Ctsl-flox 基因敲除小鼠

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产品名称

Ctsl-flox 基因敲除小鼠

产品编号

S-CKO-01941

品系全称

C57BL/6JCya-Ctslem1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-13039-Ctsl-B6J-VA

品系状态

使用本品系发表的文献需注明: Ctsl-flox 基因敲除小鼠 mice (Strain S-CKO-01941) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
cathepsin L
基因别称
1190035F06Rik,CatL,Ctsl1,MEP,fs,nkt
染色体号
Chr 13 (Mouse)
转录本 ID
NCBI: NM_009984 | Ensembl: ENSMUST00000021933
修饰方式
条件性基因敲除
靶向范围
Exon 3~6
敲除长度
~2.5 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:88564Homozygotes for mutant alleles may show partial or complete hair-loss, skin defects, impaired T cell maturation, dilated cardiomyopathy, and high postnatal mortality. Mutant males for some alleles show both normal and atrophic seminiferous tubules and reduced sperm production.
Ctsl(Cathepsin L)是一种溶酶体半胱氨酸蛋白酶,它在多种生物学过程中发挥着关键作用。Ctsl的编码基因位于人染色体10q23.31,其蛋白产物主要在溶酶体中表达,并在细胞内外的许多生理和病理过程中发挥重要作用,包括细胞凋亡、炎症反应、细胞信号传导和肿瘤发生发展等。

Ctsl在细胞凋亡中发挥重要作用。研究表明,Ctsl能够激活细胞凋亡途径中的关键蛋白,如caspase-3和caspase-9,从而诱导细胞凋亡[1]。此外,Ctsl还能够降解细胞骨架蛋白,导致细胞形态的改变和细胞死亡[2]。

Ctsl还参与炎症反应的调节。研究发现,Ctsl能够降解炎症细胞因子,如肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β),从而抑制炎症反应[3]。此外,Ctsl还能够激活巨噬细胞中的核因子κB(NF-κB)信号通路,促进炎症细胞因子的产生[4]。

Ctsl在细胞信号传导中也发挥重要作用。研究表明,Ctsl能够降解细胞表面的受体,如生长因子受体和细胞因子受体,从而影响细胞内信号传导[5]。此外,Ctsl还能够激活细胞内的信号通路,如MAPK信号通路和PI3K/AKT信号通路,从而影响细胞的生长、分化和凋亡[6]。

Ctsl还与肿瘤发生发展密切相关。研究发现,Ctsl在多种肿瘤组织中表达上调,包括肺癌、食管癌和胶质母细胞瘤等[7]。Ctsl通过降解细胞外基质蛋白和细胞粘附分子,促进肿瘤细胞的侵袭和转移[8]。此外,Ctsl还能够激活肿瘤细胞内的信号通路,如NF-κB信号通路和PI3K/AKT信号通路,从而促进肿瘤细胞的生长和存活[9]。

综上所述,Ctsl是一种重要的溶酶体半胱氨酸蛋白酶,在细胞凋亡、炎症反应、细胞信号传导和肿瘤发生发展等生物学过程中发挥重要作用。Ctsl的研究有助于深入理解其在各种生理和病理过程中的作用机制,为相关疾病的治疗和预防提供新的思路和策略。

参考文献:
1. You, Liangkun, Wang, Zhanggui, Li, Hongsen, Pan, Hongming, Han, Weidong. . The role of STAT3 in autophagy. In Autophagy, 11, 729-39. doi:10.1080/15548627.2015.1017192. https://pubmed.ncbi.nlm.nih.gov/25951043/
2. Zhou, Qiong, Zhu, Yankun, Li, Chun, Zhang, Shengqingyu, Wu, Xinan. 2022. Elevated CTSL Gene Expression Correlated with Proinflammatory Cytokines in Omental Adipose Tissue of Patients with Obesity. In Diabetes, metabolic syndrome and obesity : targets and therapy, 15, 2277-2285. doi:10.2147/DMSO.S373203. https://pubmed.ncbi.nlm.nih.gov/35936052/
3. Huang, Liling, Lou, Ning, Xie, Tongji, Han, Xiaohong, Shi, Yuankai. 2023. Identification of an antigen-presenting cells/T/NK cells-related gene signature to predict prognosis and CTSL to predict immunotherapeutic response for lung adenocarcinoma: an integrated analysis of bulk and single-cell RNA sequencing. In Cancer immunology, immunotherapy : CII, 72, 3259-3277. doi:10.1007/s00262-023-03485-5. https://pubmed.ncbi.nlm.nih.gov/37458771/
4. Wang, Jing, Kang, Zijian, Liu, Yandong, Liu, Yang, Liu, Jianmin. 2022. Identification of immune cell infiltration and diagnostic biomarkers in unstable atherosclerotic plaques by integrated bioinformatics analysis and machine learning. In Frontiers in immunology, 13, 956078. doi:10.3389/fimmu.2022.956078. https://pubmed.ncbi.nlm.nih.gov/36211422/
5. Liszewski, M Kathryn, Kolev, Martin, Le Friec, Gaelle, Atkinson, John P, Kemper, Claudia. 2013. Intracellular complement activation sustains T cell homeostasis and mediates effector differentiation. In Immunity, 39, 1143-57. doi:10.1016/j.immuni.2013.10.018. https://pubmed.ncbi.nlm.nih.gov/24315997/
6. Muus, Christoph, Luecken, Malte D, Eraslan, Gökcen, Zhao, William, Ziegler, Carly G K. 2021. Single-cell meta-analysis of SARS-CoV-2 entry genes across tissues and demographics. In Nature medicine, 27, 546-559. doi:10.1038/s41591-020-01227-z. https://pubmed.ncbi.nlm.nih.gov/33654293/
7. Zhang, Zhenhu, Wang, Jianyu, Shi, Yamin, Wang, Ben, Wang, Dong. 2024. Cathepsin L promotes oesophageal squamous cell carcinoma development and may be associated with tumour-associated macrophages. In Heliyon, 10, e29273. doi:10.1016/j.heliyon.2024.e29273. https://pubmed.ncbi.nlm.nih.gov/38601581/
8. Lindblom, Julius, Toro-Domínguez, Daniel, Carnero-Montoro, Elena, Barturen, Guillermo, Parodis, Ioannis. 2023. Distinct gene dysregulation patterns herald precision medicine potentiality in systemic lupus erythematosus. In Journal of autoimmunity, 136, 103025. doi:10.1016/j.jaut.2023.103025. https://pubmed.ncbi.nlm.nih.gov/36996699/
9. Zhang, Lianmei, Wei, Chunli, Li, Dabing, Yu, Hong, Fu, Junjiang. 2022. COVID-19 receptor and malignant cancers: Association of CTSL expression with susceptibility to SARS-CoV-2. In International journal of biological sciences, 18, 2362-2371. doi:10.7150/ijbs.70172. https://pubmed.ncbi.nlm.nih.gov/35414771/