Pip5k1a-flox 基因敲除小鼠

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产品名称

Pip5k1a-flox 基因敲除小鼠

产品编号

S-CKO-04287

品系全称

C57BL/6JCya-Pip5k1aem1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-18720-Pip5k1a-B6J-VA

品系状态

使用本品系发表的文献需注明: Pip5k1a-flox 基因敲除小鼠 mice (Strain S-CKO-04287) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha
基因别称
PI4P5K-I[a],PIP5K1-alpha,PIP5KIalpha,Pipk5a
染色体号
Chr 3 (Mouse)
转录本 ID
NCBI: NM_001293707 | Ensembl: ENSMUST00000107233
修饰方式
条件性基因敲除
靶向范围
Exon 5~7
敲除长度
~2.8 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:107929A gene trap insertion into an intron of this gene results in no obvious phenotype. Mice homozygous for a knock-out allele exhibit partial lethality and reduced male fertility associated with asthenozoospermia and abnormal midpiece morphology.
PIPK1A,也称为磷脂酰肌醇-4-磷酸 5-激酶1A,是一种重要的磷脂酰肌醇激酶。磷脂酰肌醇激酶是一类调节磷脂酰肌醇信号通路的酶,参与调控细胞生长、分化、凋亡和肿瘤发生等生物学过程。PIPK1A通过催化磷脂酰肌醇-4-磷酸(PI4P)生成磷脂酰肌醇-4,5-二磷酸(PI(4,5)P2)和磷脂酰肌醇-3,4,5-三磷酸(PI(3,4,5)P3),在细胞信号转导中发挥重要作用。

PIPK1A在多种疾病中发挥重要作用。例如,在乳腺癌中,PIPK1A基因的扩增与肿瘤的发生和发展密切相关[5]。此外,PIPK1A还与结肠癌、子宫内膜异位症和肥胖相关疾病的发生和发展有关[7,8,9]。研究表明,PIPK1A的异常表达和功能改变可能参与了这些疾病的病理生理过程。

PIPK1A的表达和功能也受到多种因素的调节。例如,褪黑激素(MT)可以上调PIPK1A的表达,促进皮肤成纤维细胞的增殖[1]。此外,PIPK1A还可以与KRAS基因相互作用,参与KRAS信号通路的调节,影响肿瘤的发生和发展[4]。此外,PIPK1A还可以与miRNA相互作用,影响基因表达和细胞功能[6,7,8]。

PIPK1A在细胞内的定位和功能也受到多种因素的调节。例如,PIPK1A主要表达于皮肤毛囊的外根鞘[1]。此外,PIPK1A还可以与YAP/TAZ转录因子相互作用,影响基因表达和细胞功能[3]。此外,PIPK1A还可以与多种蛋白质相互作用,形成复合物,参与细胞信号转导和基因表达调控[2,4]。

综上所述,PIPK1A是一种重要的磷脂酰肌醇激酶,参与调控细胞生长、分化、凋亡和肿瘤发生等生物学过程。PIPK1A在多种疾病中发挥重要作用,包括乳腺癌、结肠癌、子宫内膜异位症和肥胖相关疾病。此外,PIPK1A的表达和功能也受到多种因素的调节,例如褪黑激素、KRAS基因和miRNA等。PIPK1A的研究有助于深入理解磷脂酰肌醇信号通路的生物学功能和疾病发生机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Jin, Mei, Fan, Weiyu, Xue, Tianwei, Cong, Linlin. 2025. Functional analysis of the PIP5K1A gene in Liaoning Cashmere goats: an investigation based on bioinformatics, tissue localization, and biological functions. In Cellular and molecular biology (Noisy-le-Grand, France), 70, 45-51. doi:10.14715/cmb/2024.70.12.6. https://pubmed.ncbi.nlm.nih.gov/39799498/
2. Babushok, Daria V, Ohshima, Kazuhiko, Ostertag, Eric M, Abrams, Charles S, Kazazian, Haig H. 2007. A novel testis ubiquitin-binding protein gene arose by exon shuffling in hominoids. In Genome research, 17, 1129-38. doi:. https://pubmed.ncbi.nlm.nih.gov/17623810/
3. Jung, Oisun, Baek, Min-Jeong, Wooldrik, Colin, Anderson, Richard A, Choi, Suyong. 2024. Nuclear phosphoinositide signaling promotes YAP/TAZ-TEAD transcriptional activity in breast cancer. In The EMBO journal, 43, 1740-1769. doi:10.1038/s44318-024-00085-6. https://pubmed.ncbi.nlm.nih.gov/38565949/
4. Adhikari, Hema, Counter, Christopher M. 2018. Interrogating the protein interactomes of RAS isoforms identifies PIP5K1A as a KRAS-specific vulnerability. In Nature communications, 9, 3646. doi:10.1038/s41467-018-05692-6. https://pubmed.ncbi.nlm.nih.gov/30194290/
5. Waugh, Mark G. 2014. Amplification of Chromosome 1q Genes Encoding the Phosphoinositide Signalling Enzymes PI4KB, AKT3, PIP5K1A and PI3KC2B in Breast Cancer. In Journal of Cancer, 5, 790-6. doi:10.7150/jca.9794. https://pubmed.ncbi.nlm.nih.gov/25368680/
6. Murphy, Andrew, Vyavahare, Sagar, Kumar, Sandeep, Isales, Carlos M, Fulzele, Sadanand. 2022. Dietary interventions and molecular mechanisms for healthy musculoskeletal aging. In Biogerontology, 23, 681-698. doi:10.1007/s10522-022-09970-1. https://pubmed.ncbi.nlm.nih.gov/35727468/
7. Zhang, Qu, Zhang, Chi, Ma, Jian-Xin, Sun, Yu, Xu, Jiao-Zhen. . Circular RNA PIP5K1A promotes colon cancer development through inhibiting miR-1273a. In World journal of gastroenterology, 25, 5300-5309. doi:10.3748/wjg.v25.i35.5300. https://pubmed.ncbi.nlm.nih.gov/31558874/
8. Sun, Lin, Wei, Yan, Wang, Junli. . Circular RNA PIP5K1A (circPIP5K1A) accelerates endometriosis progression by regulating the miR-153-3p/Thymosin Beta-4 X-Linked (TMSB4X) pathway. In Bioengineered, 12, 7104-7118. doi:10.1080/21655979.2021.1978618. https://pubmed.ncbi.nlm.nih.gov/34546850/
9. Doumatey, Ayo P, Xu, Huichun, Huang, Hanxia, Adeyemo, Adebowale, Rotimi, Charles N. . Global Gene Expression Profiling in Omental Adipose Tissue of Morbidly Obese Diabetic African Americans. In Journal of endocrinology and metabolism, 5, 199-210. doi:. https://pubmed.ncbi.nlm.nih.gov/26504501/