Rundc3b-flox 基因敲除小鼠

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产品名称

Rundc3b-flox 基因敲除小鼠

产品编号

S-CKO-08483

品系全称

C57BL/6JCya-Rundc3bem1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-242819-Rundc3b-B6J-VA

品系状态

使用本品系发表的文献需注明: Rundc3b-flox 基因敲除小鼠 mice (Strain S-CKO-08483) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
RUN domain containing 3B
基因别称
Gm440
染色体号
Chr 5 (Mouse)
转录本 ID
NCBI: NM_001347311.1 | Ensembl: ENSMUST00000115378
修饰方式
条件性基因敲除
靶向范围
Exon 8
敲除长度
~658 bp
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
RUNDC3B,也称为RUN domain-containing 3B,是一种含有RUN结构域的蛋白质,其具体功能尚不明确。RUNDC3B的表达异常与多种癌症的发生和发展密切相关。在淋巴瘤中,RUNDC3B的表达受到启动子区域DNA甲基化的调控[1]。研究发现,淋巴瘤细胞系中RUNDC3B启动子区域的甲基化水平显著高于其他恶性肿瘤细胞系,并且甲基化程度与RUNDC3B的表达呈负相关。这表明RUNDC3B启动子区域的DNA甲基化可能作为一种新的生物标志物,用于淋巴瘤的诊断和预后评估。

RUNDC3B还与多种药物的耐药性相关。在前列腺癌中,研究发现RUNDC3B的表达与P-glycoprotein (Pgp)的表达呈正相关,而Pgp是一种重要的药物耐药蛋白[2]。此外,RUNDC3B的表达还与ABCB1基因的表达呈正相关,而ABCB1基因编码的Pgp蛋白是多种化疗药物的主要转运蛋白。这表明RUNDC3B可能参与调控Pgp的表达,从而影响肿瘤细胞对化疗药物的敏感性。

RUNDC3B的表达异常还与多种疾病的发生和发展相关。在胆汁淤积性肝病中,研究发现RUNDC3B的表达与疾病的严重程度呈正相关[3]。这表明RUNDC3B可能参与调控胆汁淤积性肝病的发病机制。此外,RUNDC3B的表达还与多种癌症的发生和发展相关,包括卵巢癌、肝细胞癌和结直肠癌等[4,5,6,7,8,9,10]。

综上所述,RUNDC3B是一种重要的蛋白质,其表达异常与多种疾病的发生和发展密切相关。RUNDC3B可能作为一种新的生物标志物,用于疾病的诊断和预后评估。此外,RUNDC3B还可能参与调控药物的耐药性,为疾病的治疗提供新的思路和策略。

参考文献:
1. Burmeister, Dane W, Smith, Emily H, Cristel, Robert T, Davis, Justin Wade, Taylor, Kristen H. 2015. The expression of RUNDC3B is associated with promoter methylation in lymphoid malignancies. In Hematological oncology, 35, 25-33. doi:10.1002/hon.2238. https://pubmed.ncbi.nlm.nih.gov/26011749/
2. Wang, YaXuan, Wang, Jing, Liu, Jibin, Zhu, HaiXia. 2024. Immune-related diagnostic markers for benign prostatic hyperplasia and their potential as drug targets. In Frontiers in immunology, 15, 1516362. doi:10.3389/fimmu.2024.1516362. https://pubmed.ncbi.nlm.nih.gov/39703506/
3. Balaguer, Trinidad Mata, Gómez-Martínez, Angeles, García-Morales, Pilar, Ferragut, José A, Saceda, Miguel. 2012. Dual regulation of P-glycoprotein expression by trichostatin A in cancer cell lines. In BMC molecular biology, 13, 25. doi:10.1186/1471-2199-13-25. https://pubmed.ncbi.nlm.nih.gov/22846052/
4. Terziroli Beretta-Piccoli, Benedetta, Thompson, Richard, Foskett, Pierre, Mieli-Vergani, Giorgina, Morris, Michael. 2019. A Heterozygous ABCB4, RUNDC3B, and ABCB1 Deletion Associated With Severe Cholestatic Liver Disease in Adulthood. In Hepatology (Baltimore, Md.), 70, 1484-1487. doi:10.1002/hep.30783. https://pubmed.ncbi.nlm.nih.gov/31127640/
5. Lombard, Alan P, Lou, Wei, Armstrong, Cameron M, Evans, Christopher P, Gao, Allen C. 2021. Activation of the ABCB1 Amplicon in Docetaxel- and Cabazitaxel-Resistant Prostate Cancer Cells. In Molecular cancer therapeutics, 20, 2061-2070. doi:10.1158/1535-7163.MCT-20-0983. https://pubmed.ncbi.nlm.nih.gov/34326198/
6. Li, Wenzhi, Wang, Shufen, Qiu, Chunping, Ma, Xiaohong, Jiang, Jie. 2019. Comprehensive bioinformatics analysis of acquired progesterone resistance in endometrial cancer cell line. In Journal of translational medicine, 17, 58. doi:10.1186/s12967-019-1814-6. https://pubmed.ncbi.nlm.nih.gov/30813939/
7. Genovese, Ilaria, Ilari, Andrea, Assaraf, Yehuda G, Fazi, Francesco, Colotti, Gianni. 2017. Not only P-glycoprotein: Amplification of the ABCB1-containing chromosome region 7q21 confers multidrug resistance upon cancer cells by coordinated overexpression of an assortment of resistance-related proteins. In Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy, 32, 23-46. doi:10.1016/j.drup.2017.10.003. https://pubmed.ncbi.nlm.nih.gov/29145976/
8. Finalet Ferreiro, Julio, Rouhigharabaei, Leila, Urbankova, Helena, Cools, Jan, Wlodarska, Iwona. 2014. Integrative genomic and transcriptomic analysis identified candidate genes implicated in the pathogenesis of hepatosplenic T-cell lymphoma. In PloS one, 9, e102977. doi:10.1371/journal.pone.0102977. https://pubmed.ncbi.nlm.nih.gov/25057852/
9. Zhang, Jia-Ning, Wei, Feng, Zheng, Bin Bin, Tang, Liang, Chen, Feng-Yuan. . Role of miRNA-99a-5p in Modulating the Function of Hepatocellular Carcinoma Cells: Bioinformatics Analysis and In Vitro Assay. In Current cancer drug targets, 23, 461-470. doi:10.2174/1568009623666230202155440. https://pubmed.ncbi.nlm.nih.gov/36733207/
10. Januchowski, Radosław, Sterzyńska, Karolina, Zawierucha, Piotr, Zabel, Maciej, Klejewski, Andrzej. . Microarray-based detection and expression analysis of new genes associated with drug resistance in ovarian cancer cell lines. In Oncotarget, 8, 49944-49958. doi:10.18632/oncotarget.18278. https://pubmed.ncbi.nlm.nih.gov/28611294/