Ephx2-KO 基因敲除小鼠

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产品名称

Ephx2-KO 基因敲除小鼠

产品编号

S-KO-01907

品系全称

C57BL/6NCya-Ephx2em1/Cya

品系背景

C57BL/6NCya

品系编号

KOCMP-13850-Ephx2-B6N-VA

品系状态

使用本品系发表的文献需注明: Ephx2-KO 基因敲除小鼠 mice (Strain S-KO-01907) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
epoxide hydrolase 2, cytoplasmic
基因别称
CEH,Eph2,SEH,sEP
染色体号
Chr 14 (Mouse)
转录本 ID
NCBI: NM_007940 | Ensembl: ENSMUST00000070515
修饰方式
全身性基因敲除
靶向范围
Exon 2~5
敲除长度
~10.8 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:99500Males homozygous for a targeted null mutation display a significant reduction in blood pressure both in the absence and presence of dietary salt loading. Both sexes exhibit altered arachidonic acid metabolism and reduced renal formation of epoxyeicosatrienoic and dihydroxyeicosatrienoic acids.

发表文献

International Journal of Biological Sciences
2022-12-31
Direct targeting of sEH with alisol B alleviated the apoptosis, inflammation, and oxidative stress in cisplatin-induced acute kidney injury
1
EPHX2,也称为环氧合酶2,是一种编码可溶性表儿茶酚酶(soluble epoxide hydrolase,sEH)的基因。sEH是一种重要的生物活性脂质代谢酶,主要负责降解环氧合酶三烯酸(EETs),EETs是一种具有血管舒张、降胆固醇和抗炎作用的保护因子。EPHX2的变异已被发现与多种心血管疾病(CVDs)相关,包括冠状动脉疾病、高血压和房颤复发[3]。此外,EPHX2还可能参与胆固醇代谢和神经递质调节[4]。

在神经系统中,EPHX2的表达和功能与阿尔茨海默病(AD)的发病机制有关。研究表明,EPHX2可能通过影响整个海马体积来介导AD的病理发生[1]。此外,EPHX2的基因多态性与汉族人群中高血压的易感性相关[2],这可能进一步影响AD的发病风险。

在心血管系统中,EPHX2的基因变异与氧化低密度脂蛋白(ox-LDL)和死亡率相关。研究发现,EPHX2基因的rs11780592位点多态性与ox-LDL、颈动脉内膜中层厚度(cIMT)、蛋白尿和高血压相关,并且与糖尿病慢性肾病(CKD)患者的全因死亡率显著相关[7]。

此外,EPHX2的表达和功能还与胆固醇代谢和神经递质调节有关。研究表明,EPHX2的表达下调与肝细胞癌(HCC)的发展相关,EPHX2可能成为HCC的潜在治疗靶点[6]。同时,EPHX2的表达受高糖条件下的Sp1转录因子抑制,这可能导致糖尿病等代谢性疾病的发生[5]。

综上所述,EPHX2基因在多种疾病中发挥重要作用,包括AD、高血压、HCC和糖尿病等。EPHX2的基因变异和表达水平可能影响疾病的易感性和预后,为疾病的预防和治疗提供新的思路和策略。

参考文献:
1. Su, Wei-Ming, Gu, Xiao-Jing, Dou, Meng, Wang, Yi, Chen, Yong-Ping. 2023. Systematic druggable genome-wide Mendelian randomisation identifies therapeutic targets for Alzheimer's disease. In Journal of neurology, neurosurgery, and psychiatry, 94, 954-961. doi:10.1136/jnnp-2023-331142. https://pubmed.ncbi.nlm.nih.gov/37349091/
2. Zhu, X L, Wang, L, Wang, Z, Zhang, W Q, Ma, M M. 2015. Relationship between EPHX2 gene polymorphisms and essential hypertension in Uygur, Kazakh, and Han. In Genetics and molecular research : GMR, 14, 3474-80. doi:10.4238/2015.April.15.11. https://pubmed.ncbi.nlm.nih.gov/25966114/
3. Zhu, Xiaoming, Li, Yuxin, Yu, Tingting, Li, Sen, Chen, Mulei. 2022. A hypothesis-driven study to comprehensively investigate the association between genetic polymorphisms in EPHX2 gene and cardiovascular diseases: Findings from the UK Biobank. In Gene, 822, 146340. doi:10.1016/j.gene.2022.146340. https://pubmed.ncbi.nlm.nih.gov/35183688/
4. Scott-Van Zeeland, A A, Bloss, C S, Tewhey, R, Kaye, W, Schork, N J. 2013. Evidence for the role of EPHX2 gene variants in anorexia nervosa. In Molecular psychiatry, 19, 724-32. doi:10.1038/mp.2013.91. https://pubmed.ncbi.nlm.nih.gov/23999524/
5. Oguro, Ami, Oida, Shoko, Imaoka, Susumu. 2015. Down-regulation of EPHX2 gene transcription by Sp1 under high-glucose conditions. In The Biochemical journal, 470, 281-91. doi:10.1042/BJ20150397. https://pubmed.ncbi.nlm.nih.gov/26341485/
6. Zhan, Ke, Bai, Yang, Liao, Shengtao, Qiu, Liewang, Mei, Zhechuan. 2021. Identification and validation of EPHX2 as a prognostic biomarker in hepatocellular carcinoma. In Molecular medicine reports, 24, . doi:10.3892/mmr.2021.12289. https://pubmed.ncbi.nlm.nih.gov/34278494/
7. Roumeliotis, Stefanos, Roumeliotis, Athanasios, Stamou, Aikaterini, Georgitsi, Marianthi, Liakopoulos, Vassilios. 2021. Association of rs11780592 Polymorphism in the Human Soluble Epoxide Hydrolase Gene (EPHX2) with Oxidized LDL and Mortality in Patients with Diabetic Chronic Kidney Disease. In Oxidative medicine and cellular longevity, 2021, 8817502. doi:10.1155/2021/8817502. https://pubmed.ncbi.nlm.nih.gov/34040693/

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