Racgap1-flox 基因敲除小鼠

下单100%中奖,最高可得千元京东卡
复苏/繁育服务
产品名称

Racgap1-flox 基因敲除小鼠

产品编号

S-CKO-18185

品系全称

C57BL/6JCya-Racgap1em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-26934-Racgap1-B6J-VB

品系状态

使用本品系发表的文献需注明: Racgap1-flox 基因敲除小鼠 mice (Strain S-CKO-18185) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
Rac GTPase-activating protein 1
基因别称
Band25,GTPase,MgcRacGAP,gtl11,mKIAA1478
染色体号
Chr 15 (Mouse)
转录本 ID
NCBI: NM_012025 | Ensembl: ENSMUST00000171702
修饰方式
条件性基因敲除
靶向范围
Exon 4
敲除长度
~0.7 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1349423Embryos homozygous for a gene-trapped allele exhibit pre-implantation lethality associated with the formation of multinucleated blastomeres and failure to complete cytokinesis.
RacGAP1(Rac GTPase-activating protein 1)是一种重要的GTPase激活蛋白,参与调控Rac小G蛋白的活性。Rac小G蛋白是一类在细胞信号传导中发挥关键作用的蛋白质,参与细胞骨架重组、细胞迁移、细胞增殖和细胞凋亡等多种生物学过程。RacGAP1通过与Rac小G蛋白结合,催化其GTPase活性,使其从GTP结合状态转变为GDP结合状态,从而抑制Rac小G蛋白的活性,进而调控细胞的行为和功能。

在多种人类肿瘤中,RacGAP1的表达水平与肿瘤的发生、发展和预后密切相关。例如,在肺腺癌相关膜性肾病中,RacGAP1的表达水平升高,与患者的总生存期显著缩短相关[1]。此外,RacGAP1的表达水平还与多种人类癌症的预后相关,包括肺癌、结直肠癌和乳腺癌等[2]。在前列腺癌中,RacGAP1的表达水平升高,与内分泌治疗耐药相关[4]。在肝细胞癌中,RacGAP1与HIF-1α相互作用,共同促进肿瘤的进展[6]。

除了在肿瘤中的作用,RacGAP1还与多种其他疾病相关。例如,在系统性红斑狼疮(SLE)中,RacGAP1的表达水平升高,与患者的预后相关[3]。在先天性发育不良性贫血中,RacGAP1基因的突变与疾病的发生相关[2]。此外,RacGAP1还与先天性心脏病的发生相关[5]。

RacGAP1在多种疾病中发挥重要作用,包括肿瘤、SLE、先天性发育不良性贫血和先天性心脏病等。RacGAP1的表达水平和活性调控机制在疾病的发生、发展和预后中起着关键作用。RacGAP1的研究有助于深入理解GTPase激活蛋白的生物学功能和疾病发生机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Xu, Qianqian, Li, Jiayi, Zhuo, Li, Yang, Yue, Li, Wenge. 2024. RACGAP1 is a pivotal gene in lung adenocarcinoma-associated membranous nephropathy: Based on comprehensive bioinformatics analysis and machine learning. In International immunopharmacology, 139, 112783. doi:10.1016/j.intimp.2024.112783. https://pubmed.ncbi.nlm.nih.gov/39068752/
2. Hernández, Gonzalo, Romero-Cortadellas, Lídia, Ferrer-Cortès, Xènia, Segovia, Jose-Carlos, Sánchez, Mayka. 2023. Mutations in the RACGAP1 gene cause autosomal recessive congenital dyserythropoietic anemia type III. In Haematologica, 108, 581-587. doi:10.3324/haematol.2022.281277. https://pubmed.ncbi.nlm.nih.gov/36200420/
3. Li, Wenjie, Wang, Rong, Wang, Wei. 2023. Exploring the causality and pathogenesis of systemic lupus erythematosus in breast cancer based on Mendelian randomization and transcriptome data analyses. In Frontiers in immunology, 13, 1029884. doi:10.3389/fimmu.2022.1029884. https://pubmed.ncbi.nlm.nih.gov/36726984/
4. Wang, Jiajia, Liu, Hui, Yu, Zeyuan, Hu, Jing, Han, Bo. 2024. Reciprocal regulation between RACGAP1 and AR contributes to endocrine therapy resistance in prostate cancer. In Cell communication and signaling : CCS, 22, 339. doi:10.1186/s12964-024-01703-w. https://pubmed.ncbi.nlm.nih.gov/38898473/
5. Edwards, Jonathan J, Rouillard, Andrew D, Fernandez, Nicolas F, Ma'ayan, Avi, Gelb, Bruce D. 2020. Systems Analysis Implicates WAVE2 Complex in the Pathogenesis of Developmental Left-Sided Obstructive Heart Defects. In JACC. Basic to translational science, 5, 376-386. doi:10.1016/j.jacbts.2020.01.012. https://pubmed.ncbi.nlm.nih.gov/32368696/
6. Wu, Xianjian, Xu, Zuoming, Li, Wenchuan, Lu, Yuan, Pu, Jian. 2023. HIF‑1α and RACGAP1 promote the progression of hepatocellular carcinoma in a mutually regulatory way. In Molecular medicine reports, 28, . doi:10.3892/mmr.2023.13105. https://pubmed.ncbi.nlm.nih.gov/37772389/