Prdx6-flox 基因敲除小鼠

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产品名称

Prdx6-flox 基因敲除小鼠

产品编号

S-CKO-01215

品系全称

C57BL/6JCya-Prdx6em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-11758-Prdx6-B6J-VA

品系状态

使用本品系发表的文献需注明: Prdx6-flox 基因敲除小鼠 mice (Strain S-CKO-01215) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
peroxiredoxin 6
基因别称
1-Cys Prx,1-cysPrx,9430088D19Rik,Aop2,Brp-12,CP-3,GPx,LPCAT-5,Ltw-4,Ltw4,Lvtw-4,NSGPx,ORF06,Prdx5,aiPLA2
染色体号
Chr 1 (Mouse)
转录本 ID
NCBI: NM_007453 | Ensembl: ENSMUST00000071718
修饰方式
条件性基因敲除
靶向范围
Exon 2
敲除长度
~0.8 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:894320Mice homozygous for disruptions of this gene show no macroscopic or microscopic abnormalities. However, they have an increased susceptibility to oxidative stress.
Prdx6,也称为Peroxiredoxin 6,是一种双功能蛋白质,具有磷脂酶A2(PLA2)和谷胱甘肽过氧化物酶(GPx)活性。它属于过氧化物酶家族,参与细胞内氧化还原平衡的维持,对抗氧化应激,对细胞的保护具有重要意义。Prdx6在多种组织中均有表达,包括脑、心脏、肝脏和肌肉等,参与多种生物学过程,如细胞信号传导、炎症反应和细胞凋亡等。

研究表明,Prdx6基因多态性与多种疾病的发生发展密切相关。例如,在系统性红斑狼疮(SLE)患者中,Prdx6-AS1基因的多态性与SLE的易感性显著相关[1]。此外,Prdx6基因多态性还与慢性阻塞性肺病(COPD)的易感性有关[2]。在猪的肌肉组织中,Prdx6基因的表达受到C/EBPβ和CREB的调控,与肌肉品质相关[3]。在衰老过程中,miR-24和其靶基因Prdx6的表达水平变化与肌肉再生能力下降有关[4]。Prdx6还参与调节Nlrp3炎症小体的活化,对维持细胞健康具有重要意义[5]。

Prdx6在肿瘤发生发展中也发挥着重要作用。例如,Prdx6基因敲除可以抑制肝内胆管癌(ICC)的恶性进展[6]。在肺腺癌中,Prdx6基因的表达水平升高,与患者预后不良相关[7]。Prdx6基因敲除小鼠表现出焦虑样行为、增强的情境恐惧记忆和空间记忆受损[8]。

综上所述,Prdx6是一种重要的双功能蛋白质,参与细胞内氧化还原平衡的维持,对抗氧化应激,对细胞的保护具有重要意义。Prdx6在多种生物学过程中发挥作用,如细胞信号传导、炎症反应和细胞凋亡等。Prdx6基因多态性与多种疾病的发生发展密切相关,包括SLE、COPD和肿瘤等。Prdx6的研究有助于深入理解其在生物学过程中的作用和疾病发生机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Zhang, Xiao-Xue, You, Jun-Peng, Liu, Xin-Ran, Zhao, Zhan-Zheng, Qi, Yuan-Yuan. 2022. PRDX6 AS1 gene polymorphisms and SLE susceptibility in Chinese populations. In Frontiers in immunology, 13, 987385. doi:10.3389/fimmu.2022.987385. https://pubmed.ncbi.nlm.nih.gov/36311744/
2. Xiong, Mingmei, Guo, Meihua, Huang, Dongjian, Li, Jing, Zhou, Yan. 2023. Effect of PRDX6 gene polymorphism on susceptibility to chronic obstructive pulmonary disease in the Chinese Han population. In The clinical respiratory journal, 17, 638-646. doi:10.1111/crj.13648. https://pubmed.ncbi.nlm.nih.gov/37329238/
3. Wu, Xinyu, Ji, Panlong, Zhang, Liang, Xiong, Yuanzhu, Zuo, Bo. 2015. The Expression of Porcine Prdx6 Gene Is Up-Regulated by C/EBPβ and CREB. In PloS one, 10, e0144851. doi:10.1371/journal.pone.0144851. https://pubmed.ncbi.nlm.nih.gov/26659441/
4. Soriano-Arroquia, Ana, Gostage, John, Xia, Qin, McDonagh, Brian, Goljanek-Whysall, Katarzyna. 2021. miR-24 and its target gene Prdx6 regulate viability and senescence of myogenic progenitors during aging. In Aging cell, 20, e13475. doi:10.1111/acel.13475. https://pubmed.ncbi.nlm.nih.gov/34560818/
5. Chhunchha, Bhavana, Kumar, Rakesh, Kubo, Eri, Thakur, Priyanka, Singh, Dhirendra P. 2023. Prdx6 Regulates Nlrp3 Inflammasome Activation-Driven Inflammatory Response in Lens Epithelial Cells. In International journal of molecular sciences, 24, . doi:10.3390/ijms242216276. https://pubmed.ncbi.nlm.nih.gov/38003466/
6. Min, Yoon, Kim, Mi-Jeong, Lee, Sena, Chun, Eunyoung, Lee, Ki-Young. 2018. Inhibition of TRAF6 ubiquitin-ligase activity by PRDX1 leads to inhibition of NFKB activation and autophagy activation. In Autophagy, 14, 1347-1358. doi:10.1080/15548627.2018.1474995. https://pubmed.ncbi.nlm.nih.gov/29929436/
7. Li, Hong, Wu, Zhengsheng, Zhong, Rulei, Chen, Qixin, Shen, Yuxian. 2022. PRDX6 knockout restrains the malignant progression of intrahepatic cholangiocarcinoma. In Medical oncology (Northwood, London, England), 39, 250. doi:10.1007/s12032-022-01822-9. https://pubmed.ncbi.nlm.nih.gov/36209344/
8. Chen, Zixin, Lin, Junjun, Wang, Huifang, Wang, Jing, Zhang, Zhou. . Expression and clinical role of PRDX6 in lung adenocarcinoma. In The Journal of international medical research, 52, 3000605241236276. doi:10.1177/03000605241236276. https://pubmed.ncbi.nlm.nih.gov/38506348/