Tph1-KO 基因敲除小鼠

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产品名称

Tph1-KO 基因敲除小鼠

产品编号

S-KO-05540

品系全称

C57BL/6NCya-Tph1em1/Cya

品系背景

C57BL/6NCya

品系编号

KOCMP-21990-Tph1-B6N-VA

品系状态

使用本品系发表的文献需注明: Tph1-KO 基因敲除小鼠 mice (Strain S-KO-05540) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
tryptophan hydroxylase 1
基因别称
Tph
染色体号
Chr 7 (Mouse)
转录本 ID
NCBI: NM_009414 | Ensembl: ENSMUST00000049298
修饰方式
全身性基因敲除
靶向范围
Exon 3~10
敲除长度
~12.2 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:98796Mice homozygous for one null allele display no gross behavioral abnormalities. Mice homozygous for a second null allele display fatigue, breathing difficulties, progressive pallor, and impaired cardiac function.

发表文献

Molecular Cell
2024-02-14
A GAPDH serotonylation system couples CD8+ T cell glycolytic metabolism to antitumor immunity
1
Tph1,也称为色氨酸羟化酶1(Tryptophan hydroxylase 1),是5-羟色胺(5-HT)合成途径中的限速酶。5-HT是一种重要的神经递质,在情绪调节、认知功能、睡眠和消化系统中扮演关键角色。Tph1的活性受到多种因素的调控,包括基因表达、转录后修饰和环境因素等。

Tph1基因多态性已被证明与多种疾病的发生和进展相关。例如,一项研究发现,Tph1基因的rs211105多态性与急性胰腺炎患者血液中色氨酸羟化酶1浓度相关[1]。此外,Tph1基因的A218C多态性还与精神分裂症和自杀行为的易感性相关[2,5]。一项针对肠易激综合症的研究发现,Tph1基因的rs211105多态性会影响患者的腹部症状和生活质量[3]。此外,Tph1基因的表达和活性还与慢性肾脏病、肺部高血压等疾病的发生和发展相关[4,6,7]。

肠道微生物群也被证明可以影响Tph1的表达和活性。一项研究发现,肠道微生物群通过短链脂肪酸(SCFA)影响肠嗜铬细胞(EC)中的Tph1表达,进而调节肠道5-HT的产生和稳态[8]。

综上所述,Tph1在多种生物学过程中发挥着重要作用,其表达和活性受到多种因素的调控。Tph1基因多态性与多种疾病的发生和进展相关,包括急性胰腺炎、精神分裂症、肠易激综合症等。肠道微生物群也可以影响Tph1的表达和活性。因此,深入研究Tph1的生物学功能和调控机制,有助于我们更好地理解相关疾病的发病机制,为疾病的诊断、治疗和预防提供新的思路和策略。

参考文献:
1. Snarska, Jadwiga, Fiedorowicz, Ewa, Rozmus, Dominika, Kostyra, Elżbieta, Cieślińska, Anna. 2021. TPH1 gene polymorphism rs211105 is associated with serotonin and tryptophan hydroxylase 1 concentrations in acute pancreatitis patients. In BMC gastroenterology, 21, 426. doi:10.1186/s12876-021-02012-z. https://pubmed.ncbi.nlm.nih.gov/34772352/
2. Saetre, Peter, Lundmark, Per, Wang, August, Terenius, Lars, Jönsson, Erik G. . The tryptophan hydroxylase 1 (TPH1) gene, schizophrenia susceptibility, and suicidal behavior: a multi-centre case-control study and meta-analysis. In American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, 153B, 387-396. doi:10.1002/ajmg.b.30991. https://pubmed.ncbi.nlm.nih.gov/19526457/
3. Katsumata, Ryo, Shiotani, Akiko, Murao, Takahisa, Matsumoto, Hiroshi, Haruma, Ken. 2018. The TPH1 rs211105 gene polymorphism affects abdominal symptoms and quality of life of diarrhea-predominant irritable bowel syndrome. In Journal of clinical biochemistry and nutrition, 62, 270-276. doi:10.3164/jcbn.17-76. https://pubmed.ncbi.nlm.nih.gov/29892168/
4. Morecroft, Ian, White, Katie, Caruso, Paola, Baker, Andrew H, Maclean, Margaret R. 2012. Gene therapy by targeted adenovirus-mediated knockdown of pulmonary endothelial Tph1 attenuates hypoxia-induced pulmonary hypertension. In Molecular therapy : the journal of the American Society of Gene Therapy, 20, 1516-28. doi:10.1038/mt.2012.70. https://pubmed.ncbi.nlm.nih.gov/22525513/
5. Genis-Mendoza, Alma Delia, Hernández-Díaz, Yazmín, González-Castro, Thelma Beatriz, Ramos-Méndez, Miguel Ángel, Nicolini, Humberto. 2022. Association between TPH1 polymorphisms and the risk of suicide behavior: An updated meta-analysis of 18,398 individuals. In Frontiers in psychiatry, 13, 932135. doi:10.3389/fpsyt.2022.932135. https://pubmed.ncbi.nlm.nih.gov/35928776/
6. Chen, Dan-Qian, Cao, Gang, Chen, Hua, Guo, Yan, Zhao, Ying-Yong. 2019. Identification of serum metabolites associating with chronic kidney disease progression and anti-fibrotic effect of 5-methoxytryptophan. In Nature communications, 10, 1476. doi:10.1038/s41467-019-09329-0. https://pubmed.ncbi.nlm.nih.gov/30931940/
7. Grozić, Aleksandra, Coker, Keaton, Dussik, Christopher M, Sandrin, Todd R, Jurutka, Peter W. 2022. Identification of putative transcriptomic biomarkers in irritable bowel syndrome (IBS): Differential gene expression and regulation of TPH1 and SERT by vitamin D. In PloS one, 17, e0275683. doi:10.1371/journal.pone.0275683. https://pubmed.ncbi.nlm.nih.gov/36264926/
8. Reigstad, Christopher S, Salmonson, Charles E, Rainey, John F, Farrugia, Gianrico, Kashyap, Purna C. 2014. Gut microbes promote colonic serotonin production through an effect of short-chain fatty acids on enterochromaffin cells. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 29, 1395-403. doi:10.1096/fj.14-259598. https://pubmed.ncbi.nlm.nih.gov/25550456/

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