Arhgef11-KO 基因敲除小鼠

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产品名称

Arhgef11-KO 基因敲除小鼠

产品编号

S-KO-04934

品系全称

C57BL/6JCya-Arhgef11em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-213498-Arhgef11-B6J-VB

品系状态

使用本品系发表的文献需注明: Arhgef11-KO 基因敲除小鼠 mice (Strain S-KO-04934) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
Rho guanine nucleotide exchange factor 11
基因别称
PDZ-RhoGEF,Prg,RhoGEF,mKIAA0380
染色体号
Chr 3 (Mouse)
转录本 ID
NCBI: NM_001003912 | Ensembl: ENSMUST00000039476
修饰方式
全身性基因敲除
靶向范围
Exon 2~3
敲除长度
~2.2 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:2441869Mice homozygous for a knock-out allele exhibit no obvious phenotype.
ARHGEF11,也称为Rho guanine nucleotide exchange factor 11,是一种编码Rho鸟嘌呤核苷酸交换因子11的基因。Rho鸟嘌呤核苷酸交换因子是一类参与调节Rho GTPase活性的蛋白质,而Rho GTPase则参与调控多种细胞过程,包括细胞骨架组织、细胞迁移、细胞粘附和信号转导等。ARHGEF11在多种生物学过程中发挥作用,包括胰岛素信号传导、肿瘤发生发展、肾脏损伤和神经母细胞瘤等。

在胰岛素信号传导方面,ARHGEF11的变异与胰岛素抵抗和2型糖尿病的风险增加有关[1][5][6]。在肿瘤发生发展方面,ARHGEF11在肝细胞癌中表达上调,并通过激活β-catenin通路促进肿瘤增殖和转移[2]。在肾脏损伤方面,ARHGEF11的变异与肾小球滤过率降低和肾损伤相关[4][7]。在神经母细胞瘤方面,ARHGEF11的表达上调与不良预后相关,并可通过抑制YWHAH表达激活PI3K/AKT信号通路促进肿瘤细胞活性[3]。

此外,ARHGEF11的剪接变异体对上皮紧密连接的完整性也具有重要影响。ARHGEF11的剪接变异体在维持上皮紧密连接和细胞骨架组织方面发挥重要作用,而其剪接变异体的缺失或减少则会导致上皮屏障功能缺陷[3]。

综上所述,ARHGEF11是一种重要的基因,在多种生物学过程中发挥作用,包括胰岛素信号传导、肿瘤发生发展、肾脏损伤和神经母细胞瘤等。ARHGEF11的变异和剪接变异体在多种疾病的发生发展中起着重要作用,为进一步研究疾病发生机制和开发新的治疗方法提供了重要的参考和依据。

参考文献:
1. Ma, Lijun, Hanson, Robert L, Que, Lorem N, Shuldiner, Alan R, Baier, Leslie J. 2007. Variants in ARHGEF11, a candidate gene for the linkage to type 2 diabetes on chromosome 1q, are nominally associated with insulin resistance and type 2 diabetes in Pima Indians. In Diabetes, 56, 1454-9. doi:. https://pubmed.ncbi.nlm.nih.gov/17287471/
2. Du, Jinpeng, Zhu, Zexin, Xu, Lin, Yuan, Kefei, Zeng, Yong. 2020. ARHGEF11 promotes proliferation and epithelial-mesenchymal transition of hepatocellular carcinoma through activation of β-catenin pathway. In Aging, 12, 20235-20253. doi:10.18632/aging.103772. https://pubmed.ncbi.nlm.nih.gov/33122451/
3. Lee, SungKyoung, Cieply, Benjamin, Yang, Yueqin, Chan, Patricia, Carstens, Russ P. . Esrp1-Regulated Splicing of Arhgef11 Isoforms Is Required for Epithelial Tight Junction Integrity. In Cell reports, 25, 2417-2430.e5. doi:10.1016/j.celrep.2018.10.097. https://pubmed.ncbi.nlm.nih.gov/30485810/
4. Jia, Zhen, Johnson, Ashley C, Wang, Xuexiang, Kyle, Patrick B, Garrett, Michael R. 2015. Allelic Variants in Arhgef11 via the Rho-Rock Pathway Are Linked to Epithelial-Mesenchymal Transition and Contributes to Kidney Injury in the Dahl Salt-Sensitive Rat. In PloS one, 10, e0132553. doi:10.1371/journal.pone.0132553. https://pubmed.ncbi.nlm.nih.gov/26172442/
5. Fu, Mao, Sabra, Mona M, Damcott, Coleen, Baier, Leslie J, Shuldiner, Alan R. 2007. Evidence that Rho guanine nucleotide exchange factor 11 (ARHGEF11) on 1q21 is a type 2 diabetes susceptibility gene in the Old Order Amish. In Diabetes, 56, 1363-8. doi:. https://pubmed.ncbi.nlm.nih.gov/17369523/
6. Liu, Jing, Chen, Xiaohui, Guo, Qian, Zhang, Xiaohui, Zhang, Shulan. 2011. Association of ARHGEF11 R1467H polymorphism with risk for type 2 diabetes mellitus and insulin resistance in Chinese population. In Molecular biology reports, 38, 2499-505. doi:10.1007/s11033-010-0387-5. https://pubmed.ncbi.nlm.nih.gov/21210224/
7. Williams, Jan M, Johnson, Ashley C, Stelloh, Cary, Roman, Richard J, Garrett, Michael R. 2012. Genetic variants in Arhgef11 are associated with kidney injury in the Dahl salt-sensitive rat. In Hypertension (Dallas, Tex. : 1979), 60, 1157-68. doi:10.1161/HYPERTENSIONAHA.112.199240. https://pubmed.ncbi.nlm.nih.gov/22987919/