Hnrnpll-KO 基因敲除小鼠

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产品名称

Hnrnpll-KO 基因敲除小鼠

产品编号

S-KO-14011

品系全称

C57BL/6JCya-Hnrnpllem1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-72692-Hnrnpll-B6J-VA

品系状态

使用本品系发表的文献需注明: Hnrnpll-KO 基因敲除小鼠 mice (Strain S-KO-14011) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
heterogeneous nuclear ribonucleoprotein L-like
基因别称
2510028H02Rik,2810036L13Rik,Hnrpll
染色体号
Chr 17 (Mouse)
转录本 ID
NCBI: NM_144802 | Ensembl: ENSMUST00000184635
修饰方式
全身性基因敲除
靶向范围
Exon 3
敲除长度
~1.0 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1919942Mice homozygous for a point mutation in a RNA recognition motif of the gene product have defects in the generation of alternative transcripts normally found in memory T cells. Total CD4+ T cell counts are lower, with a reduction of nave CD44lo T cells occurring as mice age.
Hnrnpll,也称为hnRNPLL,是一种异核RNA结合蛋白L类似物。它主要存在于细胞核中,并且作为一种RNA结合蛋白,它在调控RNA代谢过程中发挥着重要作用。Hnrnpll的主要功能是促进前mRNA的剪接,但它在RNA代谢的所有过程中都发挥着作用,通过识别RNA上的特定顺式作用元件[1]。此外,Hnrnpll还参与调控细胞分化和维持淋巴细胞的稳态。

在淋巴细胞的分化和发育过程中,Hnrnpll发挥着重要作用。例如,在记忆T细胞的发展过程中,Hnrnpll可以促进CD45RA向CD45RO的转换。这个过程对于T细胞的分化和功能至关重要[2]。此外,Hnrnpll还控制着淋巴细胞发育过程中关键基因的替代剪接或表达,从而调节淋巴细胞的稳态和终末分化[3]。

除了在淋巴细胞中的作用外,Hnrnpll还在胚胎干细胞的多能性退出过程中发挥重要作用。研究表明,Hnrnpll可以通过调节Tbx3和Bptf两个转录因子的替代剪接,从而控制胚胎干细胞的多能性退出[4]。此外,Hnrnpll还参与调节B细胞的分化和功能。例如,Hnrnpll可以调控B细胞受体和Toll样受体介导的刺激下B细胞的增殖,以及B细胞向浆细胞分化的过程[5]。

Hnrnpll还在肿瘤发生和转移过程中发挥重要作用。研究表明,Hnrnpll可以作为结直肠癌转移的抑制因子,通过调节CD44的替代剪接来抑制肿瘤的转移[6]。此外,Hnrnpll还可以通过调节DNA复制相关蛋白的mRNA稳定性和细胞周期进程来促进结直肠癌细胞增殖[7]。

除了在免疫系统和肿瘤发生中的作用外,Hnrnpll还在神经系统中发挥作用。研究表明,Hnrnpll的突变可以导致记忆T细胞的积累减少,并且还会影响小鼠的行为表现,如增强的抑郁样行为和空间记忆障碍[8]。此外,Hnrnpll还可以通过诱导特定内含子的保留来调控T细胞中的替代剪接程序[9]。这些发现表明,Hnrnpll在免疫、肿瘤和神经系统等多个生物学过程中发挥着重要作用。

综上所述,Hnrnpll是一种重要的RNA结合蛋白,它在调控RNA代谢、细胞分化和维持淋巴细胞的稳态方面发挥着重要作用。Hnrnpll还在肿瘤发生、转移和神经系统中发挥作用。Hnrnpll的研究有助于深入理解RNA代谢和细胞分化的调控机制,为相关疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Chang, Xing. 2016. RNA-binding protein hnRNPLL as a critical regulator of lymphocyte homeostasis and differentiation. In Wiley interdisciplinary reviews. RNA, 7, 295-302. doi:10.1002/wrna.1335. https://pubmed.ncbi.nlm.nih.gov/26821996/
2. Wang, Xue, Ping, Changyun, Tan, Puwen, Ma, Yanni, Huang, Yue. 2020. hnRNPLL controls pluripotency exit of embryonic stem cells by modulating alternative splicing of Tbx3 and Bptf. In The EMBO journal, 40, e104729. doi:10.15252/embj.2020104729. https://pubmed.ncbi.nlm.nih.gov/33349972/
3. Yabas, Mehmet, Yazicioglu, Yavuz F, Hoyne, Gerard F, Goodnow, Christopher C, Enders, Anselm. 2021. Loss of hnRNPLL-dependent splicing of Ptprc has no impact on B-cell development, activation and terminal differentiation into antibody-secreting cells. In Immunology and cell biology, 99, 532-541. doi:10.1111/imcb.12433. https://pubmed.ncbi.nlm.nih.gov/33331104/
4. Sakuma, Keiichiro, Sasaki, Eiichi, Kimura, Kenya, Yatabe, Yasushi, Aoki, Masahiro. 2017. HNRNPLL, a newly identified colorectal cancer metastasis suppressor, modulates alternative splicing of CD44 during epithelial-mesenchymal transition. In Gut, 67, 1103-1111. doi:10.1136/gutjnl-2016-312927. https://pubmed.ncbi.nlm.nih.gov/28360095/
5. Chang, Xing, Li, Bin, Rao, Anjana. 2015. RNA-binding protein hnRNPLL regulates mRNA splicing and stability during B-cell to plasma-cell differentiation. In Proceedings of the National Academy of Sciences of the United States of America, 112, E1888-97. doi:10.1073/pnas.1422490112. https://pubmed.ncbi.nlm.nih.gov/25825742/
6. Yin, Huijing, Wang, Jingshu, Li, Hui, Ren, Chunxia, Yang, Gong. 2021. Extracellular matrix protein-1 secretory isoform promotes ovarian cancer through increasing alternative mRNA splicing and stemness. In Nature communications, 12, 4230. doi:10.1038/s41467-021-24315-1. https://pubmed.ncbi.nlm.nih.gov/34244494/
7. Sakuma, Keiichiro, Sasaki, Eiichi, Kimura, Kenya, Yatabe, Yasushi, Aoki, Masahiro. 2018. HNRNPLL stabilizes mRNA for DNA replication proteins and promotes cell cycle progression in colorectal cancer cells. In Cancer science, 109, 2458-2468. doi:10.1111/cas.13660. https://pubmed.ncbi.nlm.nih.gov/29869816/
8. van den Buuse, Maarten, Halley, Paul, Hoyne, Gerard F. 2020. Behavioural phenotyping of thunder mice with a hypomorphic mutation of heterogeneous nuclear ribonuclear protein L-like (hnRNPLL) and reduced T cell function. In Neuroscience letters, 740, 135469. doi:10.1016/j.neulet.2020.135469. https://pubmed.ncbi.nlm.nih.gov/33152455/
9. Cho, Vicky, Mei, Yan, Sanny, Arleen, Goodnow, Christopher C, Andrews, T Daniel. 2014. The RNA-binding protein hnRNPLL induces a T cell alternative splicing program delineated by differential intron retention in polyadenylated RNA. In Genome biology, 15, R26. doi:10.1186/gb-2014-15-1-r26. https://pubmed.ncbi.nlm.nih.gov/24476532/