Prkg1-flox 基因敲除小鼠

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产品名称

Prkg1-flox 基因敲除小鼠

产品编号

S-CKO-19115

品系全称

C57BL/6JCya-Prkg1em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-19091-Prkg1-B6J-VB

品系状态

使用本品系发表的文献需注明: Prkg1-flox 基因敲除小鼠 mice (Strain S-CKO-19115) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
protein kinase, cGMP-dependent, type I
基因别称
CGKI,Gm19690,Prkg1b,Prkgr1b
染色体号
Chr 19 (Mouse)
转录本 ID
NCBI: NM_011160 | Ensembl: ENSMUST00000073581
修饰方式
条件性基因敲除
靶向范围
Exon 10
敲除长度
~1.5 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:108174Mutant mice exhibit abnormal smooth muscle function and penile erectile deficiency. Conditional disruption in the hippocampus results in impaired LTP. Mice homozygous for a transposon induced allele exhibit postnatal lethality.
PRKG1,也称为蛋白激酶G1,是一种cGMP依赖性蛋白激酶。cGMP是细胞内第二信使,参与多种生物学过程,包括平滑肌松弛、神经元信号传导、细胞增殖和分化。PRKG1是cGMP信号传导途径中的关键酶,它通过磷酸化靶蛋白来调节这些过程。PRKG1有两种同型异构体,即PRKG1α和PRKG1β,它们在组织分布和功能上有所不同。

PRKG1在多种疾病中发挥重要作用。例如,研究表明,PRKG1基因的某些单核苷酸多态性(SNPs)与儿童哮喘的易感性相关[1]。此外,PRKG1基因的变异还与牛奶脂肪酸含量有关,这为提高牛奶品质提供了候选分子标记[2][5]。PRKG1基因的下调与贲门失弛缓症的发生相关,这表明PRKG1基因可能参与调控食管平滑肌的收缩和松弛[3]。此外,PRKG1基因的变异还与人类自我调节和觅食行为相关[4][7]。

研究还发现,PRKG1基因的变异与遗传性胸主动脉疾病的易感性相关[6]。此外,PRKG1基因的表达还受miRNA的调控,例如miR-200c-3p和miR-20a,它们通过靶向PRKG1基因的3'-非翻译区或编码区来调节其表达[3][8]。

综上所述,PRKG1基因是一种重要的cGMP依赖性蛋白激酶,参与调控多种生物学过程。PRKG1基因的变异与多种疾病的发生和进展相关,包括哮喘、牛奶品质、贲门失弛缓症、遗传性胸主动脉疾病和觅食行为。此外,PRKG1基因的表达还受miRNA的调控。因此,深入研究PRKG1基因的功能和调控机制,有助于揭示相关疾病的发病机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Liu, Jun, Wei, Bing, Zhang, Yuxuan, You, Yuan, Zhi, Yanjie. 2024. Association between PRKG1 gene and gene-environment interactions with pediatric asthma. In The Journal of asthma : official journal of the Association for the Care of Asthma, 61, 754-761. doi:10.1080/02770903.2024.2303763. https://pubmed.ncbi.nlm.nih.gov/38193459/
2. Feng, Fen, Yang, Guowu, Ma, Xiaoyong, Zhandui, Pingcuo, Liang, Chunnian. 2024. Polymorphisms within the PRKG1 Gene of Gannan Yaks and Their Association with Milk Quality Characteristics. In Foods (Basel, Switzerland), 13, . doi:10.3390/foods13121913. https://pubmed.ncbi.nlm.nih.gov/38928854/
3. Micale, Lucia, Fusco, Carmela, Nardella, Grazia, Perri, Francesco, Latiano, Anna. 2022. Downexpression of miR-200c-3p Contributes to Achalasia Disease by Targeting the PRKG1 Gene. In International journal of molecular sciences, 24, . doi:10.3390/ijms24010668. https://pubmed.ncbi.nlm.nih.gov/36614110/
4. Struk, Andriy A, Mugon, Jhotisha, Huston, Andrea, Sokolowski, Marla B, Danckert, James. 2019. Self-regulation and the foraging gene (PRKG1) in humans. In Proceedings of the National Academy of Sciences of the United States of America, 116, 4434-4439. doi:10.1073/pnas.1809924116. https://pubmed.ncbi.nlm.nih.gov/30782798/
5. Shi, Lijun, Lv, Xiaoqing, Liu, Lin, Han, Bo, Sun, Dongxiao. 2019. A post-GWAS confirming effects of PRKG1 gene on milk fatty acids in a Chinese Holstein dairy population. In BMC genetics, 20, 53. doi:10.1186/s12863-019-0755-7. https://pubmed.ncbi.nlm.nih.gov/31269900/
6. Overwater, Eline, Marsili, Luisa, Baars, Marieke J H, Houweling, Arjan C, Maugeri, Alessandra. 2018. Results of next-generation sequencing gene panel diagnostics including copy-number variation analysis in 810 patients suspected of heritable thoracic aortic disorders. In Human mutation, 39, 1173-1192. doi:10.1002/humu.23565. https://pubmed.ncbi.nlm.nih.gov/29907982/
7. Sokolowski, H Moriah, Vasquez, Oscar E, Unternaehrer, Eva, Fleming, Alison S, Sokolowski, Marla B. 2016. The Drosophila foraging gene human orthologue PRKG1 predicts individual differences in the effects of early adversity on maternal sensitivity. In Cognitive development, 42, 62-73. doi:10.1016/j.cogdev.2016.11.001. https://pubmed.ncbi.nlm.nih.gov/28827895/
8. Zeng, Yan, Pan, Yanping, Liu, Hongtai, Raj, J Usha, Gou, Deming. 2014. MiR-20a regulates the PRKG1 gene by targeting its coding region in pulmonary arterial smooth muscle cells. In FEBS letters, 588, 4677-85. doi:10.1016/j.febslet.2014.10.040. https://pubmed.ncbi.nlm.nih.gov/25447536/