Hgh1-KO 基因敲除小鼠

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产品名称

Hgh1-KO 基因敲除小鼠

产品编号

S-KO-11178

品系全称

C57BL/6JCya-Hgh1em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-59053-Hgh1-B6J-VA

品系状态

使用本品系发表的文献需注明: Hgh1-KO 基因敲除小鼠 mice (Strain S-KO-11178) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
HGH1 homolog
基因别称
Brp16,D15Ertd741e,Fam203a,MNCb-5873
染色体号
Chr 15 (Mouse)
转录本 ID
NCBI: NM_021555.2 | Ensembl: ENSMUST00000023213
修饰方式
全身性基因敲除
靶向范围
Exon 1~6
敲除长度
~1726 bp
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
Hgh1,也称为人类生长激素基因1,是编码生长激素(GH)的基因。生长激素是一种重要的肽激素,参与调节人体的生长、发育和代谢。Hgh1的表达受到多种因素的调控,包括营养状态、生理节律和激素水平等。生长激素的异常表达与多种疾病的发生和发展相关,包括矮小症、巨人症、糖尿病和肿瘤等。

根据文献报道,NSUN2和YBX1通过m5C修饰增强HGH1 mRNA的稳定性,从而促进乳腺癌的进展[1]。HGH1的表达与乳腺癌的预后和免疫反应相关,高水平的HGH1表达与不良的临床特征和预后相关[2]。HGH1在乳腺癌细胞中高表达,并参与调节细胞周期和免疫反应。HGH1的表达水平与Th2细胞浸润正相关,与Tcm细胞浸润负相关[3]。HGH1的敲低可以抑制乳腺癌细胞的增殖、侵袭和迁移[4]。

HGH1在结直肠癌中发挥重要作用,LncRNA CASC21通过诱导HGH1的表达促进结直肠癌细胞的增殖、迁移、EMT和干性[5]。胰岛素通过HIF-1依赖性下调HGH1的表达[6]。Hsp90和cochaperones在调节eEF2功能中发挥重要作用,Hgh1作为一种cochaperone,参与eEF2的折叠和功能[7]。通过基因递送系统,可以将hGH1基因递送到脑毛细血管内皮细胞中,从而促进蛋白质的分泌[8]。HGH1基因的缺失与孤立性生长激素缺乏症相关[9]。

综上所述,Hgh1是一种重要的基因,其表达受到多种因素的调控,参与调节人体的生长、发育和代谢。Hgh1的异常表达与多种疾病的发生和发展相关,包括矮小症、巨人症、糖尿病和肿瘤等。Hgh1在肿瘤中的作用机制复杂,涉及到mRNA的稳定性、蛋白质的翻译和免疫反应等多个层面。Hgh1的研究有助于深入理解生长激素的生物学功能和疾病发生机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Zhang, Xuran, An, Ke, Ge, Xin, Kan, Quancheng, Tian, Xin. 2024. NSUN2/YBX1 promotes the progression of breast cancer by enhancing HGH1 mRNA stability through m5C methylation. In Breast cancer research : BCR, 26, 94. doi:10.1186/s13058-024-01847-0. https://pubmed.ncbi.nlm.nih.gov/38844963/
2. Wu, Gujie, Dong, Yipeng, Hu, Qin, Yang, Zheng, He, Min. 2023. HGH1 and the immune landscape: a novel prognostic marker for immune-desert tumor microenvironment identification and immunotherapy outcome prediction in human cancers. In Cell cycle (Georgetown, Tex.), 22, 1969-1985. doi:10.1080/15384101.2023.2260163. https://pubmed.ncbi.nlm.nih.gov/37811868/
3. Li, Hailong, Xu, Yong, Xu, Rong, Du, Wei. 2024. The role of HGH1 in breast cancer prognosis: a study on immune response and cell cycle. In BMC cancer, 24, 1122. doi:10.1186/s12885-024-12879-2. https://pubmed.ncbi.nlm.nih.gov/39251967/
4. Vakili, Hana, Jin, Yan, Cattini, Peter A. 2016. Evidence for a Circadian Effect on the Reduction of Human Growth Hormone Gene Expression in Response to Excess Caloric Intake. In The Journal of biological chemistry, 291, 13823-33. doi:10.1074/jbc.M116.722744. https://pubmed.ncbi.nlm.nih.gov/27151213/
5. Wang, Zeyu, Liu, Taiyuan, He, Kang, Zhang, Yingchao, Zhao, Lijing. 2024. Knockdown of HGH1 in breast cancer cell lines can inhibit the viability, invasion and migration of tumor cells. In Cell adhesion & migration, 19, 1-14. doi:10.1080/19336918.2024.2442349. https://pubmed.ncbi.nlm.nih.gov/39691959/
6. Zhang, Chenxin, E, Jifu, Yu, Enda. 2021. LncRNA CASC21 induces HGH1 to mediate colorectal cancer cell proliferation, migration, EMT and stemness. In RNA biology, 18, 369-381. doi:10.1080/15476286.2021.1950464. https://pubmed.ncbi.nlm.nih.gov/34375566/
7. Vakili, Hana, Jin, Yan, Cattini, Peter A. 2012. Negative regulation of human growth hormone gene expression by insulin is dependent on hypoxia-inducible factor binding in primary non-tumor pituitary cells. In The Journal of biological chemistry, 287, 33282-92. doi:. https://pubmed.ncbi.nlm.nih.gov/22833680/
8. Fulton, Melody D, Yama, Danielle J, Dahl, Ella, Johnson, Jill L. 2024. Hsp90 and cochaperones have two genetically distinct roles in regulating eEF2 function. In PLoS genetics, 20, e1011508. doi:10.1371/journal.pgen.1011508. https://pubmed.ncbi.nlm.nih.gov/39652595/
9. Thomsen, Louiza Bohn, Lichota, Jacek, Kim, Kwang Sik, Moos, Torben. 2011. Gene delivery by pullulan derivatives in brain capillary endothelial cells for protein secretion. In Journal of controlled release : official journal of the Controlled Release Society, 151, 45-50. doi:10.1016/j.jconrel.2011.01.002. https://pubmed.ncbi.nlm.nih.gov/21251935/