Tinagl1-KO 基因敲除小鼠

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产品名称

Tinagl1-KO 基因敲除小鼠

产品编号

S-KO-19151

品系全称

C57BL/6JCya-Tinagl1em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-94242-Tinagl1-B6J-VC

品系状态

使用本品系发表的文献需注明: Tinagl1-KO 基因敲除小鼠 mice (Strain S-KO-19151) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
tubulointerstitial nephritis antigen-like 1
基因别称
1110021J17Rik,AZ-1,AZ1,Arg1,Lcn7,TARP,Tinagl
染色体号
Chr 4 (Mouse)
转录本 ID
NCBI: NM_023476 | Ensembl: ENSMUST00000105998
修饰方式
全身性基因敲除
靶向范围
Exon 2~12
敲除长度
~8.6 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:2137617Female mice homozygous for a null mutation display impaired fertility and homozygous pups born to homozygous females show impaired postnatal survival.
Tinagl1,也称为Tubulointerstitial Nephritis Antigen-like 1,是一种在哺乳动物中发现的蛋白。Tinagl1是一种新型基质细胞蛋白,具有与细胞粘附和扩散相关的功能。在哺乳动物中,TINAGL1被发现存在于基膜中,可能作为整合素介导的粘附的调节因子[7]。在Drosophila中,Swim是TINAGL1的直系同源基因,Swim在Drosophila胚胎中分泌并优先与各种器官的基膜和肌肉附着位点(MAS)的特定肌腱基质结合[7]。此外,Swim在体外培养系统中被发现与Wingless(wg)结合,并作为wg信号传导的必要辅助因子[7]。

在人类中,Tinagl1在多种生物学过程中发挥作用。例如,在食管癌中,YTHDF1通过增强m6A依赖性TINAGL1的翻译来促进食管癌的进展[3]。在膀胱癌中,TINAGL1的表达与不良预后相关,并且与B细胞耗竭相关基因的预后和免疫治疗效果评估相关[4]。在肺鳞状细胞癌中,TINAGL1与淋巴结转移相关,并且与患者的总体生存率相关[8]。此外,在胃癌中,TINAGL1通过调节多种基质金属蛋白酶(MMPs)的表达来促进胃癌的生长和转移[5]。

在乳腺癌症中,Tinagl1已被证明在TNBC患者中起着关键作用。例如,Tinagl1基因治疗能够抑制TNBC的生长,并通过重塑肿瘤微环境来提高患者的预后[1]。此外,Tinagl1通过同时抑制整合素/FAK和EGFR信号传导通路来抑制TNBC的进展和转移[2]。

Tinagl1在胚胎发育和妊娠中也起着重要作用。例如,Tinagl1在植入后期的Reichert膜中表达,并且与层粘连蛋白1相互作用[9]。此外,Tinagl1在植入后期子宫内膜的蜕膜中显著表达,并且与ITGA5和ITGB1整合素结合[6]。这些结果表明,Tinagl1在胚胎发育和妊娠中发挥重要作用。

Tinagl1是一种在哺乳动物中发现的蛋白,具有与细胞粘附和扩散相关的功能。Tinagl1在多种生物学过程中发挥作用,包括癌症进展、胚胎发育和妊娠。Tinagl1在TNBC患者中起着关键作用,并且可能成为治疗TNBC的候选药物。此外,Tinagl1的研究有助于深入理解其在胚胎发育和妊娠中的生理作用,为生殖医学提供新的思路和策略。

参考文献:
1. Musetti, Sara N, Huang, Leaf. 2021. Tinagl1 Gene Therapy Suppresses Growth and Remodels the Microenvironment of Triple Negative Breast Cancer. In Molecular pharmaceutics, 18, 2032-2038. doi:10.1021/acs.molpharmaceut.1c00008. https://pubmed.ncbi.nlm.nih.gov/33877834/
2. Shen, Minhong, Jiang, Yi-Zhou, Wei, Yong, Shao, Zhi-Ming, Kang, Yibin. 2019. Tinagl1 Suppresses Triple-Negative Breast Cancer Progression and Metastasis by Simultaneously Inhibiting Integrin/FAK and EGFR Signaling. In Cancer cell, 35, 64-80.e7. doi:10.1016/j.ccell.2018.11.016. https://pubmed.ncbi.nlm.nih.gov/30612941/
3. Zhang, Lin, Cai, Enmin, Xu, Yuting, Pei, Dongsheng, Wang, Qingling. 2024. YTHDF1 facilitates esophageal cancer progression via augmenting m6A-dependent TINAGL1 translation. In Cellular signalling, 122, 111332. doi:10.1016/j.cellsig.2024.111332. https://pubmed.ncbi.nlm.nih.gov/39098703/
4. Zhou, Ranran, Zhou, Jiawei, Deng, Shikai, Wu, Jiaxu, Tan, Wanlong. 2024. Developing and experimental validating a B cell exhaustion-related gene signature to assess prognosis and immunotherapeutic response in bladder cancer. In Gene, 927, 148634. doi:10.1016/j.gene.2024.148634. https://pubmed.ncbi.nlm.nih.gov/38848880/
5. Shan, Zhi-Guo, Sun, Zhen-Wei, Zhao, Li-Qun, Zhuang, Yuan, Zhao, Yong-Liang. 2020. Upregulation of Tubulointerstitial nephritis antigen like 1 promotes gastric cancer growth and metastasis by regulating multiple matrix metallopeptidase expression. In Journal of gastroenterology and hepatology, 36, 196-203. doi:10.1111/jgh.15150. https://pubmed.ncbi.nlm.nih.gov/32537806/
6. Tajiri, Yumiko, Igarashi, Tadashi, Li, Dan, Yoshizawa, Midori, Matsumoto, Hiromichi. 2009. Tubulointerstitial nephritis antigen-like 1 is expressed in the uterus and binds with integrins in decidualized endometrium during postimplantation in mice. In Biology of reproduction, 82, 263-70. doi:10.1095/biolreprod.109.080028. https://pubmed.ncbi.nlm.nih.gov/19776386/
7. Kaltezioti, Valeria, Vakaloglou, Katerina M, Charonis, Aristidis S, Zervas, Christos G. . Evidence of Swim secretion and association with extracellular matrix in the Drosophila embryo. In The International journal of developmental biology, 66, 235-241. doi:10.1387/ijdb.210205cz. https://pubmed.ncbi.nlm.nih.gov/34881800/
8. Deng, Yufei, Liu, Lifeng, Xiao, Xia, Zhao, Yin. 2023. A four-gene-based methylation signature associated with lymph node metastasis predicts overall survival in lung squamous cell carcinoma. In Genes & genetic systems, 98, 209-219. doi:10.1266/ggs.22-00111. https://pubmed.ncbi.nlm.nih.gov/37839873/
9. Igarashi, Tadashi, Tajiri, Yumiko, Sakurai, Masahiro, Yoshizawa, Midori, Matsumoto, Hiromichi. 2009. Tubulointerstitial nephritis antigen-like 1 is expressed in extraembryonic tissues and interacts with laminin 1 in the Reichert membrane at postimplantation in the mouse. In Biology of reproduction, 81, 948-55. doi:10.1095/biolreprod.109.078162. https://pubmed.ncbi.nlm.nih.gov/19587330/