Sucnr1-KO 基因敲除小鼠

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产品名称

Sucnr1-KO 基因敲除小鼠

产品编号

S-KO-15426

品系全称

C57BL/6NCya-Sucnr1em1/Cya

品系背景

C57BL/6NCya

品系编号

KOCMP-84112-Sucnr1-B6N-VA

品系状态

使用本品系发表的文献需注明: Sucnr1-KO 基因敲除小鼠 mice (Strain S-KO-15426) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
succinate receptor 1
基因别称
Gpr91
染色体号
Chr 3 (Mouse)
转录本 ID
NCBI: NM_032400 | Ensembl: ENSMUST00000029326
修饰方式
全身性基因敲除
靶向范围
Exon 2
敲除长度
~1.5 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1934135Mice homozygous for a knockout allele exhibit decreased renin plasma and kidney levels upon high-glucose stimulation in a diabetic or non-diabetic model.

发表文献

Applied and Environmental Microbiology
2022-03-21
A Murine Commensal Protozoan Influences Host Glucose Homeostasis by Facilitating Free Choline Generation
1
SUCNR1,也称为GPR91,是一种G蛋白偶联受体,主要在巨噬细胞中表达。它能够感知细胞外琥珀酸,并将其作为一种代谢应激信号来调节细胞功能。SUCNR1的激活通过Gq蛋白信号通路,影响转录调控,从而调节免疫细胞的极化和功能。此外,SUCNR1还参与调节肾脏纤维化和肠损伤等病理过程。

在巨噬细胞中,SUCNR1的激活通过Gq信号通路,促进转录免疫功能基因,从而调节巨噬细胞的M2极化[1]。SUCNR1的激活还能够控制巨噬细胞的抗炎反应,调节代谢反应,从而减轻肥胖引起的炎症和代谢紊乱[2]。此外,SUCNR1还参与调节肠道菌群和肺损伤,通过影响巨噬细胞极化,加剧肠缺血再灌注引起的急性肺损伤[3]。

在肾脏中,SUCNR1的激活通过M2巨噬细胞的极化,促进肾间质纤维化。琥珀酸的积累能够引起肾脏损伤,导致肾小管细胞凋亡和肾功能下降[4,5]。此外,SUCNR1的表达与卵巢癌患者的肿瘤浸润淋巴细胞和免疫相关基因表达相关,SUCNR1的高表达预示着卵巢癌患者的预后不良[7]。

SUCNR1的激活和功能还受到遗传因素的影响。例如,SUCNR1基因的单核苷酸多态性(SNP)rs73168929与2型糖尿病和高血压的易感性相关[6]。此外,SNP rs13079080能够影响miRNA-4470对SUCNR1基因的调节,进而影响SUCNR1的表达和功能[8]。

综上所述,SUCNR1是一种重要的G蛋白偶联受体,参与调节免疫细胞的极化和功能,以及肾脏和肠道损伤等病理过程。SUCNR1的激活和功能受到遗传因素的影响,其研究有助于深入理解代谢应激信号和免疫调节的机制,为相关疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Trauelsen, Mette, Hiron, Thomas K, Lin, Da, O'Callaghan, Chris A, Schwartz, Thue W. . Extracellular succinate hyperpolarizes M2 macrophages through SUCNR1/GPR91-mediated Gq signaling. In Cell reports, 35, 109246. doi:10.1016/j.celrep.2021.109246. https://pubmed.ncbi.nlm.nih.gov/34133934/
2. Keiran, Noelia, Ceperuelo-Mallafré, Victoria, Calvo, Enrique, Vendrell, Joan, Fernández-Veledo, Sonia. 2019. SUCNR1 controls an anti-inflammatory program in macrophages to regulate the metabolic response to obesity. In Nature immunology, 20, 581-592. doi:10.1038/s41590-019-0372-7. https://pubmed.ncbi.nlm.nih.gov/30962591/
3. Wang, Yi-Heng, Yan, Zheng-Zheng, Luo, Si-Dan, Li, Cai, Liu, Ke-Xuan. 2023. Gut microbiota-derived succinate aggravates acute lung injury after intestinal ischaemia/reperfusion in mice. In The European respiratory journal, 61, . doi:10.1183/13993003.00840-2022. https://pubmed.ncbi.nlm.nih.gov/36229053/
4. Pu, Min, Zhang, Jing, Hong, Fuyan, Gao, Guoquan, Zhou, Ti. 2024. The pathogenic role of succinate-SUCNR1: a critical function that induces renal fibrosis via M2 macrophage. In Cell communication and signaling : CCS, 22, 78. doi:10.1186/s12964-024-01481-5. https://pubmed.ncbi.nlm.nih.gov/38291510/
5. Pu, Min, Zhang, Jing, Zeng, Yongcheng, Gao, Guoquan, Zhou, Ti. 2023. Succinate-SUCNR1 induces renal tubular cell apoptosis. In American journal of physiology. Cell physiology, 324, C467-C476. doi:10.1152/ajpcell.00327.2022. https://pubmed.ncbi.nlm.nih.gov/36622070/
6. Du, Bingxin, Jia, Xinhui, Tian, Wenqi, Qiu, Changchun, Zhang, Qi. 2020. Associations of SUCNR1, GRK4, CAMK1D gene polymorphisms and the susceptibility of type 2 diabetes mellitus and essential hypertension in a northern Chinese Han population. In Journal of diabetes and its complications, 35, 107752. doi:10.1016/j.jdiacomp.2020.107752. https://pubmed.ncbi.nlm.nih.gov/33127268/
7. Zhang, Jiawen, Zhang, Qinyi, Yang, Yongbin, Wang, Qingying. 2020. Association Between Succinate Receptor SUCNR1 Expression and Immune Infiltrates in Ovarian Cancer. In Frontiers in molecular biosciences, 7, 150. doi:10.3389/fmolb.2020.00150. https://pubmed.ncbi.nlm.nih.gov/33062639/
8. Louer, Elja M M, Lorés-Motta, Laura, Ion, Ana Mãdãlina, Den Hollander, Anneke I, Deen, Peter M T. 2019. Single nucleotide polymorphism rs13079080 is associated with differential regulation of the succinate receptor 1 (SUCNR1) gene by miRNA-4470. In RNA biology, 16, 1547-1554. doi:10.1080/15476286.2019.1643100. https://pubmed.ncbi.nlm.nih.gov/31304868/

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