Crmp1-flox 基因敲除小鼠

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产品名称

Crmp1-flox 基因敲除小鼠

产品编号

S-CKO-01869

品系全称

C57BL/6JCya-Crmp1em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-12933-Crmp1-B6J-VA

品系状态

使用本品系发表的文献需注明: Crmp1-flox 基因敲除小鼠 mice (Strain S-CKO-01869) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
collapsin response mediator protein 1
基因别称
CRMP-1,DRP-1,Dpysl1,ULIP-3,Ulip3
染色体号
Chr 5 (Mouse)
转录本 ID
NCBI: NM_001136058.2 | Ensembl: ENSMUST00000114158
修饰方式
条件性基因敲除
靶向范围
Exon 7~8
敲除长度
~1417 bp
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:107793Mice homozygous for one knock-out allele show transient postnatal changes in granule cell proliferation, apoptosis and migration in cerebellum and delayed radial migration of cortical neurons in cerebral cortex. Homozygotes for another knock-out allele show reduced LTP and impaired spatial learning.
CRMP1,即Collapsin Response Mediator Protein 1,是一种重要的细胞质蛋白,参与调节中枢神经系统的发育。CRMP1属于细胞质CRMP家族蛋白,与神经元发育和轴突生长密切相关。CRMP1在多种生物学过程中发挥作用,包括细胞分化、发育、代谢和疾病发生。

在神经发育方面,CRMP1对神经元轴突生长和树突发育具有重要作用。研究发现,CRMP1在视网膜神经节细胞(RGCs)中表达,并且在损伤后促进RGC轴突再生和细胞存活[2]。此外,CRMP1和CRMP2协同作用,通过重叠和独特的信号通路,调节树突棘成熟和模式[4]。在发育中的小脑中,CRMP1和CRMP2的缺失会导致浦肯野细胞迁移缺陷[5]。

在神经系统疾病方面,CRMP1基因的变异与神经发育障碍有关。研究发现,CRMP1基因的单倍型变异在患有肌肉低张力、智力障碍和/或自闭症谱系障碍的个体中被发现[1]。此外,CRMP1的表达水平在精神疾病中发生了改变[1]。

在肿瘤生物学方面,CRMP1作为侵袭和转移抑制因子发挥作用。研究发现,CRMP1在前列腺癌组织中表达下调,并且其下调与上皮-间质转化(EMT)和细胞骨架重排有关[7]。此外,CRMP1的表达与乳腺癌腋窝淋巴结转移相关,表明其可能在乳腺癌转移中发挥作用[8]。

CRMP1还在其他生物学过程中发挥作用。例如,研究发现,CRMP1在髓母细胞瘤中抑制增殖,并且受高迁移率组蛋白A1(HMGA1)的调控[3]。此外,CRMP1还与埃利斯-范克雷夫尔德(EVC)综合征的严重程度相关,表明其可能是EVC综合征的遗传修饰因子[6]。

综上所述,CRMP1是一种重要的细胞质蛋白,参与调节中枢神经系统的发育和功能。CRMP1在多种生物学过程中发挥作用,包括神经发育、神经系统疾病和肿瘤生物学。CRMP1的研究有助于深入理解神经系统的发育和功能,以及疾病的发生机制。

参考文献:
1. Ravindran, Ethiraj, Arashiki, Nobuto, Becker, Lena-Luise, Nakamura, Fumio, Kaindl, Angela M. 2022. Monoallelic CRMP1 gene variants cause neurodevelopmental disorder. In eLife, 11, . doi:10.7554/eLife.80793. https://pubmed.ncbi.nlm.nih.gov/36511780/
2. Lukomska, Agnieszka, Theune, William C, Xing, Jian, Gupta, Mahit, Trakhtenberg, Ephraim F. 2023. Experimental gene expression of developmentally downregulated Crmp1, Crmp4, and Crmp5 promotes axon regeneration and retinal ganglion cell survival after optic nerve injury. In Brain research, 1809, 148368. doi:10.1016/j.brainres.2023.148368. https://pubmed.ncbi.nlm.nih.gov/37059258/
3. Li, Kay Ka-Wai, Qi, Yan, Xia, Tian, Lau, Kin-Mang, Ng, Ho-Keung. 2015. CRMP1 Inhibits Proliferation of Medulloblastoma and Is Regulated by HMGA1. In PloS one, 10, e0127910. doi:10.1371/journal.pone.0127910. https://pubmed.ncbi.nlm.nih.gov/26009886/
4. Makihara, Hiroko, Nakai, Shiori, Ohkubo, Wataru, Kolattukudy, Pappachan, Goshima, Yoshio. 2016. CRMP1 and CRMP2 have synergistic but distinct roles in dendritic development. In Genes to cells : devoted to molecular & cellular mechanisms, 21, 994-1005. doi:10.1111/gtc.12399. https://pubmed.ncbi.nlm.nih.gov/27480924/
5. Akinaga, Satoshi, Harada, Sayaka, Takahashi, Miyuki, Goshima, Yoshio, Ohshima, Toshio. 2022. Loss of CRMP1 and CRMP2 results in migration defects of Purkinje cells in the X lobule of the mouse cerebellum. In Brain research, 1783, 147846. doi:10.1016/j.brainres.2022.147846. https://pubmed.ncbi.nlm.nih.gov/35219721/
6. Da Silva, Jorge Diogo, Soares, Ana Rita, Fortuna, Ana Maria, Tkachenko, Nataliya. 2023. Establishing an objective clinical spectrum, genotype-phenotype correlations, and CRMP1 as a modifier in the Ellis-van Creveld syndrome: The first systematic review of EVC- and EVC2-associated conditions. In Genetics in medicine open, 1, 100781. doi:10.1016/j.gimo.2023.100781. https://pubmed.ncbi.nlm.nih.gov/39669252/
7. Cai, G, Wu, D, Wang, Z, Chan, F L, Yu, S. 2016. Collapsin response mediator protein-1 (CRMP1) acts as an invasion and metastasis suppressor of prostate cancer via its suppression of epithelial-mesenchymal transition and remodeling of actin cytoskeleton organization. In Oncogene, 36, 546-558. doi:10.1038/onc.2016.227. https://pubmed.ncbi.nlm.nih.gov/27321179/
8. Luo, Jianguo, Chen, Shaojun, Chen, Jingsen, He, Fei, Wang, Enli. 2022. Identification and validation of DNA methylation markers to predict axillary lymph node metastasis of breast cancer. In PloS one, 17, e0278270. doi:10.1371/journal.pone.0278270. https://pubmed.ncbi.nlm.nih.gov/36454866/