Hpcal1-KO 基因敲除小鼠

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产品名称

Hpcal1-KO 基因敲除小鼠

产品编号

S-KO-18522

品系全称

C57BL/6JCya-Hpcal1em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-53602-Hpcal1-B6J-VA

品系状态

使用本品系发表的文献需注明: Hpcal1-KO 基因敲除小鼠 mice (Strain S-KO-18522) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
hippocalcin-like 1
基因别称
NVL-3,NVP-3,Nvp3,VILIP3,Vnsl3,Vsnl3
染色体号
Chr 12 (Mouse)
转录本 ID
NCBI: NM_016677 | Ensembl: ENSMUST00000071858
修饰方式
全身性基因敲除
靶向范围
Exon 2~3
敲除长度
~1.5 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1855689Mice homozygous for a null allele exhibit increased susceptibility to diethylnitrosamine/CCl4-induced liver tumors with increased lipid biosynthesis in tumors.
Hpcal1,也称为Hippocalcin-like 1,是一种重要的神经元钙感受蛋白家族成员。Hpcal1作为一种钙离子(Ca2+)传感器,在多种生物学过程中发挥重要作用,包括细胞增殖、迁移、凋亡和信号转导等[1,2,3,4,5,6,7,8]。

Hpcal1在多种癌症中发挥重要作用,包括胶质母细胞瘤、胆管癌和胰腺癌。在胶质母细胞瘤中,Hpcal1通过激活Wnt/β-catenin信号通路,促进肿瘤细胞的增殖和生长[2]。在胆管癌中,Hpcal1的高表达与患者的预后不良相关,可能成为胆管癌患者预后评估的潜在生物标志物[4]。在胰腺癌中,Hpcal1在大多数胰腺癌细胞系中表达,可能成为胰腺癌早期诊断的潜在生物标志物[7]。

此外,Hpcal1在动脉粥样硬化和腹主动脉瘤的发病机制中也发挥重要作用。Hpcal1的基因多态性与动脉粥样硬化和腹主动脉瘤的易感性相关,可能成为这些疾病风险预测的潜在生物标志物[5]。Hpcal1还可能通过影响钙离子的调节,参与心脏疾病的发生和发展[1]。

在植物中,Hpcal1作为一种受体激酶,参与调控硝酸盐的吸收。Hpcal1通过磷酸化NRT2.1,影响其与NAR2.1和NRT2.1的相互作用,从而调节硝酸盐的吸收[9]。

综上所述,Hpcal1作为一种神经元钙感受蛋白家族成员,在多种生物学过程中发挥重要作用。Hpcal1在癌症、心血管疾病和植物硝酸盐吸收等方面具有潜在的应用价值,为疾病的治疗和预防提供了新的思路和策略。

参考文献:
1. Wu, Zenan, Chen, Huan, Ke, Shiwen, Zhu, Wei, Liu, Liangji. 2023. Identifying potential biomarkers of idiopathic pulmonary fibrosis through machine learning analysis. In Scientific reports, 13, 16559. doi:10.1038/s41598-023-43834-z. https://pubmed.ncbi.nlm.nih.gov/37783761/
2. Agha, Golareh, Mendelson, Michael M, Ward-Caviness, Cavin K, Levy, Daniel, Baccarelli, Andrea A. 2019. Blood Leukocyte DNA Methylation Predicts Risk of Future Myocardial Infarction and Coronary Heart Disease. In Circulation, 140, 645-657. doi:10.1161/CIRCULATIONAHA.118.039357. https://pubmed.ncbi.nlm.nih.gov/31424985/
3. Zhang, Dongming, Liu, Xidong, Xu, Xuebin, Shan, Baochang, Liu, Bing. 2019. HPCAL1 promotes glioblastoma proliferation via activation of Wnt/β-catenin signalling pathway. In Journal of cellular and molecular medicine, 23, 3108-3117. doi:10.1111/jcmm.14083. https://pubmed.ncbi.nlm.nih.gov/30843345/
4. Ma, Mingjian, Zeng, Guangyan, Li, Jinhui, Chen, Jiancong, Lai, Jiaming. 2022. Expressional and prognostic value of HPCAL1 in cholangiocarcinoma via integrated bioinformatics analyses and experiments. In Cancer medicine, 12, 824-836. doi:10.1002/cam4.4897. https://pubmed.ncbi.nlm.nih.gov/35645147/
5. Li, Tan, Wu, Yijun, Yang, Jun, Ma, Chunyan, Sun, Liping. 2024. N6-methyladenosine-associated genetic variants in NECTIN2 and HPCAL1 are risk factors for abdominal aortic aneurysm. In iScience, 27, 109419. doi:10.1016/j.isci.2024.109419. https://pubmed.ncbi.nlm.nih.gov/38510151/
6. Wang, Xiangyu, Xie, Xiaomeng, Zhang, Yuanyuan, Dong, Zigang, Kim, Dong Joon. 2022. Hippocalcin-like 1 is a key regulator of LDHA activation that promotes the growth of non-small cell lung carcinoma. In Cellular oncology (Dordrecht, Netherlands), 45, 179-191. doi:10.1007/s13402-022-00661-0. https://pubmed.ncbi.nlm.nih.gov/35102488/
7. Chen, Yun, Wang, Xiaoting, Zhao, Ping, Zhang, Yan, Cao, Brian. . Development and characterization of monoclonal antibodies against pancreatic cancer marker hippocalcin-like 1 protein. In Monoclonal antibodies in immunodiagnosis and immunotherapy, 33, 20-7. doi:10.1089/mab.2013.0074. https://pubmed.ncbi.nlm.nih.gov/24555932/
8. Hosseini, A, Sauerwein, H, Mielenz, M. 2010. Putative reference genes for gene expression studies in propionate and β-hydroxybutyrate treated bovine adipose tissue explants. In Journal of animal physiology and animal nutrition, 94, e178-84. doi:10.1111/j.1439-0396.2010.01002.x. https://pubmed.ncbi.nlm.nih.gov/20579188/
9. Li, Zhi, Na Wu, Xu, Jacquot, Aurore, Lejay, Laurence, Schulze, Waltraud X. . Phosphoregulation in the N-terminus of NRT2.1 affects nitrate uptake by controlling the interaction of NRT2.1 with NAR2.1 and kinase HPCAL1 in Arabidopsis. In Journal of experimental botany, 75, 2127-2142. doi:10.1093/jxb/erad490. https://pubmed.ncbi.nlm.nih.gov/38066636/