Dgkz-flox 基因敲除小鼠

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产品名称

Dgkz-flox 基因敲除小鼠

产品编号

S-CKO-18206

品系全称

C57BL/6JCya-Dgkzem1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-104418-Dgkz-B6J-VB

品系状态

使用本品系发表的文献需注明: Dgkz-flox 基因敲除小鼠 mice (Strain S-CKO-18206) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
diacylglycerol kinase zeta
基因别称
E130307B02Rik,F730209L11Rik,mDGK[z]
染色体号
Chr 2 (Mouse)
转录本 ID
NCBI: NM_138306 | Ensembl: ENSMUST00000028667
修饰方式
条件性基因敲除
靶向范围
Exon 2~4
敲除长度
~2.6 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1278339The T cell response is enhanced in homozygous mutant mice, which showed a robust response to viral infection.
DGKZ,也称为二酰甘油激酶ζ,是一种在哺乳动物细胞中发现的二酰甘油激酶。二酰甘油激酶是一类催化二酰甘油(DAG)转化为磷脂酸(PA)的酶,这个过程在细胞的信号转导中发挥着重要作用。DGKZ通过调节DAG的水平来影响下游的蛋白激酶C(PKC)的活性,进而影响细胞的生长、分化、凋亡和信号转导等过程。此外,DGKZ还参与脂筏的形成和功能,脂筏是细胞膜上富含胆固醇和鞘脂的区域,与细胞的信号转导、物质转运和细胞识别等过程密切相关。

DGKZ在多种疾病的发生和发展中发挥着重要作用。例如,DGKZ的表达水平与精神分裂症的发生密切相关[1]。研究发现,精神分裂症患者中DGKZ的表达水平显著高于健康对照组,并且DGKZ的基因型与精神分裂症的发生风险相关。此外,DGKZ还与T细胞的活化和功能密切相关[2]。通过CRISPR-Cas9技术,研究人员发现DGKZ的基因突变可以影响T细胞的活化和细胞因子的产生,进而影响免疫系统的功能。此外,DGKZ还与乳腺癌的转移密切相关[3]。研究发现,DGKZ的高表达可以促进乳腺癌细胞的转移,并且DGKZ通过调节TGFβ信号通路来影响乳腺癌的进展。此外,DGKZ还与冠状动脉疾病、肾上腺皮质癌、自闭症、骨肉瘤、肝细胞癌和克罗恩病等疾病的发生和发展密切相关[4][5][6][7][8][9][10]。

综上所述,DGKZ是一种重要的酶,在细胞的信号转导、脂筏的形成和功能以及多种疾病的发生和发展中发挥着重要作用。DGKZ的研究有助于深入理解细胞的信号转导和脂筏的生物学功能,为多种疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Alinaghi, Somayeh, Alehabib, Elham, Johari, Amir Hossein, Darvish, Hossein, Ghaedi, Hamid. 2019. Expression analysis and genotyping of DGKZ: a GWAS-derived risk gene for schizophrenia. In Molecular biology reports, 46, 4105-4111. doi:10.1007/s11033-019-04860-1. https://pubmed.ncbi.nlm.nih.gov/31087244/
2. Schmidt, Ralf, Ward, Carl C, Dajani, Rama, Dodgson, Stacie E, Marson, Alexander. 2023. Base-editing mutagenesis maps alleles to tune human T cell functions. In Nature, 625, 805-812. doi:10.1038/s41586-023-06835-6. https://pubmed.ncbi.nlm.nih.gov/38093011/
3. Zhao, Yuanyuan, Sun, Hefen, Li, Xuan, Hou, Yifeng, Jin, Wei. 2022. DGKZ promotes TGFβ signaling pathway and metastasis in triple-negative breast cancer by suppressing lipid raft-dependent endocytosis of TGFβR2. In Cell death & disease, 13, 105. doi:10.1038/s41419-022-04537-x. https://pubmed.ncbi.nlm.nih.gov/35115500/
4. Wang, Lang, Hu, Jun, Zhou, Jiali, Yao, Tan, Zhang, Liang. 2020. Weighed Gene Coexpression Network Analysis Screens the Potential Long Noncoding RNAs and Genes Associated with Progression of Coronary Artery Disease. In Computational and mathematical methods in medicine, 2020, 8183420. doi:10.1155/2020/8183420. https://pubmed.ncbi.nlm.nih.gov/32695216/
5. Rahane, Chinmay Satish, Kutzner, Arne, Heese, Klaus. 2018. Establishing a human adrenocortical carcinoma (ACC)-specific gene mutation signature. In Cancer genetics, 230, 1-12. doi:10.1016/j.cancergen.2018.10.005. https://pubmed.ncbi.nlm.nih.gov/30477734/
6. Chang, Suhua, Liu, Jia Jia, Zhao, Yilu, Yang, Li, Lu, Lin. 2024. Whole-genome sequencing identifies novel genes for autism in Chinese trios. In Science China. Life sciences, 67, 2368-2381. doi:10.1007/s11427-023-2564-8. https://pubmed.ncbi.nlm.nih.gov/39126614/
7. Yu, Wenxi, Tang, Lina, Lin, Feng, Yao, Yang, Shen, Zan. 2019. DGKZ Acts as a Potential Oncogene in Osteosarcoma Proliferation Through Its Possible Interaction With ERK1/2 and MYC Pathway. In Frontiers in oncology, 8, 655. doi:10.3389/fonc.2018.00655. https://pubmed.ncbi.nlm.nih.gov/30662872/
8. Peng, Xiaozhen, Lu, Xingjun, Yang, Daqing, Peng, Hong, Zhang, Yiya. . A novel CD8+ T cell-related gene signature as a prognostic biomarker in hepatocellular carcinoma. In Medicine, 103, e37496. doi:10.1097/MD.0000000000037496. https://pubmed.ncbi.nlm.nih.gov/38489709/
9. Harris, R Alan, Bush, Allyson H, Eagar, Todd N, Kugathasan, Subra, Kellermayer, Richard. 2023. Exome Sequencing Implicates DGKZ , ESRRA , and GXYLT1 for Modulating Granuloma Formation in Crohn Disease. In Journal of pediatric gastroenterology and nutrition, 77, 354-357. doi:10.1097/MPG.0000000000003873. https://pubmed.ncbi.nlm.nih.gov/37347142/
10. Öztan, Gözde, Bozbuğa, Nilgün, İşsever, Halim, Ertan, Melike, Alpagut, İbrahim Ufuk. 2023. Comparative Analysis of Transcriptome Profiles in Patients with Thromboangiitis Obliterans. In Genes, 15, . doi:10.3390/genes15010019. https://pubmed.ncbi.nlm.nih.gov/38275601/