Tafa4-flox 基因敲除小鼠

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产品名称

Tafa4-flox 基因敲除小鼠

产品编号

S-CKO-10483

品系全称

C57BL/6JCya-Tafa4em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-320701-Tafa4-B6J-VA

品系状态

使用本品系发表的文献需注明: Tafa4-flox 基因敲除小鼠 mice (Strain S-CKO-10483) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
TAFA chemokine like family member 4
基因别称
C130034I18Rik,Fam19a4,Fam19a4 Tafa-4,Tafa-4
染色体号
Chr 6 (Mouse)
转录本 ID
NCBI: NM_177233 | Ensembl: ENSMUST00000089295
修饰方式
条件性基因敲除
靶向范围
Exon 3
敲除长度
~0.9 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:2444563Mice homozygous for a null allele exhibit increased mechanical and chemical nociception following inflammation or injury due to alterations in lamina IIi neuron excitability.
Tafa4,也称为FAM19A4,是一种在哺乳动物中表达的基因,其编码的蛋白质在多种生物学过程中发挥作用,包括感觉神经元的功能、免疫调节和细胞增殖。Tafa4在感觉神经元中表达,特别是在背根神经节(DRG)的C型无髓鞘感觉神经元中,这些神经元负责传导疼痛和温度感觉。研究表明,Tafa4在DRG神经元中的表达具有物种特异性,这意味着不同物种的DRG神经元中Tafa4的表达模式和功能可能存在差异[1]。

Tafa4不仅在感觉神经元中表达,还在免疫系统中发挥作用。研究表明,Tafa4可以调节肥大细胞中高亲和力IgE受体(FcεRI)的表达,抑制肥大细胞的活化和脱颗粒。FcεRI是过敏反应中的关键分子,其表达异常与过敏性疾病的发生发展密切相关。因此,Tafa4可能通过调节FcεRI的表达,在过敏反应和免疫调节中发挥重要作用[2]。

此外,Tafa4还与细胞增殖和肿瘤发生相关。研究发现,Tafa4在多种肿瘤组织中表达上调,包括宫颈癌、胃癌等。Tafa4的表达水平与肿瘤的发生发展、预后和免疫微环境密切相关。研究表明,Tafa4的表达可以影响肿瘤细胞的增殖、侵袭和转移,以及免疫细胞的功能和浸润。因此,Tafa4可能成为肿瘤诊断和治疗的新靶点[3,4,5,6,7,8,9,10]。

综上所述,Tafa4是一种重要的基因,在感觉神经元的功能、免疫调节和肿瘤发生中发挥重要作用。Tafa4的表达具有物种特异性,并在多种疾病中发挥作用。进一步研究Tafa4的生物学功能和作用机制,有助于深入理解其在疾病发生发展中的作用,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Jung, Min, Dourado, Michelle, Maksymetz, James, Riol-Blanco, Lorena, Kaminker, Joshua S. 2023. Cross-species transcriptomic atlas of dorsal root ganglia reveals species-specific programs for sensory function. In Nature communications, 14, 366. doi:10.1038/s41467-023-36014-0. https://pubmed.ncbi.nlm.nih.gov/36690629/
2. Zhou, Caijie, Li, Meihua, Liu, Yu, Huang, Qinmiao, Yang, Pingchang. . Signals from the TAFA4-PTEN-PU.1 axis alleviate nasal allergy by modulating the expression of FcεRI in mast cells. In Clinical and experimental immunology, 211, 15-22. doi:10.1093/cei/uxac097. https://pubmed.ncbi.nlm.nih.gov/36368013/
3. Louvanto, Karolina, Verhoef, Lisanne, Pimenoff, Ville, Nedjai, Belinda, Heideman, Daniëlle A M. 2024. Low methylation marker levels among human papillomavirus-vaccinated women with cervical high-grade squamous intraepithelial lesions. In International journal of cancer, 155, 1549-1557. doi:10.1002/ijc.35044. https://pubmed.ncbi.nlm.nih.gov/38801336/
4. Molano, Monica, Machalek, Dorothy A, Phillips, Samuel, Vallely, Andrew J, Murray, Gerald L. 2024. DNA methylation at individual CpG-sites of EPB41L3, HTERT and FAM19A4 are useful for detection of cervical high-grade squamous intraepithelial lesions (HSIL) or worse: Analysis of individual CpG-sites outperforms averaging. In Tumour virus research, 18, 200288. doi:10.1016/j.tvr.2024.200288. https://pubmed.ncbi.nlm.nih.gov/38960143/
5. Salio, Chiara, Aimar, Patrizia, Malapert, Pascale, Moqrich, Aziz, Merighi, Adalberto. 2020. Neurochemical and Ultrastructural Characterization of Unmyelinated Non-peptidergic C-Nociceptors and C-Low Threshold Mechanoreceptors Projecting to Lamina II of the Mouse Spinal Cord. In Cellular and molecular neurobiology, 41, 247-262. doi:10.1007/s10571-020-00847-w. https://pubmed.ncbi.nlm.nih.gov/32306148/
6. Peronace, Cinzia, Cione, Erika, Abrego-Guandique, Diana Marisol, Cannataro, Roberto, Minchella, Pasquale. 2024. FAM19A4 and hsa-miR124-2 Double Methylation as Screening for ASC-H- and CIN1 HPV-Positive Women. In Pathogens (Basel, Switzerland), 13, . doi:10.3390/pathogens13040312. https://pubmed.ncbi.nlm.nih.gov/38668267/
7. Vink, Frederique J, Meijer, Chris J L M, Clifford, Gary M, Bleeker, Maaike C G, Heideman, Daniëlle A M. 2019. FAM19A4/miR124-2 methylation in invasive cervical cancer: A retrospective cross-sectional worldwide study. In International journal of cancer, 147, 1215-1221. doi:10.1002/ijc.32614. https://pubmed.ncbi.nlm.nih.gov/31390052/
8. Li, Y, He, X, Fan, L, Xu, Y, Xu, X. 2020. Identification of a novel immune prognostic model in gastric cancer. In Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 23, 846-855. doi:10.1007/s12094-020-02478-5. https://pubmed.ncbi.nlm.nih.gov/32857339/
9. Cheng, Xiaobo, Chai, Ranran, Zhang, Teng, Luo, Weifeng, Kang, Yu. 2024. A novel methylation-detection panel for HPV associated high-grade squamous intraepithelial lesion and cervical cancer screening. In Scientific reports, 14, 25556. doi:10.1038/s41598-024-75047-3. https://pubmed.ncbi.nlm.nih.gov/39462105/
10. Reynders, Ana, Moqrich, Aziz. 2015. Analysis of cutaneous MRGPRD free nerve endings and C-LTMRs transcriptomes by RNA-sequencing. In Genomics data, 5, 132-5. doi:10.1016/j.gdata.2015.05.022. https://pubmed.ncbi.nlm.nih.gov/26484241/