Grin3a-KO 基因敲除小鼠

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产品名称

Grin3a-KO 基因敲除小鼠

产品编号

S-KO-07366

品系全称

C57BL/6NCya-Grin3aem1/Cya

品系背景

C57BL/6NCya

品系编号

KOCMP-242443-Grin3a-B6N-VA

品系状态

使用本品系发表的文献需注明: Grin3a-KO 基因敲除小鼠 mice (Strain S-KO-07366) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
glutamate receptor ionotropic, NMDA3A
基因别称
6430537F04,A830097C19Rik,GluN3A,NMDAR-L,NMDAR3A,NR3A,mKIAA1973
染色体号
Chr 4 (Mouse)
转录本 ID
NCBI: NM_001276355 | Ensembl: ENSMUST00000093859
修饰方式
全身性基因敲除
靶向范围
Exon 2
敲除长度
~0.6 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1933206Mice homozygous for a disruption in this gene display increased current densities in some cerebrocortical neurons of the brain, increased levels of prepulse inhibition, and altered dendritic spine morphology. Otherwise, they display a normal phenotype.
Grin3a(也称为GluN3A或NR3A)是编码N-甲基-D-天冬氨酸受体(NMDAR)3A亚基的基因。NMDAR是离子型谷氨酸受体家族的重要成员,在神经系统中发挥着关键作用,参与突触传递、神经可塑性、学习和记忆等过程。Grin3a基因在哺乳动物大脑中表达,尤其在发育过程中对神经网络的成熟和稳定至关重要。Grin3a基因的表达模式随脑区域、皮质层和感觉模式的不同而有所差异,与脑成熟和功能分化的梯度相关。Grin3a基因在成年后仍保持表达,尤其是在杏仁核、中缝背核、联合皮层和高阶丘脑核等特定脑区[4]。

Grin3a基因的表达不仅在生理过程中发挥作用,还与多种疾病的发生发展相关。例如,研究表明,Grin3a基因的遗传变异与一些精神疾病有关。在抑郁症研究中,发现Grin3a基因的某些单核苷酸多态性(SNPs)与产后抑郁症的发生有关[1]。此外,Grin3a基因的变异还与精神分裂症有关,尽管在亚洲人群中没有发现明确的关联[5]。

Grin3a基因还与一些神经系统疾病相关。例如,研究发现,Grin3a基因的表达下调与阿尔茨海默病(AD)中tau蛋白异常聚集导致的神经功能障碍有关[7]。Grin3a基因的表达还与前列腺癌的预后相关,Grin3a基因的表达水平在筛状型前列腺癌中显著升高,提示Grin3a基因可能是筛状型前列腺癌的一个RNA生物标志物,与不良预后相关[6]。

除了在神经系统和精神疾病中的作用外,Grin3a基因还与一些其他疾病相关。例如,研究发现,Grin3a基因的变异与川崎病并发冠状动脉瘤的形成有关[2]。此外,Grin3a基因的变异还与心脏手术后谵妄的发生有关[3]。还有研究表明,Grin3a基因的变异可能与急性淋巴细胞白血病患儿接受糖皮质激素治疗后出现骨坏死有关[8]。此外,Grin3a基因的变异还与尼古丁依赖有关,Grin3a基因的某些SNPs与尼古丁依赖的严重程度相关[9]。

综上所述,Grin3a基因在神经系统和精神疾病中发挥着重要作用,其遗传变异与多种疾病的发生发展相关。Grin3a基因的研究有助于深入理解神经系统和精神疾病的发病机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Ping, Anqi, Yang, Mi, Xu, Shouyu, Wang, Saiying, Duan, Kaiming. 2023. Correlations between GRIN2B and GRIN3A gene polymorphisms and postpartum depressive symptoms in Chinese parturients undergoing cesarean section: A prospective cohort study. In Journal of psychosomatic research, 168, 111210. doi:10.1016/j.jpsychores.2023.111210. https://pubmed.ncbi.nlm.nih.gov/36898314/
2. Lin, Ying-Ju, Chang, Jeng-Sheng, Liu, Xiang, Chang, Li-Ching, Tsai, Fuu-Jen. 2013. Association between GRIN3A gene polymorphism in Kawasaki disease and coronary artery aneurysms in Taiwanese children. In PloS one, 8, e81384. doi:10.1371/journal.pone.0081384. https://pubmed.ncbi.nlm.nih.gov/24278430/
3. Kazmierski, Jakub, Sieruta, Monika, Banys, Andrzej, Liberski, Pawel, Kloszewska, Iwona. 2014. The assessment of the T102C polymorphism of the 5HT2a receptor gene, 3723G/A polymorphism of the NMDA receptor 3A subunit gene (GRIN3A) and 421C/A polymorphism of the NMDA receptor 2B subunit gene (GRIN2B) among cardiac surgery patients with and without delirium. In General hospital psychiatry, 36, 753-6. doi:10.1016/j.genhosppsych.2014.06.002. https://pubmed.ncbi.nlm.nih.gov/25041634/
4. Murillo, Alvaro, Navarro, Ana I, Puelles, Eduardo, Petros, Timothy J, Pérez-Otaño, Isabel. . Temporal Dynamics and Neuronal Specificity of Grin3a Expression in the Mouse Forebrain. In Cerebral cortex (New York, N.Y. : 1991), 31, 1914-1926. doi:10.1093/cercor/bhaa330. https://pubmed.ncbi.nlm.nih.gov/33290502/
5. Shen, Yu-Chih, Liao, Ding-Lieh, Chen, Jen-Yeu, Luu, Sy-Ueng, Chen, Chia-Hsiang. 2009. Exomic sequencing of the ionotropic glutamate receptor N-methyl-D-aspartate 3A gene (GRIN3A) reveals no association with schizophrenia. In Schizophrenia research, 114, 25-32. doi:10.1016/j.schres.2009.07.005. https://pubmed.ncbi.nlm.nih.gov/19665356/
6. Bogaard, Mari, Strømme, Jonas M, Kidd, Susanne G, Skotheim, Rolf I, Axcrona, Ulrika. 2024. GRIN3A: A biomarker associated with a cribriform pattern and poor prognosis in prostate cancer. In Neoplasia (New York, N.Y.), 55, 101023. doi:10.1016/j.neo.2024.101023. https://pubmed.ncbi.nlm.nih.gov/38944914/
7. Lee, Sang-Eun, Park, Soomin, Kang, Rian, Chang, Sunghoe, Park, Jong-Chan. 2024. Hippocampal tau-induced GRIN3A deficiency in Alzheimer's disease. In FEBS open bio, 14, 2059-2071. doi:10.1002/2211-5463.13904. https://pubmed.ncbi.nlm.nih.gov/39396906/
8. Zgheib, Nathalie K, El-Khoury, Habib, Maamari, Dimitri, Saab, Raya, Muwakkit, Samar A. 2021. A GRIN3A polymorphism may be associated with glucocorticoid-induced symptomatic osteonecrosis in children with acute lymphoblastic leukemia. In Personalized medicine, 18, 431-439. doi:10.2217/pme-2020-0167. https://pubmed.ncbi.nlm.nih.gov/34406079/
9. Chen, Jiali, Liu, Qiang, Fan, Rongli, Song, Guohua, Li, Ming D. 2019. Demonstration of critical role of GRIN3A in nicotine dependence through both genetic association and molecular functional studies. In Addiction biology, 25, e12718. doi:10.1111/adb.12718. https://pubmed.ncbi.nlm.nih.gov/30741440/