Klf7-flox 基因敲除小鼠

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产品名称

Klf7-flox 基因敲除小鼠

产品编号

S-CKO-17134

品系全称

C57BL/6JCya-Klf7em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-93691-Klf7-B6J-VA

品系状态

使用本品系发表的文献需注明: Klf7-flox 基因敲除小鼠 mice (Strain S-CKO-17134) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
Kruppel-like transcription factor 7 (ubiquitous)
基因别称
9830124P08Rik
染色体号
Chr 1 (Mouse)
转录本 ID
NCBI: NM_033563 | Ensembl: ENSMUST00000114086
修饰方式
条件性基因敲除
靶向范围
Exon 2
敲除长度
~1.1 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1935151Homozygous null mice die within 3 days of life, showing lack of gastic milk, hypopnea, cyanosis, olfactory bulb hypoplasia, no response to tail clamping, impaired axon projection in the olfactory and visual systems, cerebral cortex and hippocampus, and reduced dendritic branching in the hippocampus.

发表文献

The FASEB Journal
2023-06-20
KLF7 promotes adipocyte inflammation and glucose metabolism disorder by activating the PKCζ/NF-κB pathway
Heliyon
2023-04
Stress-inducible IL-6 is regulated by KLF7 in brown adipocytes
Heliyon
2023-03-27
Stress-inducible IL-6 is regulated by KLF7 in brown adipocytes.
1
Klf7,即Krüppel-like factor 7,是一种锌指结构的转录因子,属于Kruppel-like因子(KLF)家族。KLF家族成员在多种生物学过程中发挥着关键作用,包括细胞分化、发育、代谢和疾病发生等。KLF7在多种组织和细胞类型中表达,包括心脏、血管、肝脏、脂肪组织和神经系统等。

KLF7在多种癌症中表达上调,与癌症的进展和不良预后相关。例如,KLF7在肝细胞癌(HCC)中表达上调,并通过上调Toll样受体4(TLR4)和蛋白酪氨酸激酶2(PTK2)的表达促进HCC的进展和转移[1]。此外,KLF7在头颈鳞状细胞癌(HNSCC)中也表达上调,并通过上调胰岛素样生长因子2 mRNA结合蛋白2(IGF2BP2)的表达促进HNSCC的进展[3]。在结直肠癌(CRC)中,KLF7通过抑制miR-139-5p的表达上调肿瘤蛋白D52(TPD52)的表达,从而增强CRC细胞的侵袭和迁移能力[4]。

KLF7在心血管系统中也发挥重要作用。研究发现,KLF7在冠状动脉疾病(CAD)中表达下调,且KLF7基因的变异与CAD的风险相关[2]。此外,KLF7在心脏和血管形成以及心血管发育中发挥重要作用。

KLF7在神经系统中也发挥着重要作用。研究发现,KLF7在先天性耳聋的澳大利亚短尾牛头犬(ASCD)中表达下调,且KLF7基因的变异与ASCD的先天性耳聋相关[5]。此外,KLF7在促进周围神经再生中发挥着重要作用[8]。

KLF7还参与调节脂肪细胞的增殖和分化。研究发现,KLF7通过激活Akt信号通路并顺式调节细胞周期蛋白依赖性激酶抑制剂3(CDKN3)的基因表达,促进前脂肪细胞的增殖[6]。此外,KLF7还可以与PU.1相互作用,抑制前脂肪细胞的分化并促进其增殖[7]。

综上所述,KLF7是一种重要的转录因子,在多种生物学过程中发挥着关键作用。KLF7在癌症、心血管系统、神经系统和脂肪细胞中表达上调,与多种疾病的发生和发展相关。KLF7的研究有助于深入理解KLF家族成员的生物学功能和疾病发生机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Feng, Weibo, Chen, Jie, Huang, Wenjie, Wu, Kaichun, Xia, Limin. 2023. HMGB1-mediated elevation of KLF7 facilitates hepatocellular carcinoma progression and metastasis through upregulating TLR4 and PTK2. In Theranostics, 13, 4042-4058. doi:10.7150/thno.84388. https://pubmed.ncbi.nlm.nih.gov/37554278/
2. Akbari Kordkheyli, Vahid, Poursheikhani, Arash, Sharobandi, Seyed Hasan, Hosseini, Sayed Mostafa. 2023. Analysis of KLF7 and KLF5 transcription factors gene variants in coronary artery disease. In Revista portuguesa de cardiologia : orgao oficial da Sociedade Portuguesa de Cardiologia = Portuguese journal of cardiology : an official journal of the Portuguese Society of Cardiology, 42, 835-843. doi:10.1016/j.repc.2023.03.017. https://pubmed.ncbi.nlm.nih.gov/37268267/
3. Cai, Hongshi, Liang, Jianfeng, Jiang, Yaoqi, Wang, Cheng, Hou, Jinsong. 2024. KLF7 regulates super-enhancer-driven IGF2BP2 overexpression to promote the progression of head and neck squamous cell carcinoma. In Journal of experimental & clinical cancer research : CR, 43, 69. doi:10.1186/s13046-024-02996-y. https://pubmed.ncbi.nlm.nih.gov/38443991/
4. Zhang, Juan, Li, Zhihan, Han, Jiaxu, Meng, Qingyu, Niu, Wenbo. 2024. KLF7 enhances the invasion and migration of colorectal cancer cells via the miR-139-5p/TPD52 axis. In Cancer biology & therapy, 25, 2385172. doi:10.1080/15384047.2024.2385172. https://pubmed.ncbi.nlm.nih.gov/39097779/
5. Xu, Fangzheng, Shan, Shuwen, Sommerlad, Susan, Seddon, Jennifer M, Brenig, Bertram. 2021. A Missense Mutation in the KLF7 Gene Is a Potential Candidate Variant for Congenital Deafness in Australian Stumpy Tail Cattle Dogs. In Genes, 12, . doi:10.3390/genes12040467. https://pubmed.ncbi.nlm.nih.gov/33805165/
6. Jia, Ziqiu, Jin, Zhao, Shao, Shuli, Zhang, Weiwei, Sun, Yingning. . KLF7 promotes preadipocyte proliferation via activation of the Akt signaling pathway by Cis-regulating CDKN3. In Acta biochimica et biophysica Sinica, 54, 1486-1496. doi:10.3724/abbs.2022144. https://pubmed.ncbi.nlm.nih.gov/36269137/
7. Tan, Ming, Xu, Hu, Li, Jinwei, Wang, Weiyu, Sun, Yingning. . PU.1 interacts with KLF7 to suppress differentiation and promote proliferation in chicken preadipocytes. In Acta biochimica et biophysica Sinica, 55, 143-153. doi:10.3724/abbs.2022202. https://pubmed.ncbi.nlm.nih.gov/36647727/
8. Wang, Ying, Li, Wen-Yuan, Jia, Hua, Guo, Su-Fen, Jin, Zai-Shun. 2016. KLF7-transfected Schwann cell graft transplantation promotes sciatic nerve regeneration. In Neuroscience, 340, 319-332. doi:10.1016/j.neuroscience.2016.10.069. https://pubmed.ncbi.nlm.nih.gov/27826105/

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