Adgre1-KO 基因敲除小鼠

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产品名称

Adgre1-KO 基因敲除小鼠

产品编号

S-KO-20027

品系全称

C57BL/6JCya-Adgre1em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-13733-Adgre1-B6J-VC

品系状态

使用本品系发表的文献需注明: Adgre1-KO 基因敲除小鼠 mice (Strain S-KO-20027) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
adhesion G protein-coupled receptor E1
基因别称
DD7A5-7,EGF-TM7,Emr1,F4/80,Gpf480,Ly71,TM7LN3
染色体号
Chr 17 (Mouse)
转录本 ID
NCBI: NM_010130 | Ensembl: ENSMUST00000086763
修饰方式
全身性基因敲除
靶向范围
Exon 10
敲除长度
~0.8 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:106912Homozygous null mice fail to develop peripheral tolerance after inoculation with antigen because of a lack of efferent regulatory T cell development.
ADGRE1,也称为EMR1或F4/80,是一种在哺乳动物的单核细胞-巨噬细胞中表达的七次跨膜G蛋白偶联受体。其细胞外结构域包含重复的表皮生长因子(EGF)样钙结合结构域。ADGRE1在巨噬细胞分化中起着关键作用,并在炎症和免疫反应中发挥重要作用。在多种疾病中,如动脉粥样硬化、糖尿病心肌病、结直肠癌和Wilms瘤,ADGRE1的表达和功能与疾病的发生和发展密切相关。此外,ADGRE1还具有独立的染色质调控功能,影响基因表达和干细胞的多能性维持。

在动脉粥样硬化中,ADGRE1通过NF-κB/IL-6信号通路介导巨噬细胞的炎症反应,促进动脉粥样硬化斑块的形成。在糖尿病心肌病中,ADGRE1通过下调lncRNA TINCR抑制焦亡和糖尿病心肌病的发生。在结直肠癌中,ADGRE1通过m6A修饰抑制SOX4 mRNA的表达,从而抑制肿瘤的转移。此外,ADGRE1的基因多态性与中国儿童Wilms瘤的易感性降低相关。

在结肠癌中,肿瘤相关巨噬细胞(TAMs)通过激活JAK2/STAT1,3信号通路,促进EMR1的表达,进而促进肿瘤的转移和进展。在急性缺血性卒中(AIS)中,FOXP3+巨噬细胞通过增强吞噬作用,抑制神经炎症,并促进组织修复。在伤口愈合中,角质形成细胞的自噬调节伤口愈合,并促进角质形成细胞和成纤维细胞的相互作用。

此外,ADGRE1在多种疾病中发挥重要作用,如肥胖、急性肾损伤和头颈部鳞状细胞癌。在肥胖小鼠中,ADGRE1的表达与卵巢炎症和卵母细胞质量下降相关。在急性肾损伤中,巨噬细胞的自噬通过抑制TARM1的降解,抑制肾脏炎症。在头颈部鳞状细胞癌中,ADGRE1的表达与肿瘤的纯度和免疫细胞浸润程度相关,并可能影响患者的预后。

综上所述,ADGRE1是一种重要的G蛋白偶联受体,在巨噬细胞分化、炎症和免疫反应中发挥重要作用。ADGRE1在多种疾病中发挥重要作用,如动脉粥样硬化、糖尿病心肌病、结直肠癌和Wilms瘤。此外,ADGRE1还具有独立的染色质调控功能,影响基因表达和干细胞的多能性维持。ADGRE1的研究有助于深入理解巨噬细胞的功能和疾病发生机制,为疾病的治疗和预防提供新的思路和策略[1,2,3,4,5,6,7,8,9,10]。

参考文献:
1. Stepp, Mary Ann, Menko, A Sue. 2021. Immune responses to injury and their links to eye disease. In Translational research : the journal of laboratory and clinical medicine, 236, 52-71. doi:10.1016/j.trsl.2021.05.005. https://pubmed.ncbi.nlm.nih.gov/34051364/
2. Park, Hee-Seon, Song, Ji-Won, Park, Jin-Ho, Won, Young-Suk, Kwon, Hyo-Jung. 2020. TXNIP/VDUP1 attenuates steatohepatitis via autophagy and fatty acid oxidation. In Autophagy, 17, 2549-2564. doi:10.1080/15548627.2020.1834711. https://pubmed.ncbi.nlm.nih.gov/33190588/
3. Cai, Wei, Hu, Mengyan, Li, Chunyi, Lu, Yan, Lu, Zhengqi. 2022. FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation. In Autophagy, 19, 1144-1163. doi:10.1080/15548627.2022.2116833. https://pubmed.ncbi.nlm.nih.gov/36170234/
4. Qiang, Lei, Yang, Seungwon, Cui, Yan-Hong, He, Yu-Ying. 2020. Keratinocyte autophagy enables the activation of keratinocytes and fibroblastsand facilitates wound healing. In Autophagy, 17, 2128-2143. doi:10.1080/15548627.2020.1816342. https://pubmed.ncbi.nlm.nih.gov/32866426/
5. Waddell, Lindsey A, Lefevre, Lucas, Bush, Stephen J, Hope, Jayne C, Hume, David A. 2018. ADGRE1 (EMR1, F4/80) Is a Rapidly-Evolving Gene Expressed in Mammalian Monocyte-Macrophages. In Frontiers in immunology, 9, 2246. doi:10.3389/fimmu.2018.02246. https://pubmed.ncbi.nlm.nih.gov/30327653/
6. Hamann, Jörg, Aust, Gabriela, Araç, Demet, Langenhan, Tobias, Schiöth, Helgi B. . International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors. In Pharmacological reviews, 67, 338-67. doi:10.1124/pr.114.009647. https://pubmed.ncbi.nlm.nih.gov/25713288/
7. Wang, Luyao, Chen, Yurong, Wei, Jiarui, Li, Ziyi, Dai, Xiangpeng. 2022. Administration of nicotinamide mononucleotide improves oocyte quality of obese mice. In Cell proliferation, 55, e13303. doi:10.1111/cpr.13303. https://pubmed.ncbi.nlm.nih.gov/35811338/
8. Akter, Rokeya, Park, Rackhyun, Lee, Soo Kyung, Park, Junsoo, Cho, Mee-Yon. 2024. Upregulation of EMR1 (ADGRE1) by Tumor-Associated Macrophages Promotes Colon Cancer Progression by Activating the JAK2/STAT1,3 Signaling Pathway in Tumor Cells. In International journal of molecular sciences, 25, . doi:10.3390/ijms25084388. https://pubmed.ncbi.nlm.nih.gov/38673975/
9. Huang, Xiao-Rong, Ye, Lin, An, Ning, Yang, Chen, Liu, Hua-Feng. 2024. Macrophage autophagy protects against acute kidney injury by inhibiting renal inflammation through the degradation of TARM1. In Autophagy, 21, 120-140. doi:10.1080/15548627.2024.2393926. https://pubmed.ncbi.nlm.nih.gov/39193910/
10. Lu, Yilong, Jia, Zengrong. 2022. Inflammation-Related Gene Signature for Predicting the Prognosis of Head and Neck Squamous Cell Carcinoma. In International journal of general medicine, 15, 4793-4805. doi:10.2147/IJGM.S354349. https://pubmed.ncbi.nlm.nih.gov/35592543/