, Jenson, Stephen D, Lim, Megan S, Elenitoba-Johnson, Kojo S J. . Expression of the Rho-family GTPase gene RHOF in lymphocyte subsets and malignant lymphomas. In British journal of haematology, 129, 531-3. doi:. https://pubmed.ncbi.nlm.nih.gov/15877735/
2. Su, Yongcheng, Wang, Fen, Lei, Ziyu, Xu, Beibei, Hu, Tianhui. 2023. An Integrated Multi-Omics Analysis Identifying Immune Subtypes of Pancreatic Cancer. In International journal of molecular sciences, 25, . doi:10.3390/ijms25010142. https://pubmed.ncbi.nlm.nih.gov/38203311/
3. Li, Shi, Liu, Yu, Bai, Yifeng, Wu, Mengwan, Xia, Jianling. . Ras Homolog Family Member F, Filopodia Associated Promotes Hepatocellular Carcinoma Metastasis by Altering the Metabolic Status of Cancer Cells Through RAB3D. In Hepatology (Baltimore, Md.), 73, 2361-2379. doi:10.1002/hep.31641. https://pubmed.ncbi.nlm.nih.gov/33205519/
4. Hou, Yue, Zi, Jie, Ge, Zheng. 2021. High Expression of RhoF Predicts Worse Overall Survival: A Potential Therapeutic Target for non-M3 Acute Myeloid Leukemia. In Journal of Cancer, 12, 5530-5542. doi:10.7150/jca.52648. https://pubmed.ncbi.nlm.nih.gov/34405015/
5. Luo, Jianguo, Chen, Shaojun, Chen, Jingsen, He, Fei, Wang, Enli. 2022. Identification and validation of DNA methylation markers to predict axillary lymph node metastasis of breast cancer. In PloS one, 17, e0278270. doi:10.1371/journal.pone.0278270. https://pubmed.ncbi.nlm.nih.gov/36454866/
6. Düzen, Irfan V, Yavuz, Fethi, Vuruskan, Ertan, Sucu, Murat, Demiryürek, Abdullah T. 2019. Investigation of leukocyte RHO/ROCK gene expressions in patients with non-valvular atrial fibrillation. In Experimental and therapeutic medicine, 18, 2777-2782. doi:10.3892/etm.2019.7929. https://pubmed.ncbi.nlm.nih.gov/31572525/
7. Yang, Rui-Meng, Zhan, Ming, Xu, Sun-Wang, Zhu, Jun, Wang, Jian. 2017. miR-3656 expression enhances the chemosensitivity of pancreatic cancer to gemcitabine through modulation of the RHOF/EMT axis. In Cell death & disease, 8, e3129. doi:10.1038/cddis.2017.530. https://pubmed.ncbi.nlm.nih.gov/29048402/
8. Dong, Biao, Zhang, Yueshan, Gao, Han, Liu, Jia, Li, Jiankun. 2024. Machine Learning Developed a MYC Expression Feature-Based Signature for Predicting Prognosis and Chemoresistance in Pancreatic Adenocarcinoma. In Biochemical genetics, 62, 4191-4214. doi:10.1007/s10528-023-10625-0. https://pubmed.ncbi.nlm.nih.gov/38245886/
9. Fan, Ping, Cunliffe, Heather E, Griffith, Obi L, Gray, Joe W, Jordan, V Craig. 2014. Identification of gene regulation patterns underlying both oestrogen- and tamoxifen-stimulated cell growth through global gene expression profiling in breast cancer cells. In European journal of cancer (Oxford, England : 1990), 50, 2877-86. doi:10.1016/j.ejca.2014.08.010. https://pubmed.ncbi.nlm.nih.gov/25212499/