Hipk1-KO 基因敲除小鼠

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产品名称

Hipk1-KO 基因敲除小鼠

产品编号

S-KO-16486

品系全称

C57BL/6JCya-Hipk1em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-15257-Hipk1-B6J-VB

品系状态

使用本品系发表的文献需注明: Hipk1-KO 基因敲除小鼠 mice (Strain S-KO-16486) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
homeodomain interacting protein kinase 1
基因别称
1110062K04Rik,Myak
染色体号
Chr 3 (Mouse)
转录本 ID
NCBI: NM_010432 | Ensembl: ENSMUST00000029438
修饰方式
全身性基因敲除
靶向范围
Exon 3
敲除长度
~0.9 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1314873Homozygous null mice are viable and fertile, do not develop spontaneous tumors, and are resistant to DMBA-induced skin tumor formation.
HIPK1,即Homeodomain Interacting Protein Kinase 1,是一种保守的丝氨酸/苏氨酸激酶,能够在细胞内响应不同的应激信号。HIPK1能够与同源结构域蛋白和其他转录因子相互作用,在转录调控中充当共激活剂或共阻遏物,参与调控多种生物过程,如信号转导、细胞凋亡、胚胎发育、DNA损伤反应和细胞增殖等。HIPK1在多种人类疾病中发挥重要作用,包括癌症、纤维化、糖尿病、肌萎缩侧索硬化症(ALS)、Rett综合征、小脑疾病和视网膜血管功能障碍等。

HIPK1在心脏疾病中的作用已被研究。研究发现,在病理性心脏肥大期间,HIPK1的表达会增加。通过基因敲除和基因治疗靶向HIPK1可以保护心脏免受病理性肥大和心力衰竭的影响。HIPK1的抑制可以通过抑制CREB-C/EBPβ轴来防止病理性心脏肥大[1]。此外,HIPK1还与长寿相关。研究发现,在UM-HET3小鼠中,HIPK1被鉴定为一种保守的长寿基因[2]。HIPK1在骨质疏松症和癌症中也发挥作用。研究发现,ROCK1作为骨质疏松症的生物标志物,与HIPK1相关[3]。另外,HIPK1在结肠癌中发挥重要作用,miR-770-5p通过下调HIPK1的表达来调节对甲氨蝶呤的耐药性[6]。

HIPK1还与细胞中的蛋白质表达相关。研究发现,通过过表达miR-22和敲除HIPK1基因可以改善HEK293细胞中的蛋白质表达[4][7]。此外,HIPK1与c-Myb相互作用并调节其活性[5]。HIPK1还与Wnt信号通路相关。研究发现,Wnt信号通路在发育和成年大脑中具有相反的作用,并且这些作用受到HIPK1的调节[8]。

综上所述,HIPK1是一种重要的丝氨酸/苏氨酸激酶,参与调控多种生物过程,并在多种人类疾病中发挥重要作用。HIPK1的研究有助于深入理解其在不同疾病中的作用机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Bei, Yihua, Zhu, Yujiao, Wei, Meng, Sluijter, Joost Pg, Xiao, Junjie. 2023. HIPK1 Inhibition Protects against Pathological Cardiac Hypertrophy by Inhibiting the CREB-C/EBPβ Axis. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2300585. doi:10.1002/advs.202300585. https://pubmed.ncbi.nlm.nih.gov/37098980/
2. Bou Sleiman, Maroun, Roy, Suheeta, Gao, Arwen W, Williams, Robert W, Auwerx, Johan. 2022. Sex- and age-dependent genetics of longevity in a heterogeneous mouse population. In Science (New York, N.Y.), 377, eabo3191. doi:10.1126/science.abo3191. https://pubmed.ncbi.nlm.nih.gov/36173858/
3. Lai, Bowen, Jiang, Heng, Gao, Yuan, Zhou, Xuhui. 2023. Identification of ROCK1 as a novel biomarker for postmenopausal osteoporosis and pan-cancer analysis. In Aging, 15, 8873-8907. doi:10.18632/aging.205004. https://pubmed.ncbi.nlm.nih.gov/37683138/
4. Inwood, Sarah, Abaandou, Laura, Betenbaugh, Michael, Shiloach, Joseph. 2019. Improved protein expression in HEK293 cells by over-expressing miR-22 and knocking-out its target gene, HIPK1. In New biotechnology, 54, 28-33. doi:10.1016/j.nbt.2019.08.004. https://pubmed.ncbi.nlm.nih.gov/31425885/
5. Matre, Vilborg, Nordgård, Oddmund, Alm-Kristiansen, Anne Hege, Ledsaak, Marit, Gabrielsen, Odd Stokke. 2009. HIPK1 interacts with c-Myb and modulates its activity through phosphorylation. In Biochemical and biophysical research communications, 388, 150-4. doi:10.1016/j.bbrc.2009.07.139. https://pubmed.ncbi.nlm.nih.gov/19646965/
6. Zhang, Dawei, Li, Ying, Sun, Peilong. 2019. miR-770-5p modulates resistance to methotrexate in human colorectal adenocarcinoma cells by downregulating HIPK1. In Experimental and therapeutic medicine, 19, 339-346. doi:10.3892/etm.2019.8221. https://pubmed.ncbi.nlm.nih.gov/31853309/
7. Inwood, Sarah, Buehler, Eugen, Betenbaugh, Michael, Lal, Madhu, Shiloach, Joseph. 2017. Identifying HIPK1 as Target of miR-22-3p Enhancing Recombinant Protein Production From HEK 293 Cell by Using Microarray and HTP siRNA Screen. In Biotechnology journal, 13, . doi:10.1002/biot.201700342. https://pubmed.ncbi.nlm.nih.gov/28987030/
8. Marinaro, Cinzia, Pannese, Maria, Weinandy, Franziska, Martino, Gianvito, Muzio, Luca. 2011. Wnt signaling has opposing roles in the developing and the adult brain that are modulated by Hipk1. In Cerebral cortex (New York, N.Y. : 1991), 22, 2415-27. doi:10.1093/cercor/bhr320. https://pubmed.ncbi.nlm.nih.gov/22095214/