Il34-flox 基因敲除小鼠

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产品名称

Il34-flox 基因敲除小鼠

产品编号

S-CKO-17650

品系全称

C57BL/6NCya-Il34em1flox/Cya

品系背景

C57BL/6NCya

品系编号

CKOCMP-76527-Il34-B6N-VC

品系状态

使用本品系发表的文献需注明: Il34-flox 基因敲除小鼠 mice (Strain S-CKO-17650) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
interleukin 34
基因别称
2010004A03Rik
染色体号
Chr 8 (Mouse)
转录本 ID
NCBI: NM_001135100 | Ensembl: ENSMUST00000076846
修饰方式
条件性基因敲除
靶向范围
Exon 3~5
敲除长度
~1.8 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1923777Mice homozygous for a knock-out allele exhibit reduced Langerhans cells and microglial cells in the skin and brain, respectively, with decreased susceptibility to type IV hypersensitivity reaction and fungal infection but increased susceptibility to viral infection.
Il34,也称为白细胞介素34,是一种重要的生长因子。它通过与CSF-1受体(CSF-1R)结合,在细胞增殖、分化和存活中发挥重要作用。Il34在多种组织中表达,包括巨噬细胞、皮肤朗格汉斯细胞、小肠潘氏细胞、脑部小胶质细胞等。Il34在多种生物学过程中发挥重要作用,包括免疫、炎症和神经退行性疾病。

Il34在肿瘤发生和发展中发挥重要作用。研究发现,Il34可以促进肿瘤相关巨噬细胞(TAMs)的M2型极化,抑制CD8+ T细胞介导的抗肿瘤免疫,从而促进肿瘤免疫逃逸[2]。此外,Il34还可以促进肿瘤相关成纤维细胞(CAFs)的增殖和存活,从而促进肿瘤的发生和发展[3,5]。Il34还可以促进血管平滑肌细胞(VSMCs)的增殖和分化,从而促进动脉粥样硬化斑块的形成[9]。

Il34在炎症和免疫中发挥重要作用。研究发现,Il34可以促进巨噬细胞的增殖、分化和存活,从而促进炎症反应[1]。此外,Il34还可以促进Th2型免疫应答,抑制Th1型免疫应答,从而促进过敏性疾病的发生和发展[4,8]。

Il34还可以促进小胶质细胞的自噬,从而保护中枢神经系统免受神经退行性疾病和神经炎症的影响[7]。此外,Il34还可以促进肾小管细胞的增殖和分化,从而保护肾脏免受高血压和肾脏疾病的影响[6]。

综上所述,Il34是一种重要的生长因子,参与调控细胞增殖、分化和存活,影响多种生物学过程。Il34在肿瘤发生和发展、炎症和免疫、神经退行性疾病和肾脏疾病中发挥重要作用。因此,Il34是疾病治疗和预防的重要靶点。

参考文献:
1. Stanley, E Richard, Chitu, Violeta. 2014. CSF-1 receptor signaling in myeloid cells. In Cold Spring Harbor perspectives in biology, 6, . doi:10.1101/cshperspect.a021857. https://pubmed.ncbi.nlm.nih.gov/24890514/
2. Nian, Zhigang, Dou, Yingchao, Shen, Yiqing, Tian, Zhigang, Wei, Haiming. 2024. Interleukin-34-orchestrated tumor-associated macrophage reprogramming is required for tumor immune escape driven by p53 inactivation. In Immunity, 57, 2344-2361.e7. doi:10.1016/j.immuni.2024.08.015. https://pubmed.ncbi.nlm.nih.gov/39321806/
3. Kobayashi, Hiroki, Gieniec, Krystyna A, Lannagan, Tamsin R M, Woods, Susan L, Worthley, Daniel L. 2021. The Origin and Contribution of Cancer-Associated Fibroblasts in Colorectal Carcinogenesis. In Gastroenterology, 162, 890-906. doi:10.1053/j.gastro.2021.11.037. https://pubmed.ncbi.nlm.nih.gov/34883119/
4. Marsella, Rosanna. 2021. Advances in our understanding of canine atopic dermatitis. In Veterinary dermatology, 32, 547-e151. doi:10.1111/vde.12965. https://pubmed.ncbi.nlm.nih.gov/33891338/
5. Wang, Ganggang, Zhou, Zhijie, Jin, Wenzhi, Zhang, Hao, Wang, Xiaoliang. 2023. Single-cell transcriptome sequencing reveals spatial distribution of IL34+ cancer-associated fibroblasts in hepatocellular carcinoma tumor microenvironment. In NPJ precision oncology, 7, 133. doi:10.1038/s41698-023-00483-9. https://pubmed.ncbi.nlm.nih.gov/38081923/
6. Abedini, Amin, Sánchez-Navaro, Andrea, Wu, Junnan, Kolkhof, Peter, Susztak, Katalin. 2024. Single-cell transcriptomics and chromatin accessibility profiling elucidate the kidney-protective mechanism of mineralocorticoid receptor antagonists. In The Journal of clinical investigation, 134, . doi:10.1172/JCI157165. https://pubmed.ncbi.nlm.nih.gov/37906287/
7. Berglund, Rasmus, Cheng, Yufei, Piket, Eliane, Guerreiro-Cacais, Andre Ortlieb, Jagodic, Maja. 2024. The aging mouse CNS is protected by an autophagy-dependent microglia population promoted by IL-34. In Nature communications, 15, 383. doi:10.1038/s41467-023-44556-6. https://pubmed.ncbi.nlm.nih.gov/38195627/
8. Fan, Baochao, Zhou, Jinzhu, Zhao, Yongxiang, Fan, Huiying, Li, Bin. . Identification of Cell Types and Transcriptome Landscapes of Porcine Epidemic Diarrhea Virus-Infected Porcine Small Intestine Using Single-Cell RNA Sequencing. In Journal of immunology (Baltimore, Md. : 1950), 210, 271-282. doi:10.4049/jimmunol.2101216. https://pubmed.ncbi.nlm.nih.gov/36548460/
9. Chou, Elizabeth L, Chaffin, Mark, Simonson, Bridget, Ellinor, Patrick T, Lindsay, Mark E. 2022. Aortic Cellular Diversity and Quantitative Genome-Wide Association Study Trait Prioritization Through Single-Nuclear RNA Sequencing of the Aneurysmal Human Aorta. In Arteriosclerosis, thrombosis, and vascular biology, 42, 1355-1374. doi:10.1161/ATVBAHA.122.317953. https://pubmed.ncbi.nlm.nih.gov/36172868/