Gpr75-flox 基因敲除小鼠

下单100%中奖,最高可得千元京东卡
复苏/繁育服务
产品名称

Gpr75-flox 基因敲除小鼠

产品编号

S-CKO-07944

品系全称

C57BL/6JCya-Gpr75em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-237716-Gpr75-B6J-VA

品系状态

使用本品系发表的文献需注明: Gpr75-flox 基因敲除小鼠 mice (Strain S-CKO-07944) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
G protein-coupled receptor 75
基因别称
-
染色体号
Chr 11 (Mouse)
转录本 ID
NCBI: NM_175490 | Ensembl: ENSMUST00000109430
修饰方式
条件性基因敲除
靶向范围
Exon 2
敲除长度
~2.0 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:2441843Mice homozygous for a knock-out allele exhibit a mild progressive retinal degeneration caused by loss of both M-cone and S-cone photoreceptor cells that is accompanied by oxidative stress. Another null allele shows increased anxiety-like behaviors and disrupted fear conditioning recall. Males are resistant age-related gain in body mass.
GPR75,也称为G蛋白偶联受体75,是一种重要的G蛋白偶联受体。GPR75在多种生物学过程中发挥作用,包括能量代谢、炎症反应、肿瘤发生和神经系统功能。GPR75的配体包括20-羟基二十碳四烯酸(20-HETE)和趋化因子CCL5等。GPR75通过激活下游的信号通路,影响细胞生长、分化和功能。

GPR75与肥胖和代谢综合征的发生发展密切相关。研究发现,GPR75基因的失功能突变与较低的体重指数和体重相关。GPR75敲除小鼠在高脂饮食下表现出抵抗体重增加和改善的血糖控制。此外,GPR75的激活与高血压、炎症、肥胖和胰岛素抵抗的发生发展相关。因此,GPR75是治疗肥胖和代谢综合征的重要靶点[1,2,3,4]。

在肿瘤发生中,GPR75也发挥着重要作用。研究发现,GPR75在前列腺癌细胞中表达上调,并与雄激素受体(AR)的表达和转录活性相关。GPR75的激活可以促进前列腺癌细胞的侵袭和转移。此外,GPR75还参与调节其他肿瘤的发生发展,如神经母细胞瘤和肝癌[5,6,7]。

GPR75在神经系统中也发挥着重要作用。研究发现,GPR75可以与趋化因子CCL5结合,激活下游的信号通路,保护神经元免受损伤。此外,GPR75还参与调节神经胶质细胞的活化和炎症反应,影响脑损伤的发生发展[8,9]。

综上所述,GPR75是一种重要的G蛋白偶联受体,在多种生物学过程中发挥作用。GPR75与肥胖、代谢综合征、肿瘤和神经系统疾病的发生发展密切相关。因此,GPR75是治疗这些疾病的重要靶点。未来研究可以进一步探索GPR75的调控机制和功能,为开发新的治疗策略提供理论基础。

参考文献:
1. Akbari, Parsa, Gilani, Ankit, Sosina, Olukayode, Ferreira, Manuel A R, Lotta, Luca A. . Sequencing of 640,000 exomes identifies GPR75 variants associated with protection from obesity. In Science (New York, N.Y.), 373, . doi:10.1126/science.abf8683. https://pubmed.ncbi.nlm.nih.gov/34210852/
2. Hossain, Sakib, Gilani, Ankit, Pascale, Jonathan, Garcia, Victor, Schwartzman, Michal Laniado. 2023. Gpr75-deficient mice are protected from high-fat diet-induced obesity. In Obesity (Silver Spring, Md.), 31, 1024-1037. doi:10.1002/oby.23692. https://pubmed.ncbi.nlm.nih.gov/36854900/
3. Fragner, Michael L, Parikh, Manish A, Jackson, Kaedrea A, Frishman, William H, Peterson, Stephen J. 2024. GPR75: A Newly Identified Receptor for Targeted Intervention in the Treatment of Obesity and Metabolic Syndrome. In Cardiology in review, , . doi:10.1097/CRD.0000000000000711. https://pubmed.ncbi.nlm.nih.gov/38695569/
4. Wang, Shuo, Gao, Shan, Wang, Fei. 2024. Effect and mechanism of GPR75 in metabolic dysfunction-related steatosis liver disease. In International journal of medical sciences, 21, 2343-2347. doi:10.7150/ijms.101094. https://pubmed.ncbi.nlm.nih.gov/39310267/
5. Froogh, Ghezal, Garcia, Victor, Laniado Schwartzman, Michal. 2022. The CYP/20-HETE/GPR75 axis in hypertension. In Advances in pharmacology (San Diego, Calif.), 94, 1-25. doi:10.1016/bs.apha.2022.02.003. https://pubmed.ncbi.nlm.nih.gov/35659370/
6. Cárdenas, Sofia, Colombero, Cecilia, Panelo, Laura, Costas, Monica A, Nowicki, Susana. 2019. GPR75 receptor mediates 20-HETE-signaling and metastatic features of androgen-insensitive prostate cancer cells. In Biochimica et biophysica acta. Molecular and cell biology of lipids, 1865, 158573. doi:10.1016/j.bbalip.2019.158573. https://pubmed.ncbi.nlm.nih.gov/31760076/
7. Cárdenas, Sofia, Colombero, Cecilia, Cruz, Mariana, Falck, John R, Nowicki, Susana. 2022. 20-HETE/GPR75 pairing modulates the expression and transcriptional activity of the androgen receptor in androgen-sensitive prostate cancer cells. In Molecular and cellular endocrinology, 559, 111784. doi:10.1016/j.mce.2022.111784. https://pubmed.ncbi.nlm.nih.gov/36202260/
8. Dedoni, Simona, Campbell, Lee A, Harvey, Brandon K, Avdoshina, Valeria, Mocchetti, Italo. 2018. The orphan G-protein-coupled receptor 75 signaling is activated by the chemokine CCL5. In Journal of neurochemistry, 146, 526-539. doi:10.1111/jnc.14463. https://pubmed.ncbi.nlm.nih.gov/29772059/
9. Ma, Zhihui, Ning, Yalei, Chen, Xiaoli, Li, Ping, Shu, Shiyu. 2024. 20-Hydroxyeicosatetraenoic Acid Regulates the Src/EGFR/NF-κB Signaling Pathway Via GPR75 to Activate Microglia and Promote TBI in the Immature Brain. In Neurochemical research, 50, 7. doi:10.1007/s11064-024-04260-3. https://pubmed.ncbi.nlm.nih.gov/39541047/