Ppp1r12b-KO 基因敲除小鼠

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产品名称

Ppp1r12b-KO 基因敲除小鼠

产品编号

S-KO-19327

品系全称

C57BL/6JCya-Ppp1r12bem1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-329251-Ppp1r12b-B6J-VB

品系状态

使用本品系发表的文献需注明: Ppp1r12b-KO 基因敲除小鼠 mice (Strain S-KO-19327) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
protein phosphatase 1, regulatory subunit 12B
基因别称
1810037O03Rik,9530009M10Rik,Mypt2
染色体号
Chr 1 (Mouse)
转录本 ID
NCBI: NM_001081307 | Ensembl: ENSMUST00000168381
修饰方式
全身性基因敲除
靶向范围
Exon 2
敲除长度
~1.0 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1916417Mice with loss of expression in adult cardiomyocytes display blunted cardiac hypertrophic response to pressure overload. Mice homozygous for a null allele on a C57BL/6N background display improved cardiac function.
PPP1R12B(蛋白磷酸酶1调节亚基12B)是蛋白磷酸酶1(PP1)的一个调节亚基,PP1是一种重要的丝氨酸/苏氨酸蛋白磷酸酶,参与细胞周期的调控、细胞分化和信号传导等多种生物学过程。PPP1R12B通过与其他亚基结合形成PP1复合体,发挥其调节作用。PP1复合体在细胞中广泛存在,并且具有多种功能,例如在肌肉收缩过程中,PPP1R12B与MYLK3(心脏特异性肌球蛋白轻链激酶)共同调控心肌收缩力[5]。

PPP1R12B在多种疾病中发挥重要作用,包括结直肠癌、哮喘、子宫内膜息肉和食管癌等。在结直肠癌中,miR-4463通过抑制PPP1R12B的表达,促进结直肠癌细胞增殖、迁移和抑制细胞凋亡,从而促进结直肠癌的发展[1]。在哮喘中,PPP1R12B被认为是儿童哮喘的候选基因[2]。在子宫内膜息肉中,PPP1R12B的表达下调,可能通过影响血管平滑肌收缩相关的信号通路,促进子宫内膜息肉的形成[3]。在食管癌中,ADAMTS9-AS2通过吸附miR-196b-5p,调节PPP1R12B的表达,抑制食管癌细胞的恶性进程[4]。

此外,PPP1R12B在糖尿病、肺鳞状细胞癌和腹主动脉瘤中也发挥重要作用。在糖尿病中,CXCL12和PPP1R12B的表达上调,可能参与糖尿病的发生和发展[6]。在肺鳞状细胞癌中,LINC00472过表达导致PPP1R12B等肿瘤抑制基因的表达上调,而肿瘤基因的表达下调,从而抑制肺鳞状细胞癌的发生和发展[7]。在腹主动脉瘤中,PPP1R12B的表达下调,可能与m6A修饰有关,从而促进腹主动脉瘤的形成[8]。

综上所述,PPP1R12B作为一种重要的蛋白磷酸酶1调节亚基,参与多种生物学过程和疾病的发生和发展。深入研究PPP1R12B的生物学功能和调控机制,有助于我们更好地理解相关疾病的发病机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Tan, J, Lu, T, Xu, J, Jiang, B, Zhu, Y. 2022. MicroRNA-4463 facilitates the development of colon cancer by suppression of the expression of PPP1R12B. In Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 24, 1115-1123. doi:10.1007/s12094-021-02752-0. https://pubmed.ncbi.nlm.nih.gov/35064454/
2. Freidin, Maxim B, Polonikov, Alexey V. . Validation of PPP1R12B as a candidate gene for childhood asthma in Russians. In Journal of genetics, 92, 93-6. doi:. https://pubmed.ncbi.nlm.nih.gov/23640410/
3. Chiu, Christine Shan-Chi, Yeh, Ling-Yu, Pan, Szu-Hua, Li, Sheng-Hsiang. 2024. Transcriptomic Analysis Reveals Intrinsic Abnormalities in Endometrial Polyps. In International journal of molecular sciences, 25, . doi:10.3390/ijms25052557. https://pubmed.ncbi.nlm.nih.gov/38473810/
4. Chen, Zhao, Tang, Weijian, Ye, Weiwen, Song, Lijiang, Chen, Zhoumiao. 2022. ADAMTS9-AS2 regulates PPP1R12B by adsorbing miR-196b-5p and affects cell cycle-related signaling pathways inhibiting the malignant process of esophageal cancer. In Cell cycle (Georgetown, Tex.), 21, 1710-1725. doi:10.1080/15384101.2022.2067675. https://pubmed.ncbi.nlm.nih.gov/35503407/
5. Hitsumoto, Tatsuro, Tsukamoto, Osamu, Matsuoka, Ken, Haruta, Jun-Ichi, Takashima, Seiji. 2023. Restoration of Cardiac Myosin Light Chain Kinase Ameliorates Systolic Dysfunction by Reducing Superrelaxed Myosin. In Circulation, 147, 1902-1918. doi:10.1161/CIRCULATIONAHA.122.062885. https://pubmed.ncbi.nlm.nih.gov/37128901/
6. Jia, Dongdong, Wang, Kangjie, Huang, Lin, Yao, Chen, Wu, Ridong. 2023. Revealing PPP1R12B and COL1A1 as piRNA pathway genes contributing to abdominal aortic aneurysm through integrated analysis and experimental validation. In Gene, 897, 148068. doi:10.1016/j.gene.2023.148068. https://pubmed.ncbi.nlm.nih.gov/38070790/
7. Seo, Danbi, Roh, Jungwook, Chae, Yeonsoo, Kim, Wanyeon. . Gene expression profiling after LINC00472 overexpression in an NSCLC cell line1. In Cancer biomarkers : section A of Disease markers, 32, 175-188. doi:10.3233/CBM-210242. https://pubmed.ncbi.nlm.nih.gov/34397405/
8. Xu, Qiong, Zhou, Yina, Lou, Jianfen, Lu, Yunzhu, Xu, Mengli. 2024. Construction and evaluation of a metabolic correlation diagnostic model for diabetes based on machine learning algorithms. In Environmental toxicology, 39, 4635-4648. doi:10.1002/tox.24213. https://pubmed.ncbi.nlm.nih.gov/38682583/