Wbp2-KO 基因敲除小鼠

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产品名称

Wbp2-KO 基因敲除小鼠

产品编号

S-KO-16688

品系全称

C57BL/6JCya-Wbp2em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-22378-Wbp2-B6J-VB

品系状态

使用本品系发表的文献需注明: Wbp2-KO 基因敲除小鼠 mice (Strain S-KO-16688) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
WW domain binding protein 2
基因别称
-
染色体号
Chr 11 (Mouse)
转录本 ID
NCBI: NM_016852 | Ensembl: ENSMUST00000074628
修饰方式
全身性基因敲除
靶向范围
Exon 2
敲除长度
~0.9 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:104709Mice homozygous for a null allele show progressive high-frequency hearing loss, raised auditory brainstem response (ABR) thresholds, reduced ABR amplitudes, swelling of afferent terminals, inner hair cell synapse defects, and altered expression of AMPA receptor subunits and post-synaptic proteins.
WBP2,也称为WW domain binding protein 2,是一种重要的转录共激活因子。它含有两个WW结构域,可以与含有PPxY基序的蛋白质相互作用。WBP2在多种生物学过程中发挥作用,包括细胞增殖、分化和凋亡。WBP2的表达和功能异常与多种疾病的发生和发展密切相关,包括乳腺癌、胃癌和非酒精性脂肪肝等。

WBP2在乳腺癌的发生和发展中发挥重要作用。WBP2可以与雌激素受体α(ERα)和孕激素受体(PR)相互作用,增强其转录活性,从而促进乳腺癌细胞的增殖和迁移[2]。此外,WBP2还可以与YAP/TAZ相互作用,激活下游信号通路,促进乳腺癌细胞的上皮-间质转化(EMT)和侵袭能力[2]。WBP2的表达水平与乳腺癌的恶性程度和临床耐药性密切相关[1]。WBP2还可以通过调节MDR1的转录,增强乳腺癌细胞对化疗药物的耐药性[3]。WBP2的表达水平与乳腺癌对曲妥珠单抗的敏感性相关,高表达WBP2的乳腺癌细胞对曲妥珠单抗的敏感性更高[4]。

WBP2在胃癌的发生和发展中也发挥重要作用。WBP2可以与LATS2相互作用,抑制其磷酸化,从而激活Hippo信号通路,促进胃癌细胞的迁移[5]。WBP2的表达水平与胃癌的临床预后相关,高表达WBP2的胃癌患者预后较差[5]。

WBP2在非酒精性脂肪肝的发生和发展中也发挥重要作用。WBP2可以与AMPKβ1相互作用,激活AMPK信号通路,从而抑制肝细胞的脂肪沉积和胰岛素抵抗[6]。

综上所述,WBP2是一种重要的转录共激活因子,参与调控多种生物学过程。WBP2的表达和功能异常与多种疾病的发生和发展密切相关,包括乳腺癌、胃癌和非酒精性脂肪肝等。深入研究WBP2的生物学功能和作用机制,有助于开发新的治疗策略,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Liu, Yan, He, Enping, Zhang, Yanling, Zeng, Youqing, Leng, Ping. . WW domain binding protein 2 (WBP2) as an oncogene in breast cancer: mechanisms and therapeutic prospects-a narrative review. In Gland surgery, 11, 1984-2002. doi:10.21037/gs-22-716. https://pubmed.ncbi.nlm.nih.gov/36654949/
2. Chen, Shuai, Wang, Han, Huang, Yu-Fan, Tzeng, Chi-Meng, Zhang, Zhi-Ming. 2017. WW domain-binding protein 2: an adaptor protein closely linked to the development of breast cancer. In Molecular cancer, 16, 128. doi:10.1186/s12943-017-0693-9. https://pubmed.ncbi.nlm.nih.gov/28724435/
3. Chen, Shuai, Wang, Han, Li, Zhi, Tzeng, Chi-Meng, Zhang, Zhi-Ming. 2018. Interaction of WBP2 with ERα increases doxorubicin resistance of breast cancer cells by modulating MDR1 transcription. In British journal of cancer, 119, 182-192. doi:10.1038/s41416-018-0119-5. https://pubmed.ncbi.nlm.nih.gov/29937544/
4. Kang, Shin-Ae, Guan, Jye Swei, Tan, Hock Jin, Lee, Soo Chin, Lim, Yoon Pin. 2018. Elevated WBP2 Expression in HER2-positive Breast Cancers Correlates with Sensitivity to Trastuzumab-based Neoadjuvant Therapy: A Retrospective and Multicentric Study. In Clinical cancer research : an official journal of the American Association for Cancer Research, 25, 2588-2600. doi:10.1158/1078-0432.CCR-18-3228. https://pubmed.ncbi.nlm.nih.gov/30593516/
5. Hum, Melissa, Tan, Hock Jin, Yang, Yixuan, Teh, Ming, Lim, Yoon Pin. . WBP2 promotes gastric cancer cell migration via novel targeting of LATS2 kinase in the Hippo tumor suppressor pathway. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35, e21290. doi:10.1096/fj.202000393R. https://pubmed.ncbi.nlm.nih.gov/33475198/
6. Zheng, Zhe, Li, Yue, Fan, Siyuan, Zhu, Feng, Huang, Kai. 2021. WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1. In Cell death & disease, 12, 228. doi:10.1038/s41419-021-03536-8. https://pubmed.ncbi.nlm.nih.gov/33658485/