Uba7-KO 基因敲除小鼠

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产品名称

Uba7-KO 基因敲除小鼠

产品编号

S-KO-14312

品系全称

C57BL/6JCya-Uba7em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-74153-Uba7-B6J-VA

品系状态

使用本品系发表的文献需注明: Uba7-KO 基因敲除小鼠 mice (Strain S-KO-14312) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
ubiquitin-like modifier activating enzyme 7
基因别称
1300004C08Rik,Ube1l
染色体号
Chr 9 (Mouse)
转录本 ID
NCBI: NM_023738.4 | Ensembl: ENSMUST00000035216
修饰方式
全身性基因敲除
靶向范围
Exon 2~18
敲除长度
~4.4 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1349462Homozygous null mice lacking ISG15 conjugation but not free ISG15 are healthy and fertile and exhibit normal antiviral responses against vesicular stomatitis virus and lymphocytic choriomeningitis virus infection. Bone-derived macrophages from mutant mice display normal responses to IFN treatment.
Uba7,即Ubiquitin-like modifier-activating enzyme 7,是一种重要的E1-like ubiquitin-activating酶,参与ISG15(Interferon-stimulated gene 15)的共价结合。ISG15是一种在干扰素(IFN)刺激下表达的蛋白质,它通过ISGylation这一过程与细胞蛋白共价结合,从而影响多种生物学过程,包括细胞信号传导、蛋白质稳定性、免疫反应和抗病毒防御等。Uba7在ISGylation过程中起着关键作用,它激活ISG15并将其转移到细胞蛋白上,这一过程需要E2-conjugating酶和E3 ligase的参与。

Uba7在多种生物学过程中发挥着重要作用。在抗病毒防御方面,Uba7和ISG15共同抑制病毒复制。研究发现,Uba7和ISG15的表达下调会增加狂犬病毒(RABV)的滴度,表明它们具有抗病毒功能[2]。此外,Uba7还参与肿瘤抑制。在乳腺癌中,Uba7的表达下调与肿瘤生长和转移相关,表明Uba7是一种潜在的肿瘤抑制基因[1,3]。Uba7还参与免疫反应的调节。在LPS刺激的微胶质细胞中,ISGylation可以增加多种蛋白质的稳定性,包括Stat1,从而防止免疫反应的过早终止[4]。此外,Uba7还与精神疾病相关。研究发现,Uba7的表达与创伤后应激障碍(PTSD)和重度抑郁症(MDD)相关,表明Uba7可能参与精神疾病的发病机制[5,6]。

Uba7的生物学功能是多方面的,它不仅参与ISGylation过程,还与肿瘤抑制、免疫反应和神经精神疾病相关。这些发现为Uba7在疾病发生机制中的作用提供了新的线索,也为疾病的治疗和预防提供了新的思路和策略。

参考文献:
1. Fan, Jun-Bao, Miyauchi, Sayuri, Xu, Hui-Zhong, Fu, Xiang-Dong, Zhang, Dong-Er. 2020. Type I Interferon Regulates a Coordinated Gene Network to Enhance Cytotoxic T Cell-Mediated Tumor Killing. In Cancer discovery, 10, 382-393. doi:10.1158/2159-8290.CD-19-0608. https://pubmed.ncbi.nlm.nih.gov/31974171/
2. Zhao, Pingsen, Jiang, Tianqi, Zhong, Zhixiong, Yang, Songtao, Xia, Xianzhu. 2017. Inhibition of rabies virus replication by interferon-stimulated gene 15 and its activating enzyme UBA7. In Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 56, 44-53. doi:10.1016/j.meegid.2017.10.016. https://pubmed.ncbi.nlm.nih.gov/29056542/
3. Lin, Meng, Li, Yanqing, Qin, Shanshan, Jiao, Yan, Hua, Fang. 2020. Ubiquitin-like modifier-activating enzyme 7 as a marker for the diagnosis and prognosis of breast cancer. In Oncology letters, 19, 2773-2784. doi:10.3892/ol.2020.11406. https://pubmed.ncbi.nlm.nih.gov/32218830/
4. Przanowski, Piotr, Loska, Stefan, Cysewski, Dominik, Dabrowski, Michal, Kaminska, Bozena. 2017. ISG'ylation increases stability of numerous proteins including Stat1, which prevents premature termination of immune response in LPS-stimulated microglia. In Neurochemistry international, 112, 227-233. doi:10.1016/j.neuint.2017.07.013. https://pubmed.ncbi.nlm.nih.gov/28774718/
5. Çalışkan, Minal, Baker, Samuel W, Gilad, Yoav, Ober, Carole. 2015. Host genetic variation influences gene expression response to rhinovirus infection. In PLoS genetics, 11, e1005111. doi:10.1371/journal.pgen.1005111. https://pubmed.ncbi.nlm.nih.gov/25874939/
6. Garrett, Melanie E, Qin, Xue Jun, Mehta, Divya, Hauser, Michael A, Ashley-Koch, Allison E. 2021. Gene Expression Analysis in Three Posttraumatic Stress Disorder Cohorts Implicates Inflammation and Innate Immunity Pathways and Uncovers Shared Genetic Risk With Major Depressive Disorder. In Frontiers in neuroscience, 15, 678548. doi:10.3389/fnins.2021.678548. https://pubmed.ncbi.nlm.nih.gov/34393704/