Hnrnpll-flox 基因敲除小鼠

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产品名称

Hnrnpll-flox 基因敲除小鼠

产品编号

S-CKO-15545

品系全称

C57BL/6JCya-Hnrnpllem1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-72692-Hnrnpll-B6J-VA

品系状态

使用本品系发表的文献需注明: Hnrnpll-flox 基因敲除小鼠 mice (Strain S-CKO-15545) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
heterogeneous nuclear ribonucleoprotein L-like
基因别称
2510028H02Rik,2810036L13Rik,Hnrpll
染色体号
Chr 17 (Mouse)
转录本 ID
NCBI: NM_144802 | Ensembl: ENSMUST00000184635
修饰方式
条件性基因敲除
靶向范围
Exon 3
敲除长度
~1.0 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1919942Mice homozygous for a point mutation in a RNA recognition motif of the gene product have defects in the generation of alternative transcripts normally found in memory T cells. Total CD4+ T cell counts are lower, with a reduction of nave CD44lo T cells occurring as mice age.
Hnrnpll(heterogeneous nuclear ribonucleoprotein L-like)是一种RNA结合蛋白,主要在细胞核中发挥作用。作为hnRNP家族的一员,Hnrnpll参与调控RNA的代谢过程,包括mRNA剪接、RNA稳定性和翻译调控等。近年来,研究发现Hnrnpll在淋巴细胞稳态和分化过程中发挥着重要作用。

Hnrnpll在淋巴细胞分化中的作用主要体现在对关键基因的剪接调控上。研究表明,Hnrnpll可以调控CD45RA到CD45RO的转换,这是记忆T细胞发育过程中的一个重要事件[1]。此外,Hnrnpll还参与了B细胞到浆细胞分化过程中的基因表达调控,通过调控Bcl6表达和Ig产生,影响浆细胞的成熟和功能[4]。Hnrnpll的缺失会导致淋巴细胞发育异常,如T细胞和B细胞数量减少,以及免疫功能受损。

除了在淋巴细胞分化中的作用,Hnrnpll还在其他生物学过程中发挥重要作用。研究表明,Hnrnpll在胚胎干细胞的多能性维持和分化过程中发挥着关键作用。Hnrnpll通过调控Tbx3和Bptf等转录因子的剪接,影响胚胎干细胞的多能性维持和分化[2]。此外,Hnrnpll还被发现是一种新的结直肠癌转移抑制因子,通过调控CD44的剪接影响结直肠癌细胞的侵袭和转移能力[3][7]。Hnrnpll的表达水平与结直肠癌的预后相关,其高表达与不良预后相关。

Hnrnpll的功能还与细胞周期和增殖相关。研究表明,Hnrnpll可以稳定DNA复制相关蛋白的mRNA,促进细胞周期进程和细胞增殖[5]。此外,Hnrnpll还可以通过调控CD44剪接影响卵巢癌的发生和发展[6]。

综上所述,Hnrnpll是一种重要的RNA结合蛋白,参与调控RNA的代谢过程,并在淋巴细胞分化、胚胎干细胞多能性维持和分化、结直肠癌转移抑制、细胞周期和增殖等多个生物学过程中发挥重要作用。Hnrnpll的研究有助于深入理解RNA结合蛋白的生物学功能和疾病发生机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Chang, Xing. 2016. RNA-binding protein hnRNPLL as a critical regulator of lymphocyte homeostasis and differentiation. In Wiley interdisciplinary reviews. RNA, 7, 295-302. doi:10.1002/wrna.1335. https://pubmed.ncbi.nlm.nih.gov/26821996/
2. Wang, Xue, Ping, Changyun, Tan, Puwen, Ma, Yanni, Huang, Yue. 2020. hnRNPLL controls pluripotency exit of embryonic stem cells by modulating alternative splicing of Tbx3 and Bptf. In The EMBO journal, 40, e104729. doi:10.15252/embj.2020104729. https://pubmed.ncbi.nlm.nih.gov/33349972/
3. Sakuma, Keiichiro, Sasaki, Eiichi, Kimura, Kenya, Yatabe, Yasushi, Aoki, Masahiro. 2017. HNRNPLL, a newly identified colorectal cancer metastasis suppressor, modulates alternative splicing of CD44 during epithelial-mesenchymal transition. In Gut, 67, 1103-1111. doi:10.1136/gutjnl-2016-312927. https://pubmed.ncbi.nlm.nih.gov/28360095/
4. Chang, Xing, Li, Bin, Rao, Anjana. 2015. RNA-binding protein hnRNPLL regulates mRNA splicing and stability during B-cell to plasma-cell differentiation. In Proceedings of the National Academy of Sciences of the United States of America, 112, E1888-97. doi:10.1073/pnas.1422490112. https://pubmed.ncbi.nlm.nih.gov/25825742/
5. Sakuma, Keiichiro, Sasaki, Eiichi, Kimura, Kenya, Yatabe, Yasushi, Aoki, Masahiro. 2018. HNRNPLL stabilizes mRNA for DNA replication proteins and promotes cell cycle progression in colorectal cancer cells. In Cancer science, 109, 2458-2468. doi:10.1111/cas.13660. https://pubmed.ncbi.nlm.nih.gov/29869816/
6. Yin, Huijing, Wang, Jingshu, Li, Hui, Ren, Chunxia, Yang, Gong. 2021. Extracellular matrix protein-1 secretory isoform promotes ovarian cancer through increasing alternative mRNA splicing and stemness. In Nature communications, 12, 4230. doi:10.1038/s41467-021-24315-1. https://pubmed.ncbi.nlm.nih.gov/34244494/
7. Sakuma, Keiichiro, Sasaki, Eiichi, Hosoda, Waki, Yatabe, Yasushi, Aoki, Masahiro. 2021. MYB mediates downregulation of the colorectal cancer metastasis suppressor heterogeneous nuclear ribonucleoprotein L-like during epithelial-mesenchymal transition. In Cancer science, 112, 3846-3855. doi:10.1111/cas.15069. https://pubmed.ncbi.nlm.nih.gov/34286904/