Lims2-KO 基因敲除小鼠

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产品名称

Lims2-KO 基因敲除小鼠

产品编号

S-KO-05873

品系全称

C57BL/6JCya-Lims2em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-225341-Lims2-B6J-VA

品系状态

使用本品系发表的文献需注明: Lims2-KO 基因敲除小鼠 mice (Strain S-KO-05873) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
LIM and senescent cell antigen like domains 2
基因别称
PINCH2
染色体号
Chr 18 (Mouse)
转录本 ID
NCBI: NM_144862.4 | Ensembl: ENSMUST00000025254
修饰方式
全身性基因敲除
靶向范围
Exon 2
敲除长度
~160 bp
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:2385067Homozygous null mice are viable and fertile with no gross abnormalities. Mice homozygous for a different targeted allele exhibit decreased fractional shortening and increased area affected following myocardial infarct.
Lims2,也称为PINCH2,是LIM锌指结构域家族成员之一。LIM锌指结构域是一种蛋白质结构域,参与蛋白质之间的相互作用和细胞信号传导。Lims2在细胞迁移和粘附中起着重要作用,特别是在肌细胞和心肌细胞中。此外,Lims2还与一些疾病的发生和发展有关,例如胃癌症和动脉粥样硬化。

在胃癌症中,Lims2的表达水平显著降低,这与Lims2启动子区域的CpG岛高甲基化有关[5]。Lims2的失活可能促进肿瘤的侵袭和转移,因此Lims2可能是一个有用的分子生物标志物和潜在的治疗靶点。

在动脉粥样硬化中,Lims2的表达受到血液剪切应力的影响,血液剪切应力是血管内皮细胞受到的一种机械力[4]。当血液剪切应力紊乱时,Lims2的表达水平降低,导致内皮细胞炎症,促进动脉粥样硬化的发生和发展。

此外,Lims2还与一些疾病的发生和发展有关。例如,在肺动脉高压(PAH)患者中,Lims2的启动子区域甲基化水平升高,与PAH的发病机制和预后相关[6]。在肝细胞癌(HCC)中,Lims2的表达水平与患者的预后和治疗反应相关[8]。

综上所述,Lims2是一种重要的基因,在细胞迁移、粘附和疾病发生中发挥着重要作用。Lims2的表达受到多种因素的调节,包括DNA甲基化和血液剪切应力。Lims2的研究有助于深入理解细胞信号传导和疾病发生机制,为疾病的治疗和预防提供新的思路和策略[1,2,3,4,5,6,7,8]。

参考文献:
1. Megarbane, Andre, Bizzari, Sami, Deepthi, Asha, Delague, Valérie, Urtizberea, J Andoni. . A 20-year Clinical and Genetic Neuromuscular Cohort Analysis in Lebanon: An International Effort. In Journal of neuromuscular diseases, 9, 193-210. doi:10.3233/JND-210652. https://pubmed.ncbi.nlm.nih.gov/34602496/
2. Wang, Wen-Juan, Huang, Rong, Zheng, Tao, Ouyang, Fengxiu, Luo, Zhong-Cheng. 2022. Genome-Wide Placental Gene Methylations in Gestational Diabetes Mellitus, Fetal Growth and Metabolic Health Biomarkers in Cord Blood. In Frontiers in endocrinology, 13, 875180. doi:10.3389/fendo.2022.875180. https://pubmed.ncbi.nlm.nih.gov/35721735/
3. Sarıkaya Uzan, Gamze, Yılmaz Uzman, Ceren, Çinleti, Tayfun, Hız Kurul, Semra, Yiş, Uluç. 2023. Molecular Diagnosis of Limb-Girdle Muscular Dystrophy Using Next-Generation Sequencing Panels. In Molecular syndromology, 15, 14-21. doi:10.1159/000533976. https://pubmed.ncbi.nlm.nih.gov/38357257/
4. Wang, Junyao, Zhang, Shiyanjin. 2020. Fluid shear stress modulates endothelial inflammation by targeting LIMS2. In Experimental biology and medicine (Maywood, N.J.), 245, 1656-1663. doi:10.1177/1535370220943837. https://pubmed.ncbi.nlm.nih.gov/32752897/
5. Kim, Seung-Kyoon, Jang, Hae-Ran, Kim, Jeong-Hwan, Yoo, Hyang-Sook, Kim, Yong Sung. 2006. The epigenetic silencing of LIMS2 in gastric cancer and its inhibitory effect on cell migration. In Biochemical and biophysical research communications, 349, 1032-40. doi:. https://pubmed.ncbi.nlm.nih.gov/16959213/
6. Benincasa, Giuditta, Maron, Bradley A, Affinito, Ornella, Loscalzo, Joseph, Napoli, Claudio. 2022. Association Between Circulating CD4+ T Cell Methylation Signatures of Network-Oriented SOCS3 Gene and Hemodynamics in Patients Suffering Pulmonary Arterial Hypertension. In Journal of cardiovascular translational research, 16, 17-30. doi:10.1007/s12265-022-10294-1. https://pubmed.ncbi.nlm.nih.gov/35960497/
7. Song, Tianyi, Spillmann, Dorothe. 2019. Transcriptomic analysis reveals cell apoptotic signature modified by heparanase in melanoma cells. In Journal of cellular and molecular medicine, 23, 4559-4568. doi:10.1111/jcmm.14349. https://pubmed.ncbi.nlm.nih.gov/31044520/
8. Chen, Lei, Zhang, Dafang, Zheng, Shengmin, Li, Xinyu, Gao, Pengji. 2022. Stemness analysis in hepatocellular carcinoma identifies an extracellular matrix gene-related signature associated with prognosis and therapy response. In Frontiers in genetics, 13, 959834. doi:10.3389/fgene.2022.959834. https://pubmed.ncbi.nlm.nih.gov/36110210/