Clec4e-KO 基因敲除小鼠

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产品名称

Clec4e-KO 基因敲除小鼠

产品编号

S-KO-15940

品系全称

C57BL/6JCya-Clec4eem1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-56619-Clec4e-B6J-VA

品系状态

使用本品系发表的文献需注明: Clec4e-KO 基因敲除小鼠 mice (Strain S-KO-15940) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
C-type lectin domain family 4, member e
基因别称
Clecsf9,Mincle
染色体号
Chr 6 (Mouse)
转录本 ID
NCBI: NM_019948 | Ensembl: ENSMUST00000032239
修饰方式
全身性基因敲除
靶向范围
Exon 3~4
敲除长度
~1.4 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1861232Mice homozygous for a null mutation exhibit reduced cytokine (TNF) production after challenge with C. albicans and are more susceptible to systemic candidiasis. The majority of homozygotes also display histological evidence of abnormal heart valves.
CLEC4E,也称为Mincle(巨噬细胞诱导的C型凝集素,Macrophage-inducible C-type lectin),是一种重要的C型凝集素受体。C型凝集素受体是一类模式识别受体,能够识别病原体相关分子模式(PAMPs),从而启动免疫反应。Mincle主要在巨噬细胞和树突状细胞中表达,能够识别多种病原体成分,包括真菌、细菌和某些病毒[1,2]。

Mincle在抗感染免疫中发挥着重要作用。例如,Mincle能够识别分枝杆菌的细胞壁成分TDM(trehalose-6,6-dimycolate),并激活Syk-Card9信号通路,从而促进巨噬细胞对分枝杆菌的吞噬和杀灭[3,4]。此外,Mincle还能够识别某些真菌的细胞壁成分,如甘露聚糖,并启动相应的免疫反应[5]。

除了在抗感染免疫中的作用,Mincle还参与多种疾病的发病机制。例如,Mincle的表达与动脉粥样硬化的发生发展相关。Mincle能够识别氧化低密度脂蛋白(oxLDL)并促进巨噬细胞的炎症反应,从而促进动脉粥样硬化斑块的形成[6]。此外,Mincle的表达还与肺结核的易感性相关。一些研究表明,CLEC4E基因的多态性与肺结核的易感性相关,其中一些多态性可能增加个体对肺结核的易感性,而另一些多态性可能提供保护作用[7,8,9]。

除了在免疫和疾病中的作用,Mincle还参与其他生物学过程。例如,Mincle的表达与细胞自噬相关。一些研究表明,Mincle能够诱导巨噬细胞自噬,从而限制病原体的生长和复制[10]。此外,Mincle的表达还与脂肪肝的发生发展相关。一些研究表明,Mincle的表达与脂肪肝的发生发展相关,可能是脂肪肝的潜在生物标志物[11]。

综上所述,CLEC4E(Mincle)是一种重要的C型凝集素受体,参与抗感染免疫、动脉粥样硬化、肺结核、自噬和脂肪肝等多种生物学过程。Mincle的研究有助于深入理解免疫系统的功能和疾病发生机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Kabuye, Deo, Chu, Yang, Lao, Wenting, Jin, Guojiang, Kang, Hui. 2019. Association between CLEC4E gene polymorphism of mincle and pulmonary tuberculosis infection in a northern Chinese population. In Gene, 710, 24-29. doi:10.1016/j.gene.2019.05.011. https://pubmed.ncbi.nlm.nih.gov/31075410/
2. Bowker, Nicholas, Salie, Muneeb, Schurz, Haiko, Hoal, Eileen G, Möller, Marlo. 2016. Polymorphisms in the Pattern Recognition Receptor Mincle Gene (CLEC4E) and Association with Tuberculosis. In Lung, 194, 763-7. doi:10.1007/s00408-016-9915-y. https://pubmed.ncbi.nlm.nih.gov/27363694/
3. Olvany, Jasmine M, Sausville, Lindsay N, White, Marquitta J, Williams, Scott M, Sirugo, Giorgio. 2020. CLEC4E (Mincle) genetic variation associates with pulmonary tuberculosis in Guinea-Bissau (West Africa). In Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 85, 104560. doi:10.1016/j.meegid.2020.104560. https://pubmed.ncbi.nlm.nih.gov/32971250/
4. Pahari, Susanta, Negi, Shikha, Aqdas, Mohammad, Schlesinger, Larry S, Agrewala, Javed N. 2019. Induction of autophagy through CLEC4E in combination with TLR4: an innovative strategy to restrict the survival of Mycobacterium tuberculosis. In Autophagy, 16, 1021-1043. doi:10.1080/15548627.2019.1658436. https://pubmed.ncbi.nlm.nih.gov/31462144/
5. Cheng, Shuai, Liu, Yuanlin, Jing, Yuchen, Jia, Longyuan, Xin, Shijie. 2022. Identification of key monocytes/macrophages related gene set of the early-stage abdominal aortic aneurysm by integrated bioinformatics analysis and experimental validation. In Frontiers in cardiovascular medicine, 9, 950961. doi:10.3389/fcvm.2022.950961. https://pubmed.ncbi.nlm.nih.gov/36186997/
6. Zhou, Wenjing, Wu, Lijuan, Song, Jiajia, Wu, Tao, Ying, Binwu. 2021. A case-control study on correlation between the single nucleotide polymorphism of CLEC4E and the susceptibility to tuberculosis among Han people in Western China. In BMC infectious diseases, 21, 788. doi:10.1186/s12879-021-06448-2. https://pubmed.ncbi.nlm.nih.gov/34376176/
7. Sheikh Beig Goharrizi, Mohammad Ali, Ghodsi, Saeed, Mokhtari, Majid, Moravveji, Sayyed Sajjad. 2023. Non-invasive STEMI-related biomarkers based on meta-analysis and gene prioritization. In Computers in biology and medicine, 161, 106997. doi:10.1016/j.compbiomed.2023.106997. https://pubmed.ncbi.nlm.nih.gov/37216774/
8. He, Haidong, Zhu, Yifan, Ji, Xiaoguo, Pu, Suying, Zheng, Hui. 2025. The miR-22-5p/Clec4e axis has diagnostic potential in fructose-induced nonalcoholic fatty liver disease. In Biochemical and biophysical research communications, 753, 151496. doi:10.1016/j.bbrc.2025.151496. https://pubmed.ncbi.nlm.nih.gov/39978254/
9. Kang, Zhi-Ying, Huang, Qian-Yu, Zhen, Ning-Xin, Zhang, Zhao-Cai, Tian, Bao-Ping. 2024. Heterogeneity of immune cells and their communications unveiled by transcriptome profiling in acute inflammatory lung injury. In Frontiers in immunology, 15, 1382449. doi:10.3389/fimmu.2024.1382449. https://pubmed.ncbi.nlm.nih.gov/38745657/
10. Gobbi, Giuliana, Carubbi, Cecilia, Tagliazucchi, Guidantonio Malagoli, Vitale, Marco, Ardissino, Diego. 2019. Sighting acute myocardial infarction through platelet gene expression. In Scientific reports, 9, 19574. doi:10.1038/s41598-019-56047-0. https://pubmed.ncbi.nlm.nih.gov/31863085/