Ugt1a5-flox 基因敲除小鼠

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产品名称

Ugt1a5-flox 基因敲除小鼠

产品编号

S-CKO-11137

品系全称

C57BL/6JCya-Ugt1a5em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-394433-Ugt1a5-B6J-VA

品系状态

使用本品系发表的文献需注明: Ugt1a5-flox 基因敲除小鼠 mice (Strain S-CKO-11137) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
UDP glucuronosyltransferase 1 family, polypeptide A5
基因别称
-
染色体号
Chr 1 (Mouse)
转录本 ID
NCBI: NM_201643.2 | Ensembl: ENSMUST00000097659
修饰方式
条件性基因敲除
靶向范围
Exon 1
敲除长度
~1112 bp
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
UGT1A5,也称为UDP-葡萄糖醛酸基转移酶1A5,是UDP-葡萄糖醛酸基转移酶(UGTs)家族中的一员。UGTs是一类重要的Ⅱ相药物代谢酶,负责将亲脂性底物与葡萄糖醛酸结合,从而提高其水溶性并促进其排泄。UGTs在药物代谢、毒物解毒和内源性代谢物的处理中发挥着重要作用。

UGT1A5在多种组织中表达,包括肝脏、肠道、肾脏、肺、脑、睾丸和卵巢等。UGT1A5的表达具有组织特异性和性别特异性,例如在肝脏中,UGT1A5在女性中表达水平高于男性[10]。UGT1A5的表达受到多种因素的调控,包括激素信号、药物诱导和基因多态性等。

UGT1A5参与多种药物的代谢,包括胆红素、甾体激素、多环芳烃和致癌物质等。UGT1A5的活性受到多种因素的影响,包括底物结构、酶的表达水平和酶的遗传多态性等。UGT1A5的遗传多态性会影响其表达水平和活性,从而影响药物的代谢和疗效。

UGT1A5在多种疾病中发挥重要作用,包括肝细胞癌、结直肠癌和肺腺癌等。研究表明,UGT1A5的表达水平与肝细胞癌的预后和免疫治疗反应相关[2]。UGT1A5的表达水平在结直肠癌组织中降低,且与结直肠癌的发生发展相关[7]。UGT1A5的表达水平在肺腺癌组织中升高,且与肺腺癌的预后相关[6]。

综上所述,UGT1A5是一种重要的Ⅱ相药物代谢酶,参与多种药物的代谢和解毒,影响药物的疗效和安全性。UGT1A5的表达和活性受到多种因素的调控,包括激素信号、药物诱导和基因多态性等。UGT1A5在多种疾病中发挥重要作用,包括肝细胞癌、结直肠癌和肺腺癌等。深入研究UGT1A5的表达调控机制和功能,有助于提高药物治疗的个体化和精准性,为疾病的预防和治疗提供新的思路和策略[1][2][3][4][5][6][7][8][9][10]。

参考文献:
1. Neumann, Elizabeth, Mehboob, Huma, Ramírez, Jacqueline, Zhang, Min, Liu, Wanqing. 2016. Age-Dependent Hepatic UDP-Glucuronosyltransferase Gene Expression and Activity in Children. In Frontiers in pharmacology, 7, 437. doi:. https://pubmed.ncbi.nlm.nih.gov/27899892/
2. Wang, Yuxin, Wan, Xueshuai, Du, Shunda. 2023. Integrated analysis revealing a novel stemness-metabolism-related gene signature for predicting prognosis and immunotherapy response in hepatocellular carcinoma. In Frontiers in immunology, 14, 1100100. doi:10.3389/fimmu.2023.1100100. https://pubmed.ncbi.nlm.nih.gov/37622118/
3. Heydel, Jean-Marie, Garnier, Philippe, Faure, Philippe, Artur, Yves. 2012. Ciprofibrate regulation of rat hepatic bilirubin glucuronidation and UDP-glucuronosyltransferases expression. In European journal of drug metabolism and pharmacokinetics, 37, 233-40. doi:10.1007/s13318-012-0091-z. https://pubmed.ncbi.nlm.nih.gov/22476862/
4. Finel, Moshe, Li, Xin, Gardner-Stephen, Dione, Mackenzie, Peter I, Radominska-Pandya, Anna. 2005. Human UDP-glucuronosyltransferase 1A5: identification, expression, and activity. In The Journal of pharmacology and experimental therapeutics, 315, 1143-9. doi:. https://pubmed.ncbi.nlm.nih.gov/16120810/
5. Moe, Maren, Lien, Sigbjørn, Bendixen, Christian, Meuwissen, Theo H E, Grindflek, Eli. 2008. Gene expression profiles in liver of pigs with extreme high and low levels of androstenone. In BMC veterinary research, 4, 29. doi:10.1186/1746-6148-4-29. https://pubmed.ncbi.nlm.nih.gov/18684314/
6. Bao, Lianmin, Zhang, Yong, Wang, Jian, Xu, Menglin, Wang, Xiangdong. 2016. Variations of chromosome 2 gene expressions among patients with lung cancer or non-cancer. In Cell biology and toxicology, 32, 419-35. doi:10.1007/s10565-016-9343-z. https://pubmed.ncbi.nlm.nih.gov/27301951/
7. Wang, Min, Sun, De-Feng, Wang, Shuai, Luo, Ji-Zhuang, Li, Yan-Qing. 2013. Polymorphic expression of UDP-glucuronosyltransferase UGTlA gene in human colorectal cancer. In PloS one, 8, e57045. doi:10.1371/journal.pone.0057045. https://pubmed.ncbi.nlm.nih.gov/23468910/
8. Yeager, Ronnie L, Reisman, Scott A, Aleksunes, Lauren M, Klaassen, Curtis D. 2009. Introducing the "TCDD-inducible AhR-Nrf2 gene battery". In Toxicological sciences : an official journal of the Society of Toxicology, 111, 238-46. doi:10.1093/toxsci/kfp115. https://pubmed.ncbi.nlm.nih.gov/19474220/
9. Yang, Jing, Cai, Lei, Huang, Haiyan, Liu, Bingya, Wu, Qiang. 2012. Genetic variations and haplotype diversity of the UGT1 gene cluster in the Chinese population. In PloS one, 7, e33988. doi:10.1371/journal.pone.0033988. https://pubmed.ncbi.nlm.nih.gov/22514612/
10. Buckley, David B, Klaassen, Curtis D. 2006. Tissue- and gender-specific mRNA expression of UDP-glucuronosyltransferases (UGTs) in mice. In Drug metabolism and disposition: the biological fate of chemicals, 35, 121-7. doi:. https://pubmed.ncbi.nlm.nih.gov/17050650/