Serpini1-flox 基因敲除小鼠

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产品名称

Serpini1-flox 基因敲除小鼠

产品编号

S-CKO-05202

品系全称

C57BL/6JCya-Serpini1em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-20713-Serpini1-B6J-VA

品系状态

使用本品系发表的文献需注明: Serpini1-flox 基因敲除小鼠 mice (Strain S-CKO-05202) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
serine (or cysteine) peptidase inhibitor, clade I, member 1
基因别称
Ns,PI-12,PI12,Spi17
染色体号
Chr 3 (Mouse)
转录本 ID
NCBI: NM_009250 | Ensembl: ENSMUST00000029423
修饰方式
条件性基因敲除
靶向范围
Exon 2~3
敲除长度
~2.1 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1194506Mice homozygous for a disruption in this gene show no change in brain morphology or histology. However, they show an increase avoidance of novel stimuli and increased anxiety responses in some situations.
Serpini1,也称为神经丝蛋白,是一种编码丝氨酸蛋白酶抑制剂的基因。神经丝蛋白在神经系统中发挥重要作用,参与调节神经递质的释放和神经元的生长。此外,神经丝蛋白还在其他组织中表达,如肝脏、肺和肾脏,参与调节细胞生长、分化和凋亡。

Serpini1基因的表达受到多种因素的调控。研究表明,microRNA-21可以抑制Serpini1基因的表达,从而影响胃癌细胞的生长和增殖[1]。此外,Serpini1基因的表达还受到c-Myc转录因子的调控,c-Myc可以结合Serpini1基因的启动子区域,驱动基因的表达[4]。此外,Serpini1基因的表达还受到其他基因的调控,如PDCD10基因,两个基因通过一个不对称的双向启动子紧密相连,共同调控基因的表达[7]。

Serpini1基因的突变或异常表达与多种疾病的发生和发展相关。研究表明,Serpini1基因的突变可以导致家族性脑病,这是一种罕见的神经退行性疾病,特征为神经丝蛋白包涵体的形成[3][9]。此外,Serpini1基因的表达异常还与焦虑、结肠癌、肝细胞癌和膀胱癌等疾病相关[2][5][6][8]。研究表明,Serpini1基因的表达异常可以影响细胞的增殖、分化和凋亡,从而促进肿瘤的发生和发展。

综上所述,Serpini1基因是一种重要的基因,参与调节神经系统的发育和功能,并在多种疾病的发生和发展中发挥重要作用。Serpini1基因的表达受到多种因素的调控,包括microRNA、转录因子和其他基因的调控。Serpini1基因的突变或异常表达与多种疾病相关,包括家族性脑病、焦虑、结肠癌、肝细胞癌和膀胱癌等。因此,深入研究Serpini1基因的功能和调控机制,对于理解相关疾病的发生和发展具有重要意义。

参考文献:
1. Yamanaka, Sumitaka, Olaru, Alexandru V, An, Fangmei, Meltzer, Stephen J, Selaru, Florin M. 2012. MicroRNA-21 inhibits Serpini1, a gene with novel tumour suppressive effects in gastric cancer. In Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, 44, 589-96. doi:10.1016/j.dld.2012.02.016. https://pubmed.ncbi.nlm.nih.gov/22464652/
2. Han, Sha, Fei, Fei, Sun, Shaoyang, Wang, Xu, Wang, Liang. 2020. Increased anxiety was found in serpini1 knockout zebrafish larval. In Biochemical and biophysical research communications, 534, 1013-1019. doi:10.1016/j.bbrc.2020.10.048. https://pubmed.ncbi.nlm.nih.gov/33168193/
3. Yang, Xiaoyue, Fang, Zhixu, Yan, Lisi, Cheng, Min, Jiang, Li. 2022. Role of SERPINI1 pathogenic variants in familial encephalopathy with neuroserpin inclusion bodies: A case report and literature review. In Seizure, 103, 137-147. doi:10.1016/j.seizure.2022.11.008. https://pubmed.ncbi.nlm.nih.gov/36417830/
4. Chen, Ping-Yen, Chang, Wun-Shaing W, Lai, Yiu-Kay, Wu, Cheng-Wen. 2009. c-Myc regulates the coordinated transcription of brain disease-related PDCD10-SERPINI1 bidirectional gene pair. In Molecular and cellular neurosciences, 42, 23-32. doi:10.1016/j.mcn.2009.05.001. https://pubmed.ncbi.nlm.nih.gov/19442737/
5. Matsuda, Yasufumi, Miura, Koh, Yamane, Junko, Naitoh, Takeshi, Unno, Michiaki. 2016. SERPINI1 regulates epithelial-mesenchymal transition in an orthotopic implantation model of colorectal cancer. In Cancer science, 107, 619-28. doi:10.1111/cas.12909. https://pubmed.ncbi.nlm.nih.gov/26892864/
6. Han, Yawei, Wang, Gaoyv, Han, Erwei, Li, Yueguo, Ren, Li. 2025. SERPINI1 serves as a biomarker promoting cell proliferation and invasion in hepatocellular carcinoma. In Cancer cell international, 25, 88. doi:10.1186/s12935-025-03716-y. https://pubmed.ncbi.nlm.nih.gov/40082896/
7. Chen, Ping-Yen, Chang, Wun-Shaing W, Chou, Ruey-Hwang, Chi, Chia-Yi, Wu, Cheng-Wen. 2007. Two non-homologous brain diseases-related genes, SERPINI1 and PDCD10, are tightly linked by an asymmetric bidirectional promoter in an evolutionarily conserved manner. In BMC molecular biology, 8, 2. doi:. https://pubmed.ncbi.nlm.nih.gov/17212813/
8. Zhang, Yongqing, Chen, Qingyuan, Gong, Meiqin, Zeng, Yuanqi, Gao, Dongrui. 2021. Gene regulatory networks analysis of muscle-invasive bladder cancer subtypes using differential graphical model. In BMC genomics, 22, 863. doi:10.1186/s12864-021-08113-z. https://pubmed.ncbi.nlm.nih.gov/34852762/
9. Ranza, Emmanuelle, Garcia-Tarodo, Stephanie, Varvagiannis, Konstantinos, Fluss, Joel, Korff, Christian M. 2017. SERPINI1 pathogenic variants: An emerging cause of childhood-onset progressive myoclonic epilepsy. In American journal of medical genetics. Part A, 173, 2456-2460. doi:10.1002/ajmg.a.38317. https://pubmed.ncbi.nlm.nih.gov/28631894/