Rab19-KO 基因敲除小鼠

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产品名称

Rab19-KO 基因敲除小鼠

产品编号

S-KO-03973

品系全称

C57BL/6NCya-Rab19em1/Cya

品系背景

C57BL/6NCya

品系编号

KOCMP-19331-Rab19-B6N-VA

品系状态

使用本品系发表的文献需注明: Rab19-KO 基因敲除小鼠 mice (Strain S-KO-03973) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
RAB19, member RAS oncogene family
基因别称
-
染色体号
Chr 6 (Mouse)
转录本 ID
NCBI: NM_011226 | Ensembl: ENSMUST00000031986
修饰方式
全身性基因敲除
靶向范围
Exon 2~4
敲除长度
~6.5 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
Rab19是一种新发现的、组织特异性表达的Rab小GTP酶家族成员。通过快速扩增cDNA末端(RACE)克隆方法,研究人员成功分离了编码Rab19的cDNA克隆。Northern blot分析显示,rab19 mRNA在肠道、肺和脾脏中高水平表达,在肾脏中低水平表达,而在肝脏、大脑、心脏和NIH 3T3成纤维细胞中几乎检测不到。这表明Rab19可能是一种新的组织或细胞类型特异性小GTP酶[1]。

Rab19在多种生物学过程中发挥作用。例如,在口腔鳞状细胞癌(OSCC)中,Rab19是免疫评分相关风险签名的一部分,与患者的预后分层和生存状态相关。此外,Rab19还参与了肠道Slit/Robo信号通路的调节,影响肠内分泌细胞的分化。在胃癌中,LINC01320通过miR495-5p/RAB19轴促进肿瘤的增殖、迁移和侵袭。M6A修饰的LINC01320通过METTL14介导的m6A修饰上调,进而影响RAB19的表达。在胃腺癌中,RAB19与患者的预后相关,并可能参与免疫浸润的调节。在结直肠癌中,RAB19表达上调,与肿瘤生长和细胞代谢相关。此外,Rab19还与RAB41密切相关,RAB41是一种新的RAB基因,与RAB19具有高度相似性,在多种组织中广泛表达[2][3][4][5][6][7][8]。

综上所述,Rab19是一种重要的Rab小GTP酶家族成员,参与多种生物学过程,包括肿瘤的发生和发展、细胞分化、信号通路调节等。Rab19的研究有助于深入理解Rab蛋白在细胞功能调控中的作用,为相关疾病的诊断、治疗和预防提供新的思路和策略。

参考文献:
1. Lütcke, A, Olkkonen, V M, Dupree, P, Simons, K, Zerial, M. . Isolation of a murine cDNA clone encoding Rab19, a novel tissue-specific small GTPase. In Gene, 155, 257-60. doi:. https://pubmed.ncbi.nlm.nih.gov/7721101/
2. Huang, Su-Ning, Li, Guo-Sheng, Zhou, Xian-Guo, Tang, Xiao-Zhun, Rong, Min-Hua. 2020. Identification of an Immune Score-Based Gene Panel with Prognostic Power for Oral Squamous Cell Carcinoma. In Medical science monitor : international medical journal of experimental and clinical research, 26, e922854. doi:10.12659/MSM.922854. https://pubmed.ncbi.nlm.nih.gov/32529991/
3. Nagy, Péter, Szatmári, Zsuzsanna, Sándor, Gyöngyvér O, Hegedűs, Krisztina, Juhász, Gábor. 2017. Drosophila Atg16 promotes enteroendocrine cell differentiation via regulation of intestinal Slit/Robo signaling. In Development (Cambridge, England), 144, 3990-4001. doi:10.1242/dev.147033. https://pubmed.ncbi.nlm.nih.gov/28982685/
4. Hu, Naijun, Ji, Hong. . N6-methyladenosine (m6A)-mediated up-regulation of long noncoding RNA LINC01320 promotes the proliferation, migration, and invasion of gastric cancer via miR495-5p/RAB19 axis. In Bioengineered, 12, 4081-4091. doi:10.1080/21655979.2021.1953210. https://pubmed.ncbi.nlm.nih.gov/34288797/
5. Luo, Na, Fu, Min, Zhang, Yiling, Li, Qianxia, Hu, Guangyuan. 2022. Prognostic Role of M6A-Associated Immune Genes and Cluster-Related Tumor Microenvironment Analysis: A Multi-Omics Practice in Stomach Adenocarcinoma. In Frontiers in cell and developmental biology, 10, 935135. doi:10.3389/fcell.2022.935135. https://pubmed.ncbi.nlm.nih.gov/35859893/
6. Jiang, Xuefei, Yang, Lanlan, Gao, Qianling, Ye, Shubiao, Yang, Zihuan. 2022. The Role of RAB GTPases and Its Potential in Predicting Immunotherapy Response and Prognosis in Colorectal Cancer. In Frontiers in genetics, 13, 828373. doi:10.3389/fgene.2022.828373. https://pubmed.ncbi.nlm.nih.gov/35154286/
7. Schluth-Bolard, Caroline, Labalme, Audrey, Cordier, Marie-Pierre, Edery, Patrick, Sanlaville, Damien. 2013. Breakpoint mapping by next generation sequencing reveals causative gene disruption in patients carrying apparently balanced chromosome rearrangements with intellectual deficiency and/or congenital malformations. In Journal of medical genetics, 50, 144-50. doi:10.1136/jmedgenet-2012-101351. https://pubmed.ncbi.nlm.nih.gov/23315544/
8. Guo, Jin-Hu, Chen, Li, Chen, Shuai, Yu, Long, Zhao, Shou-Yuan. . Isolation, expression pattern of a novel human RAB gene RAB41 and characterization of its intronless homolog RAB41P. In DNA sequence : the journal of DNA sequencing and mapping, 14, 431-5. doi:. https://pubmed.ncbi.nlm.nih.gov/15018353/