Tmem120b-flox 基因敲除小鼠

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产品名称

Tmem120b-flox 基因敲除小鼠

产品编号

S-CKO-10668

品系全称

C57BL/6JCya-Tmem120bem1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-330189-Tmem120b-B6J-VA

品系状态

使用本品系发表的文献需注明: Tmem120b-flox 基因敲除小鼠 mice (Strain S-CKO-10668) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
transmembrane protein 120B
基因别称
-
染色体号
Chr 5 (Mouse)
转录本 ID
NCBI: NM_001039723 | Ensembl: ENSMUST00000067505
修饰方式
条件性基因敲除
靶向范围
Exon 2~3
敲除长度
~2.7 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
Tmem120b基因编码一种跨膜蛋白,属于跨膜蛋白家族(TMEM)。跨膜蛋白家族成员广泛存在于细胞膜中,负责多种生物学功能,如信号传导、细胞骨架组织、细胞识别等。Tmem120b基因在多种组织和细胞类型中表达,包括脂肪组织、骨骼肌、神经系统等。

研究表明,Tmem120b基因在脂肪细胞分化过程中发挥重要作用。在脂肪细胞分化过程中,Tmem120b基因的表达上调,并与脂肪细胞分化相关基因的表达变化密切相关。例如,Tmem120b基因的敲低可以影响脂肪细胞分化相关基因如Gata3、Fasn和Glut4的表达,进而影响脂肪细胞的分化和代谢[2]。此外,Tmem120b基因的表达还与肥胖和脂质代谢相关。在肥胖小鼠的骨骼肌中,Tmem120b基因的表达下调,并可能与脂肪积累和肌肉萎缩相关[3]。此外,Tmem120b基因的表达还与卵母细胞核成熟相关。在多囊卵巢综合征(PCOS)患者中,Tmem120b基因的表达上调,并与卵母细胞核成熟相关[4]。此外,Tmem120b基因的表达还与肥胖和脂质代谢相关。在肥胖大鼠的脂肪组织中,Tmem120b基因的表达上调,并与肥胖相关基因的表达变化密切相关[5]。

研究表明,Tmem120b基因在多种疾病中发挥重要作用,包括肥胖、脂质代谢紊乱、多囊卵巢综合征等。Tmem120b基因的表达变化可能与这些疾病的发病机制相关,并可能成为治疗这些疾病的潜在靶点。此外,Tmem120b基因的表达还与癌症患者的预后相关。在骨肉瘤患者中,Tmem120b基因的表达与患者的总生存期相关。研究表明,Tmem120b基因的表达下调与骨肉瘤患者的良好预后相关[1]。此外,Tmem120b基因的表达还与肺癌患者的预后相关。研究表明,Tmem120b基因的表达上调与肺癌患者的较差预后相关[6]。Tmem120b基因的表达还与牙齿发育相关。研究表明,Tmem120b基因的表达与牙齿发育过程中的细胞黏附和细胞增殖相关[7]。此外,Tmem120b基因的表达还与马的遗传育种相关。研究表明,Tmem120b基因的纯合性缺失与马的细胞分化相关[8]。此外,Tmem120b基因的微缺失与智力障碍、自闭症、癫痫和颅面畸形等相关[9]。

综上所述,Tmem120b基因是一种重要的跨膜蛋白,参与调控脂肪细胞分化、肥胖、脂质代谢、多囊卵巢综合征、癌症、牙齿发育和马的遗传育种等多种生物学过程。Tmem120b基因的表达变化可能与这些疾病的发病机制相关,并可能成为治疗这些疾病的潜在靶点。

参考文献:
1. Du, Yuehui, Zeng, Xiaohui, Yu, Weiwei, Xie, Wei. 2022. A transmembrane protein family gene signature for overall survival prediction in osteosarcoma. In Frontiers in genetics, 13, 937300. doi:10.3389/fgene.2022.937300. https://pubmed.ncbi.nlm.nih.gov/35991561/
2. Batrakou, Dzmitry G, de Las Heras, Jose I, Czapiewski, Rafal, Mouras, Rabah, Schirmer, Eric C. 2015. TMEM120A and B: Nuclear Envelope Transmembrane Proteins Important for Adipocyte Differentiation. In PloS one, 10, e0127712. doi:10.1371/journal.pone.0127712. https://pubmed.ncbi.nlm.nih.gov/26024229/
3. Liao, Yuxiao, Peng, Zhao, Zhou, Xiaolei, Nüssler, Andreas K, Yang, Wei. 2024. Competing endogenous RNA networks were associated with fat accumulation in skeletal muscle of aged male mice. In Mechanisms of ageing and development, 220, 111953. doi:10.1016/j.mad.2024.111953. https://pubmed.ncbi.nlm.nih.gov/38834155/
4. Huang, Xin, Pan, Jiaping, Wu, Bi, Teng, Xiaoming. 2018. Construction and analysis of a lncRNA (PWRN2)-mediated ceRNA network reveal its potential roles in oocyte nuclear maturation of patients with PCOS. In Reproductive biology and endocrinology : RB&E, 16, 73. doi:10.1186/s12958-018-0392-4. https://pubmed.ncbi.nlm.nih.gov/30075721/
5. Crouse, Wesley L, Das, Swapan K, Le, Thu, Valdar, William, Solberg Woods, Leah C. 2022. Transcriptome-wide analyses of adipose tissue in outbred rats reveal genetic regulatory mechanisms relevant for human obesity. In Physiological genomics, 54, 206-219. doi:10.1152/physiolgenomics.00172.2021. https://pubmed.ncbi.nlm.nih.gov/35467982/
6. Liu, Li, Liu, Jun, Deng, Xiaoliang, Xie, Chenli, Yang, Lei. 2022. A nomogram based on A-to-I RNA editing predicting overall survival of patients with lung squamous carcinoma. In BMC cancer, 22, 715. doi:10.1186/s12885-022-09773-0. https://pubmed.ncbi.nlm.nih.gov/35768804/
7. Tang, Jia, Huang, Xiaofeng. 2024. Transcriptome analysis of human dental pulp cells cultured on a novel cell-adhesive fragment by RNA sequencing. In Gene, 927, 148709. doi:10.1016/j.gene.2024.148709. https://pubmed.ncbi.nlm.nih.gov/38901533/
8. Santos, Wellington B, Pereira, Camila B, Maiorano, Amanda M, Oliveira, Henrique N, Curi, Rogério A. 2023. Genomic inbreeding estimation, runs of homozygosity, and heterozygosity-enriched regions uncover signals of selection in the Quarter Horse racing line. In Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie, 140, 583-595. doi:10.1111/jbg.12812. https://pubmed.ncbi.nlm.nih.gov/37282810/
9. Labonne, Jonathan D J, Lee, Kang-Han, Iwase, Shigeki, Kim, Cheol-Hee, Kim, Hyung-Goo. 2016. An atypical 12q24.31 microdeletion implicates six genes including a histone demethylase KDM2B and a histone methyltransferase SETD1B in syndromic intellectual disability. In Human genetics, 135, 757-71. doi:10.1007/s00439-016-1668-4. https://pubmed.ncbi.nlm.nih.gov/27106595/