Hapln1-flox 基因敲除小鼠

下单100%中奖,最高可得千元京东卡
复苏/繁育服务
产品名称

Hapln1-flox 基因敲除小鼠

产品编号

S-CKO-18804

品系全称

C57BL/6JCya-Hapln1em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-12950-Hapln1-B6J-VB

品系状态

使用本品系发表的文献需注明: Hapln1-flox 基因敲除小鼠 mice (Strain S-CKO-18804) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
hyaluronan and proteoglycan link protein 1
基因别称
CLP,Crtl1,Crtl1l,LP,LP-1
染色体号
Chr 13 (Mouse)
转录本 ID
NCBI: NM_013500 | Ensembl: ENSMUST00000022108
修饰方式
条件性基因敲除
靶向范围
Exon 3~4
敲除长度
~4.8 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1337006Homozygotes for a targeted null mutation exhibit defects in cartilage development and delayed bone formation with short limbs and craniofacial anomalies. Mutants usually die as neonates due to respiratory failure, but some survive and develop dwarfism.
HAPLN1,也称为透明质酸和蛋白聚糖连接蛋白1,是一种重要的细胞外基质(ECM)蛋白。HAPLN1的主要功能是维持ECM分子(如透明质酸和蛋白聚糖)的聚集和结合活性,从而稳定ECM的大分子结构。HAPLN1在多种组织和疾病中发挥着重要作用,包括癌症、骨关节炎、心脏衰竭、骨质疏松和脊髓退行性变等[5,7,8]。

在癌症方面,HAPLN1的表达与多种癌症的进展和预后密切相关。研究发现,HAPLN1在胰腺癌、胃癌和骨髓瘤等多种癌症中表达上调,并促进肿瘤细胞的迁移、侵袭和免疫调节。例如,在胰腺癌中,HAPLN1的表达与基底型胰腺导管腺癌亚型相关,并预示着患者的不良预后[1]。在胃癌中,HAPLN1的表达与肿瘤的侵袭性和患者的生存率相关[2]。在骨髓瘤中,HAPLN1的表达与肿瘤细胞对多种治疗药物的耐药性相关[4,6]。这些研究表明,HAPLN1可能是癌症治疗和预后评估的潜在靶点和生物标志物。

除了癌症,HAPLN1还与骨关节炎的发病机制相关。研究发现,HAPLN1的表达在骨关节炎患者的滑膜组织中升高,并促进滑膜细胞的增殖和炎症反应[3]。此外,HAPLN1还与心脏衰竭、骨质疏松和脊髓退行性变等相关,表明其在多种疾病的发生发展中发挥着重要作用。

综上所述,HAPLN1是一种重要的细胞外基质蛋白,参与维持ECM的结构和功能。HAPLN1在多种疾病中表达上调,并促进肿瘤细胞的迁移、侵袭和免疫调节,以及骨关节炎、心脏衰竭、骨质疏松和脊髓退行性变等疾病的发生发展。HAPLN1的研究有助于深入理解细胞外基质在疾病发生发展中的作用机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Wiedmann, Lena, De Angelis Rigotti, Francesca, Vaquero-Siguero, Nuria, Fischer, Andreas, Rodriguez-Vita, Juan. 2023. HAPLN1 potentiates peritoneal metastasis in pancreatic cancer. In Nature communications, 14, 2353. doi:10.1038/s41467-023-38064-w. https://pubmed.ncbi.nlm.nih.gov/37095087/
2. Zhang, Tiancheng, Li, Xiang, He, Yani, Zhai, Jing, Shen, Lizong. 2021. Cancer-associated fibroblasts-derived HAPLN1 promotes tumour invasion through extracellular matrix remodeling in gastric cancer. In Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association, 25, 346-359. doi:10.1007/s10120-021-01259-5. https://pubmed.ncbi.nlm.nih.gov/34724589/
3. Chen, Yong, Wang, Baojiang, Chen, Yanjuan, Nandakumar, Kutty Selva, Liu, Dongzhou. 2022. HAPLN1 Affects Cell Viability and Promotes the Pro-Inflammatory Phenotype of Fibroblast-Like Synoviocytes. In Frontiers in immunology, 13, 888612. doi:10.3389/fimmu.2022.888612. https://pubmed.ncbi.nlm.nih.gov/35720292/
4. Chang, Hae Yeun, Huynh, Mailee, Roopra, Avtar, Callander, Natalie S, Miyamoto, Shigeki. . HAPLN1 matrikine: a bone marrow homing factor linked to poor outcomes in patients with MM. In Blood advances, 7, 6859-6872. doi:10.1182/bloodadvances.2023010139. https://pubmed.ncbi.nlm.nih.gov/37647592/
5. Kang, Jeong Suk, Kawakami, Yasuhiko, Bekku, Yoko, Izpisúa Belmonte, Juan Carlos, Oohashi, Toshitaka. . Molecular cloning and developmental expression of a hyaluronan and proteoglycan link protein gene, crtl1/hapln1, in zebrafish. In Zoological science, 25, 912-8. doi:10.2108/zsj.25.912. https://pubmed.ncbi.nlm.nih.gov/19267601/
6. Huynh, Mailee, Chang, Hae Yeun, Lisiero, Dominique N, Callander, Natalie S, Miyamoto, Shigeki. 2022. HAPLN1 confers multiple myeloma cell resistance to several classes of therapeutic drugs. In PloS one, 17, e0274704. doi:10.1371/journal.pone.0274704. https://pubmed.ncbi.nlm.nih.gov/36480501/
7. Yan, Tao, Song, Shushuai, Sun, Wendong, Ge, Yiping. 2024. HAPLN1 knockdown inhibits heart failure development via activating the PKA signaling pathway. In BMC cardiovascular disorders, 24, 197. doi:10.1186/s12872-024-03861-8. https://pubmed.ncbi.nlm.nih.gov/38580957/
8. Zhou, Guohui, Yan, Xinmin, Chen, Zhenfei, Zeng, Xing, Wu, Fangqian. 2023. ASPN Synergizes with HAPLN1 to Inhibit the Osteogenic Differentiation of Bone Marrow Mesenchymal Stromal Cells and Extracellular Matrix Mineralization of Osteoblasts. In Orthopaedic surgery, 15, 2423-2434. doi:10.1111/os.13803. https://pubmed.ncbi.nlm.nih.gov/37427673/