Tekt2-flox 基因敲除小鼠

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产品名称

Tekt2-flox 基因敲除小鼠

产品编号

S-CKO-08306

品系全称

C57BL/6JCya-Tekt2em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-24084-Tekt2-B6J-VA

品系状态

使用本品系发表的文献需注明: Tekt2-flox 基因敲除小鼠 mice (Strain S-CKO-08306) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
tektin 2
基因别称
tektin-t
染色体号
Chr 4 (Mouse)
转录本 ID
NCBI: NM_001357262.1 | Ensembl: ENSMUST00000102616
修饰方式
条件性基因敲除
靶向范围
Exon 6~11
敲除长度
~2385 bp
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1346335Mice homozygous for a gene trapped allele exhibit male infertility and impaired motility of both sperm flagella and tracheal cilia due to altered dynein inner arm morphology and function.
TEKT2,全称为睾丸特异性激肽释放酶样蛋白2,是一种与精子发生相关的基因。TEKT2编码的蛋白质是精子鞭毛的结构成分,对精子鞭毛的稳定性和运动功能至关重要[5]。TEKT2的表达在精子发生过程中逐渐升高,并在成熟精子中达到高峰[7]。TEKT2基因的突变会导致精子鞭毛结构和功能的异常,从而引起男性不育[4]。TEKT2蛋白定位于精子鞭毛的外致密纤维(ODFs)表面,可能作为ODF相关分子参与鞭毛稳定性和精子运动[6]。

TEKT2的表达在多种疾病中发生变化。在糖尿病肾病(DN)患者中,TEKT2的表达升高,并且与足细胞骨架重塑和NPHS1蛋白下调有关[1]。在宫颈癌患者中,TEKT2的表达降低,并且与淋巴结转移和预后不良相关[2]。在阿尔茨海默病(AD)患者中,TEKT2的表达升高,并且与DNA损伤和修复通路相关[3]。

TEKT2的表达还受到多种因素的调控。例如,Tulp1蛋白可以转录激活TEKT2的启动子,而Oog1基因可以抑制TEKT2的表达[8,9]。此外,miRNA也可能通过靶向TEKT2的mRNA来调控其表达[4]。

综上所述,TEKT2是一种重要的精子发生相关基因,其表达受到多种因素的调控,并参与多种疾病的发病机制。TEKT2的研究有助于深入理解精子发生和疾病发生机制,为男性不育和疾病的治疗提供新的思路和策略。

参考文献:
1. Li, Yuanqing, Lin, Hongchun, Shu, Shuangshuang, Hu, Zhaoyong, Peng, Hui. . Integrative transcriptome analysis reveals TEKT2 and PIAS2 involvement in diabetic nephropathy. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36, e22592. doi:10.1096/fj.202200740RR. https://pubmed.ncbi.nlm.nih.gov/36251411/
2. Guo, Yilin, Wang, Lu, Xu, Zhen, Li, Rui, Zhao, Hu. 2023. Lymph node metastasis-related gene signature shows good performance in predicting prognosis and immune infiltration in cervical cancer. In Frontiers in oncology, 13, 1190251. doi:10.3389/fonc.2023.1190251. https://pubmed.ncbi.nlm.nih.gov/37427104/
3. Xue, J, Liu, J, Geng, M, He, H, Fan, J. . [Identification of potential hub genes of Alzheimer's disease by weighted gene co-expression network analysis]. In Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 41, 1752-1762. doi:10.12122/j.issn.1673-4254.2021.12.01. https://pubmed.ncbi.nlm.nih.gov/35012905/
4. Cao, Haiming, Wan, Zi, Wang, Fei, Li, Xiaofeng, Hou, Jianquan. 2021. Downregulation of KIF2C and TEKT2 is associated with male infertility and testicular carcinoma. In Aging, 13, 22898-22911. doi:10.18632/aging.203583. https://pubmed.ncbi.nlm.nih.gov/34591790/
5. Shimasaki, Sayaka, Yamamoto, Etsuko, Murayama, Emi, Inai, Tetsuichiro, Iida, Hiroshi. . Subcellular localization of Tektin2 in rat sperm flagellum. In Zoological science, 27, 755-61. doi:10.2108/zsj.27.755. https://pubmed.ncbi.nlm.nih.gov/20822404/
6. Jia, Danna, Gao, Pan, Lv, Yuexia, Liu, Mugen, Ren, Xiang. 2022. Tulp1 deficiency causes early-onset retinal degeneration through affecting ciliogenesis and activating ferroptosis in zebrafish. In Cell death & disease, 13, 962. doi:10.1038/s41419-022-05372-w. https://pubmed.ncbi.nlm.nih.gov/36396940/
7. Xiong, Zhaocheng, Zhang, Haihang, Huang, Ben, Shi, Deshun, Li, Xiangping. 2018. Expression pattern of prohibitin, capping actin protein of muscle Z-line beta subunit and tektin-2 gene in Murrah buffalo sperm and its relationship with sperm motility. In Asian-Australasian journal of animal sciences, 31, 1729-1737. doi:10.5713/ajas.18.0025. https://pubmed.ncbi.nlm.nih.gov/29642674/
8. Liu, Shuang, Bian, Yan-Chao, Wang, Wan-Lun, Xiao, Rui, Zhang, Chuan-Ling. 2023. Identification of hub genes associated with spermatogenesis by bioinformatics analysis. In Scientific reports, 13, 18435. doi:10.1038/s41598-023-45620-3. https://pubmed.ncbi.nlm.nih.gov/37891374/
9. Honda, Shinnosuke, Miki, Yuka, Miyamoto, Yuya, Imai, Hiroshi, Minami, Naojiro. 2018. Oocyte-specific gene Oog1 suppresses the expression of spermatogenesis-specific genes in oocytes. In The Journal of reproduction and development, 64, 297-301. doi:10.1262/jrd.2018-024. https://pubmed.ncbi.nlm.nih.gov/29731491/