Rab6b-flox 基因敲除小鼠

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产品名称

Rab6b-flox 基因敲除小鼠

产品编号

S-CKO-09888

品系全称

C57BL/6JCya-Rab6bem1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-270192-Rab6b-B6J-VA

品系状态

使用本品系发表的文献需注明: Rab6b-flox 基因敲除小鼠 mice (Strain S-CKO-09888) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
RAB6B, member RAS oncogene family
基因别称
9630015C03,C330006L04Rik,D9Bwg0185e
染色体号
Chr 9 (Mouse)
转录本 ID
NCBI: NM_173781.4 | Ensembl: ENSMUST00000035155
修饰方式
条件性基因敲除
靶向范围
Exon 2
敲除长度
~1.2 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:107283Homozygous mutation of this gene results in growth retardation and multiple behavioral and immunological abnormalities.
Rab6b是Rab小GTP酶家族的一个成员,它在细胞内的膜运输途径中起着重要的调节作用。Rab6b蛋白与Rab6A蛋白有91%的氨基酸序列一致性,但它们在细胞类型特异性和定位上有所不同。Rab6b主要在脑组织和神经母细胞瘤细胞系中表达,并定位于高尔基体和ERGIC-53阳性囊泡中。与Rab6A相比,Rab6B表现出较低的GTP结合活性,并且在过表达研究中,Rab6B蛋白分布在高尔基体和ER膜上,而Rab6A则更局限于高尔基体。这些结果表明,Rab6B在高尔基体水平的逆向膜运输中具有细胞类型特异性的作用[5]。

Rab6b在多种疾病中发挥重要作用。例如,在动脉粥样硬化中,Rab6b的表达下调,可能参与维持血管稳态。在胃癌中,miR-4268通过靶向Rab6B抑制细胞增殖和细胞周期G1/S期转换,并诱导细胞凋亡。此外,Rab6b在癫痫和胶质瘤中也被发现是保守的基因之一,参与突触信号传导和钙离子途径。在结直肠癌中,Rab6B表达下调,与较差的预后相关。这些研究表明,Rab6b在细胞生物学和疾病发生中具有重要作用,可能成为潜在的生物标志物和治疗靶点[1][2][3][4][6][7][8][9]。

总之,Rab6b是一种重要的Rab小GTP酶,参与细胞内膜运输途径的调节。它在多种疾病中发挥重要作用,包括动脉粥样硬化、胃癌、癫痫、胶质瘤和结直肠癌。Rab6b可能成为潜在的生物标志物和治疗靶点,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Zhao, Kai, Bai, Xuexue, Wang, Xiao, Li, Wei, Wang, Shiyong. 2023. Insight on the hub gene associated signatures and potential therapeutic agents in epilepsy and glioma. In Brain research bulletin, 199, 110666. doi:10.1016/j.brainresbull.2023.110666. https://pubmed.ncbi.nlm.nih.gov/37192718/
2. Logan, Jeongok G, Yun, Sijung, Bao, Yongde, Farber, Emily, Farber, Charles R. 2020. RNA-sequencing analysis of differential gene expression associated with arterial stiffness. In Vascular, 28, 655-663. doi:10.1177/1708538120922650. https://pubmed.ncbi.nlm.nih.gov/32375599/
3. Willemsen, Sam, Yengej, Fjodor A Yousef, Puschhof, Jens, Beumer, Joep, Clevers, Hans. 2024. A comprehensive transcriptome characterization of individual nuclear receptor pathways in the human small intestine. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2411189121. doi:10.1073/pnas.2411189121. https://pubmed.ncbi.nlm.nih.gov/39475639/
4. Wang, Cancan, Zhang, Ran, Zhang, Huan, Zhou, Meiyu, Li, Xinxia. 2024. Lipid metabolism-related gene signature predicts prognosis and unveils novel anti-tumor drugs in specific type of diffuse large B cell lymphoma. In Molecular medicine (Cambridge, Mass.), 30, 210. doi:10.1186/s10020-024-00988-4. https://pubmed.ncbi.nlm.nih.gov/39538125/
5. Opdam, F J, Echard, A, Croes, H J, Goud, B, Fransen, J A. . The small GTPase Rab6B, a novel Rab6 subfamily member, is cell-type specifically expressed and localised to the Golgi apparatus. In Journal of cell science, 113 ( Pt 15), 2725-35. doi:. https://pubmed.ncbi.nlm.nih.gov/10893188/
6. Zhao, Lingyu, Xue, Meng, Zhang, Lu, Huang, Chen, Tong, Dongdong. 2019. MicroRNA-4268 inhibits cell proliferation via AKT/JNK signalling pathways by targeting Rab6B in human gastric cancer. In Cancer gene therapy, 27, 461-472. doi:10.1038/s41417-019-0118-6. https://pubmed.ncbi.nlm.nih.gov/31303644/
7. Kang, Jinlin, Li, Na, Wang, Fen, Peng, Jin, Zhou, Fuxiang. 2022. Exploration of Reduced Mitochondrial Content-Associated Gene Signature and Immunocyte Infiltration in Colon Adenocarcinoma by an Integrated Bioinformatic Analysis. In Frontiers in genetics, 13, 832331. doi:10.3389/fgene.2022.832331. https://pubmed.ncbi.nlm.nih.gov/35464857/
8. Ahbara, Abulgasim M, Khbou, Médiha Khamassi, Rhomdhane, Rihab, Rischkowsky, Barbara, Mwacharo, Joram M. 2022. Genome variation in tick infestation and cryptic divergence in Tunisian indigenous sheep. In BMC genomics, 23, 167. doi:10.1186/s12864-022-08321-1. https://pubmed.ncbi.nlm.nih.gov/35227193/
9. Jiang, Xuefei, Yang, Lanlan, Gao, Qianling, Ye, Shubiao, Yang, Zihuan. 2022. The Role of RAB GTPases and Its Potential in Predicting Immunotherapy Response and Prognosis in Colorectal Cancer. In Frontiers in genetics, 13, 828373. doi:10.3389/fgene.2022.828373. https://pubmed.ncbi.nlm.nih.gov/35154286/