Gcnt3-KO 基因敲除小鼠

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产品名称

Gcnt3-KO 基因敲除小鼠

产品编号

S-KO-13819

品系全称

C57BL/6JCya-Gcnt3em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-72077-Gcnt3-B6J-VA

品系状态

使用本品系发表的文献需注明: Gcnt3-KO 基因敲除小鼠 mice (Strain S-KO-13819) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
glucosaminyl (N-acetyl) transferase 3, mucin type
基因别称
2010013H22Rik,2210021I22Rik,2210401J11Rik
染色体号
Chr 9 (Mouse)
转录本 ID
NCBI: NM_028087 | Ensembl: ENSMUST00000034751
修饰方式
全身性基因敲除
靶向范围
Exon 3
敲除长度
~1.3 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1919327Homozygote null mice have decreases in core 2 O-glycan structures on cell surfaces, decreased immunoglobulin levels, and disrupted mucosal barrier in the intestines.
GCNT3,也称为N-乙酰氨基葡萄糖转移酶3,是一种重要的糖基转移酶。糖基化是一种普遍存在于蛋白质和糖蛋白上的翻译后修饰,参与调控蛋白质的稳定性和功能,影响细胞信号传导、细胞粘附、细胞增殖和细胞迁移等生物学过程。GCNT3与另一个蛋白质GCNT1形成复合物,共同催化N-乙酰氨基葡萄糖基的转移。N-乙酰氨基葡萄糖基修饰在许多生物学过程中发挥作用,包括细胞分化、发育、代谢和疾病发生。

GCNT3在多种癌症中发挥重要作用,包括肺癌、结直肠癌、胰腺癌、肝细胞癌、前列腺癌和黑色素瘤。在肺癌中,GCNT3通过促进细胞增殖、迁移和侵袭来促进肺癌的进展[1]。在结直肠癌中,GCNT3的表达水平与患者的预后相关,低表达GCNT3的患者复发风险更高[2]。在胰腺癌中,GCNT3的表达水平与患者的预后相关,GCNT3的高表达与胰腺癌的进展和转移相关[3]。在肝细胞癌中,GCNT3通过调节MUC13的表达和GSK3β/β-catenin信号通路来促进肝细胞癌的进展[4]。在前列腺癌中,GCNT3的表达水平与患者的预后相关,GCNT3的高表达与前列腺癌的侵袭和转移相关[5]。在黑色素瘤中,GCNT3通过调节MCAM的表达和S100A8/A9信号通路来促进黑色素瘤的迁移和侵袭[6]。

GCNT3的表达和功能受到多种因素的调控,包括长链非编码RNA LINC00511、miR-195-5p和miR-302b-3p。LINC00511通过上调GCNT3的表达来促进肺癌的进展[7]。miR-195-5p通过下调GCNT3的表达来抑制结直肠癌的复发[2]。miR-302b-3p通过下调GCNT3的表达来抑制非小细胞肺癌的增殖、迁移和侵袭[8]。

综上所述,GCNT3是一种重要的糖基转移酶,参与调控蛋白质的糖基化和细胞生物学过程。GCNT3在多种癌症中发挥重要作用,包括肺癌、结直肠癌、胰腺癌、肝细胞癌、前列腺癌和黑色素瘤。GCNT3的表达和功能受到多种因素的调控,包括长链非编码RNA和miRNA。GCNT3的研究有助于深入理解糖基化的生物学功能和癌症发生机制,为癌症的治疗和预防提供新的思路和策略。

参考文献:
1. Zhao, Teng, Zhao, Xin, Qian, Kun, Gu, Yanfei, Zhang, Yi. 2022. Radiotherapy prognosis-associated gene GCNT3 promotes the proliferation, migration and invasion of lung adenocarcinoma cells. In Heliyon, 8, e12100. doi:10.1016/j.heliyon.2022.e12100. https://pubmed.ncbi.nlm.nih.gov/36578381/
2. González-Vallinas, Margarita, Vargas, Teodoro, Moreno-Rubio, Juan, Feliu, Jaime, Ramírez de Molina, Ana. 2014. Clinical relevance of the differential expression of the glycosyltransferase gene GCNT3 in colon cancer. In European journal of cancer (Oxford, England : 1990), 51, 1-8. doi:10.1016/j.ejca.2014.10.021. https://pubmed.ncbi.nlm.nih.gov/25466507/
3. Gupta, Rohitesh, Leon, Frank, Thompson, Christopher M, Kumar, Sushil, Ponnusamy, Moorthy P. 2020. Global analysis of human glycosyltransferases reveals novel targets for pancreatic cancer pathogenesis. In British journal of cancer, 122, 1661-1672. doi:10.1038/s41416-020-0772-3. https://pubmed.ncbi.nlm.nih.gov/32203219/
4. Kang, Qiu, Tingting, Wu, Bingzi, Dong, Chuandong, Sun, Chengzhan, Zhu. 2024. GCNT3 regulated MUC13 to promote the development of hepatocellular carcinoma through the GSK3β/β-catenin pathway. In Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, 56, 1572-1581. doi:10.1016/j.dld.2024.01.198. https://pubmed.ncbi.nlm.nih.gov/38369410/
5. Zhang, Youyi, Xiao, Ping, Hu, Xiaobo. 2022. LINC00511 enhances LUAD malignancy by upregulating GCNT3 via miR-195-5p. In BMC cancer, 22, 389. doi:10.1186/s12885-022-09459-7. https://pubmed.ncbi.nlm.nih.gov/35399076/
6. Yamamoto, Daiki, Sasaki, Katsumasa, Kosaka, Takeo, Oya, Mototsugu, Sato, Toshinori. . Functional analysis of GCNT3 for cell migration and EMT of castration-resistant prostate cancer cells. In Glycobiology, 32, 897-908. doi:10.1093/glycob/cwac044. https://pubmed.ncbi.nlm.nih.gov/35867813/
7. Li, Qian, Ran, Pengzhan, Zhang, Xiyu, Zheng, Shangyong, Xiao, Chunjie. 2018. Downregulation of N-Acetylglucosaminyltransferase GCNT3 by miR-302b-3p Decreases Non-Small Cell Lung Cancer (NSCLC) Cell Proliferation, Migration and Invasion. In Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 50, 987-1004. doi:10.1159/000494482. https://pubmed.ncbi.nlm.nih.gov/30355927/
8. Wang, Min, She, Wenyu, Yang, Jun, Zhang, Xu, Zhang, Luo. 2020. Gene Expression Analysis by Real-Time PCR in Nasal Brushings of Adult Patients with Allergic Rhinitis, Suspected Allergic Rhinitis, and Nonallergic Rhinitis. In International archives of allergy and immunology, 182, 301-310. doi:10.1159/000510942. https://pubmed.ncbi.nlm.nih.gov/33171477/