Tnfrsf12a-flox 基因敲除小鼠

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产品名称

Tnfrsf12a-flox 基因敲除小鼠

产品编号

S-CKO-09994

品系全称

C57BL/6JCya-Tnfrsf12aem1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-27279-Tnfrsf12a-B6J-VA

品系状态

使用本品系发表的文献需注明: Tnfrsf12a-flox 基因敲除小鼠 mice (Strain S-CKO-09994) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
tumor necrosis factor receptor superfamily, member 12a
基因别称
Fn14,HPIP,TWEAK-R,TweakR
染色体号
Chr 17 (Mouse)
转录本 ID
NCBI: NM_013749 | Ensembl: ENSMUST00000024698
修饰方式
条件性基因敲除
靶向范围
Exon 2~4
敲除长度
~1.0 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1351484Mice homozygous for a knock-out allele exhibit significantly reduced liver progenitor ("oval") cell proliferation in response to chemically-induced liver injury.
TNFRSF12A,也称为Fn14,是一种属于肿瘤坏死因子受体超家族的细胞表面受体。它是由Tnfrsf12a基因编码的,这个基因位于人染色体3p25.3区域。TNFRSF12A能够与TNF超家族的成员TWEAK(Tnfsf12)结合,从而触发多种生物学效应,包括细胞增殖、凋亡、炎症反应以及免疫调节等。

在肿瘤微环境中,TNFRSF12A的表达水平往往与肿瘤的进展和预后相关。例如,在胶质瘤中,TNFRSF12A的表达与不良预后相关。研究显示,TNFRSF12A可能通过诱导M2巨噬细胞的极化来促进胶质瘤的进展[1]。M2巨噬细胞是一种具有免疫抑制作用的巨噬细胞亚型,它们能够促进肿瘤的生长和转移。因此,TNFRSF12A可能成为治疗胶质瘤的新靶点。

除了在胶质瘤中的作用,TNFRSF12A还与其他多种癌症相关。例如,在结直肠癌中,TNFRSF12A的表达上调与肿瘤的生长和转移相关。研究显示,TNFRSF12A可能通过激活NF-κB信号通路和上调BIRC3的表达来促进结直肠癌的进展[5]。此外,TNFRSF12A的表达还与多种癌症的预后相关,如肝细胞癌和甲状腺癌[6,7]。

除了在癌症中的作用,TNFRSF12A还与多种炎症性疾病相关。例如,在银屑病中,TNFRSF12A的表达上调与皮肤炎症相关。研究显示,TNFRSF12A可能通过与TNF和IL-17协同作用来增强炎症反应[8]。此外,TNFRSF12A还与急性肾损伤相关。研究显示,TNFRSF12A的表达上调与急性肾损伤的炎症反应相关[9]。

综上所述,TNFRSF12A是一种重要的细胞表面受体,参与调控多种生物学效应,包括细胞增殖、凋亡、炎症反应以及免疫调节等。在癌症和炎症性疾病中,TNFRSF12A的表达水平往往与疾病的进展和预后相关。因此,TNFRSF12A可能成为治疗这些疾病的新靶点。

参考文献:
1. Zhang, Xiaobin, Sun, Xiaolin, Guo, Chen, Li, Jianan, Liang, Guobiao. 2023. Cancer-associated fibroblast-associated gene IGFBP2 promotes glioma progression through induction of M2 macrophage polarization. In American journal of physiology. Cell physiology, 326, C252-C268. doi:10.1152/ajpcell.00234.2023. https://pubmed.ncbi.nlm.nih.gov/37982173/
2. Ruiz, Bryan I, Lowman, Xazmin H, Yang, Ying, Hanse, Eric A, Kong, Mei. 2023. Alpha-Ketoglutarate Regulates Tnfrsf12a/Fn14 Expression via Histone Modification and Prevents Cancer-Induced Cachexia. In Genes, 14, . doi:10.3390/genes14091818. https://pubmed.ncbi.nlm.nih.gov/37761958/
3. Wang, Tiantian, Shi, Jinyuan, Li, Luchuan, Liu, Lian, Sheng, Lei. 2022. Single-Cell Transcriptome Analysis Reveals Inter-Tumor Heterogeneity in Bilateral Papillary Thyroid Carcinoma. In Frontiers in immunology, 13, 840811. doi:10.3389/fimmu.2022.840811. https://pubmed.ncbi.nlm.nih.gov/35515000/
4. Xiao, Man-Huan, Ma, Dong, Wu, Sihan, Feng, Xuyang, Luo, Zhenhua. 2024. Integrative single-cell and spatial transcriptomic analyses identify a pathogenic cholangiocyte niche and TNFRSF12A as therapeutic target for biliary atresia. In Hepatology (Baltimore, Md.), 81, 1146-1163. doi:10.1097/HEP.0000000000001064. https://pubmed.ncbi.nlm.nih.gov/39178365/
5. Wang, Chuyue, Zhao, Yingying, Chen, You, Sun, Yifeng, Yuan, Ping. 2024. The Oncogenic Role of TNFRSF12A in Colorectal Cancer and Pan-Cancer Bioinformatics Analysis. In Cancer research and treatment, 57, 212-228. doi:10.4143/crt.2024.408. https://pubmed.ncbi.nlm.nih.gov/39118523/
6. Wang, Tao, Ma, Sicong, Qi, Xingxing, Li, Ping, Zhai, Bo. 2017. Knockdown of the differentially expressed gene TNFRSF12A inhibits hepatocellular carcinoma cell proliferation and migration in vitro. In Molecular medicine reports, 15, 1172-1178. doi:10.3892/mmr.2017.6154. https://pubmed.ncbi.nlm.nih.gov/28138696/
7. Chen, Yiwei, Lin, Jiali, Shi, Detong, Wang, Xiaotao, Zhang, Changqing. 2025. Identification of WDR74 and TNFRSF12A as biomarkers for early osteoarthritis using machine learning and immunohistochemistry. In Frontiers in immunology, 16, 1517646. doi:10.3389/fimmu.2025.1517646. https://pubmed.ncbi.nlm.nih.gov/39935469/
8. Gupta, Rinkesh K, Gracias, Donald T, Figueroa, Daniela Salgado, Burkly, Linda, Croft, Michael. 2021. TWEAK functions with TNF and IL-17 on keratinocytes and is a potential target for psoriasis therapy. In Science immunology, 6, eabi8823. doi:10.1126/sciimmunol.abi8823. https://pubmed.ncbi.nlm.nih.gov/34797693/
9. Li, Xiaoyang, Zhou, Xiangyang, Ping, Xinbo, Li, Rongshang, Guo, Lili. 2024. Combined Plasma Olink Proteomics and Transcriptomics Identifies CXCL1 and TNFRSF12A as Potential Predictive and Diagnostic Inflammatory Markers for Acute Kidney Injury. In Inflammation, 47, 1547-1563. doi:10.1007/s10753-024-01993-9. https://pubmed.ncbi.nlm.nih.gov/38472598/