Olfm2-flox 基因敲除小鼠

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产品名称

Olfm2-flox 基因敲除小鼠

产品编号

S-CKO-08628

品系全称

C57BL/6JCya-Olfm2em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-244723-Olfm2-B6J-VA

品系状态

使用本品系发表的文献需注明: Olfm2-flox 基因敲除小鼠 mice (Strain S-CKO-08628) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
olfactomedin 2
基因别称
A030009A06Rik
染色体号
Chr 9 (Mouse)
转录本 ID
NCBI: NM_173777 | Ensembl: ENSMUST00000034692
修饰方式
条件性基因敲除
靶向范围
Exon 6
敲除长度
~15.9 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:3045350Mice homozygous for a knock-out allele exhibit moderate locomotor and anxiety-related behavioral abnormalities, altered visual evoked potential, and reduced compactness of myelin sheaths in the optic nerve.
OLFM2,也称为olfactomedin 2,是一种分泌性糖蛋白,属于olfactomedin结构域家族。这个家族的蛋白质在细胞粘附、信号转导和细胞分化中发挥着重要作用。OLFM2基因位于人类19号染色体上,编码一个含有olfactomedin结构域的蛋白质,这个结构域在许多细胞过程中发挥着关键作用。

OLFM2在多种生物学过程中发挥着重要作用。首先,它参与了上皮-间质转化(EMT)的过程,这是一个关键的细胞过程,在肿瘤发生和转移中起着重要作用。OLFM2通过促进EMT,使得肿瘤细胞能够获得侵袭性和迁移性,从而促进肿瘤的转移和扩散[1]。其次,OLFM2还参与了平滑肌细胞的分化和血管重塑的过程。它通过调节转录因子SRF与CArG盒的结合,影响平滑肌细胞的表型和功能,从而影响血管的稳定性和重塑[2,8]。

此外,OLFM2还与一些神经系统的功能有关。研究表明,OLFM2在视网膜和嗅觉系统中发挥着重要作用。OLFM2与AMPA受体复合物相互作用,影响神经元的信号传递和功能[3,6]。此外,OLFM2的基因变异还与一些神经系统的疾病有关,如青光眼和眼部发育异常[4,5,9]。

最近的研究还发现,OLFM2在结直肠癌(CRC)的发生和进展中发挥着重要作用。OLFM2在CRC组织中高表达,并且其表达水平与CRC的侵袭性和转移性相关。研究发现,OLFM2通过促进EMT和调节TGF-β/Smad信号通路,促进CRC的侵袭和转移[1]。此外,OLFM2还与结直肠癌的预后相关,其高表达与不良预后相关[7]。

综上所述,OLFM2是一种重要的蛋白质,在多种生物学过程中发挥着重要作用。它参与了上皮-间质转化、平滑肌细胞的分化和血管重塑、神经系统的功能等多个过程。此外,OLFM2还与结直肠癌的发生和进展相关。因此,OLFM2可能成为结直肠癌诊断和治疗的重要靶点,为结直肠癌的治疗和预防提供新的思路和策略。

参考文献:
1. Tang, Yong, Liu, Yi, Wang, Xiaobo, Chen, Xinrui, Wang, Xianfei. 2024. OLFM2 promotes epithelial-mesenchymal transition, migration, and invasion in colorectal cancer through the TGF-β/Smad signaling pathway. In BMC cancer, 24, 204. doi:10.1186/s12885-024-11925-3. https://pubmed.ncbi.nlm.nih.gov/38350902/
2. Shi, Ning, Guo, Xia, Chen, Shi-You. 2014. Olfactomedin 2, a novel regulator for transforming growth factor-β-induced smooth muscle differentiation of human embryonic stem cell-derived mesenchymal cells. In Molecular biology of the cell, 25, 4106-14. doi:10.1091/mbc.E14-08-1255. https://pubmed.ncbi.nlm.nih.gov/25298399/
3. Sultana, Afia, Nakaya, Naoki, Dong, Lijin, Qian, Haohua, Tomarev, Stanislav I. 2014. Deletion of olfactomedin 2 induces changes in the AMPA receptor complex and impairs visual, olfactory, and motor functions in mice. In Experimental neurology, 261, 802-11. doi:10.1016/j.expneurol.2014.09.002. https://pubmed.ncbi.nlm.nih.gov/25218043/
4. Funayama, Tomoyo, Mashima, Yukihiko, Ohtake, Yuichiro, Hotta, Yoshihiro, Shimada, Naoki. . SNPs and interaction analyses of noelin 2, myocilin, and optineurin genes in Japanese patients with open-angle glaucoma. In Investigative ophthalmology & visual science, 47, 5368-75. doi:. https://pubmed.ncbi.nlm.nih.gov/17122126/
5. Holt, R, Ugur Iseri, S A, Wyatt, A W, Zhang, Y, Ragge, Nicola. 2016. Identification and functional characterisation of genetic variants in OLFM2 in children with developmental eye disorders. In Human genetics, 136, 119-127. doi:10.1007/s00439-016-1745-8. https://pubmed.ncbi.nlm.nih.gov/27844144/
6. Sultana, Afia, Nakaya, Naoki, Senatorov, Vladimir V, Tomarev, Stanislav I. 2011. Olfactomedin 2: expression in the eye and interaction with other olfactomedin domain-containing proteins. In Investigative ophthalmology & visual science, 52, 2584-92. doi:10.1167/iovs.10-6356. https://pubmed.ncbi.nlm.nih.gov/21228389/
7. Schulten, Hans-Juergen, Hussein, Deema, Al-Adwani, Fatima, Chaudhary, Adeel, Al-Qahtani, Mohammed. 2016. Microarray Expression Data Identify DCC as a Candidate Gene for Early Meningioma Progression. In PloS one, 11, e0153681. doi:10.1371/journal.pone.0153681. https://pubmed.ncbi.nlm.nih.gov/27096627/
8. Shi, Ning, Li, Chen-Xiao, Cui, Xiao-Bing, Tomarev, Stanislav I, Chen, Shi-You. 2017. Olfactomedin 2 Regulates Smooth Muscle Phenotypic Modulation and Vascular Remodeling Through Mediating Runt-Related Transcription Factor 2 Binding to Serum Response Factor. In Arteriosclerosis, thrombosis, and vascular biology, 37, 446-454. doi:10.1161/ATVBAHA.116.308606. https://pubmed.ncbi.nlm.nih.gov/28062493/
9. Zhang, Ruoyan, Ye, Junfeng, Huang, Heyu, Du, Xiaohong. 2019. Mining featured biomarkers associated with vascular invasion in HCC by bioinformatics analysis with TCGA RNA sequencing data. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 118, 109274. doi:10.1016/j.biopha.2019.109274. https://pubmed.ncbi.nlm.nih.gov/31545220/