Rfc4-flox 基因敲除小鼠

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产品名称

Rfc4-flox 基因敲除小鼠

产品编号

S-CKO-00499

品系全称

C57BL/6JCya-Rfc4em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-106344-Rfc4-B6J-VA

品系状态

使用本品系发表的文献需注明: Rfc4-flox 基因敲除小鼠 mice (Strain S-CKO-00499) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
replication factor C (activator 1) 4
基因别称
A1,RFC37
染色体号
Chr 16 (Mouse)
转录本 ID
NCBI: NM_145480.1 | Ensembl: ENSMUST00000023598
修饰方式
条件性基因敲除
靶向范围
Exon 3
敲除长度
~579 bp
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
RFC4,也称为复制因子C亚基4,是一种在DNA复制过程中发挥重要作用的蛋白质。RFC4是复制因子C复合体(Replication Factor C, RFC)的一个组成部分,该复合体负责将增殖细胞核抗原(PCNA)加载到DNA上,从而促进DNA聚合酶的进程性和DNA复制[6]。RFC4的缺失会导致细胞周期进程和DNA复制受损,并可能引发多种疾病[5]。

RFC4在癌症中扮演着复杂的角色。研究表明,RFC4在多种癌症中表达上调,并与肿瘤的发生、发展和预后密切相关[1,2,3,7,9]。例如,RFC4在非小细胞肺癌(NSCLC)中表达上调,并与Notch1信号通路相互作用,形成一个正反馈回路,促进肿瘤的转移和自我更新[1]。此外,RFC4还与宫颈癌、鼻咽癌和口腔舌鳞状细胞癌的发生和发展密切相关[2,4,7]。研究表明,RFC4的敲低可以抑制肿瘤细胞的增殖和转移,为癌症的治疗提供新的思路和策略[1,2,4,7]。

除了在癌症中的作用,RFC4还与多种疾病相关。研究表明,RFC4的缺失与一种多系统疾病相关,表现为运动失调、肌肉无力、听力丧失和体重下降[5]。此外,RFC4还与2型糖尿病相关,其表达水平与糖尿病的发生和发展密切相关[8]。

综上所述,RFC4是一种在DNA复制和多种生物学过程中发挥重要作用的蛋白质。RFC4在癌症、多系统疾病和2型糖尿病中发挥重要作用,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Liu, Lei, Tao, Tianyu, Liu, Shihua, Cai, Junchao, Tian, Han. 2021. An RFC4/Notch1 signaling feedback loop promotes NSCLC metastasis and stemness. In Nature communications, 12, 2693. doi:10.1038/s41467-021-22971-x. https://pubmed.ncbi.nlm.nih.gov/33976158/
2. Zhang, Jianwei, Meng, Silu, Wang, Xiaoyan, Ma, Ding, Li, Shuang. 2022. Sequential gene expression analysis of cervical malignant transformation identifies RFC4 as a novel diagnostic and prognostic biomarker. In BMC medicine, 20, 437. doi:10.1186/s12916-022-02630-8. https://pubmed.ncbi.nlm.nih.gov/36352434/
3. Yu, Lei, Li, Jing, Zhang, Mingyang, Yan, Jia, Wang, Changshan. . Identification of RFC4 as a potential biomarker for pan-cancer involving prognosis, tumour immune microenvironment and drugs. In Journal of cellular and molecular medicine, 28, e18478. doi:10.1111/jcmm.18478. https://pubmed.ncbi.nlm.nih.gov/39031628/
4. Guan, Shuzhen, Feng, Lin, Wei, Jinrui, Wang, Guizhen, Wu, Lichuan. 2022. Knockdown of RFC4 inhibits the cell proliferation of nasopharyngeal carcinoma in vitro and in vivo. In Frontiers of medicine, 17, 132-142. doi:10.1007/s11684-022-0938-x. https://pubmed.ncbi.nlm.nih.gov/36562948/
5. Morimoto, Marie, Ryu, Eunjin, Steger, Benjamin J, Myung, Kyungjae, Malicdan, May Christine V. 2024. Expanding the genetic and phenotypic landscape of replication factor C complex-related disorders: RFC4 deficiency is linked to a multisystemic disorder. In American journal of human genetics, 111, 1970-1993. doi:10.1016/j.ajhg.2024.07.008. https://pubmed.ncbi.nlm.nih.gov/39106866/
6. Li, X, Burgers, P M. . Cloning and characterization of the essential Saccharomyces cerevisiae RFC4 gene encoding the 37-kDa subunit of replication factor C. In The Journal of biological chemistry, 269, 21880-4. doi:. https://pubmed.ncbi.nlm.nih.gov/8063832/
7. Zhang, Jian, Wang, Linlin, Xie, Xiao. 2021. RFC4 promotes the progression and growth of Oral Tongue squamous cell carcinoma in vivo and vitro. In Journal of clinical laboratory analysis, 35, e23761. doi:10.1002/jcla.23761. https://pubmed.ncbi.nlm.nih.gov/33783864/
8. Che, Xuanqiang, Zhao, Ran, Xu, Hua, Zhao, Shumiao, Ma, Hongwei. 2019. Differently Expressed Genes (DEGs) Relevant to Type 2 Diabetes Mellitus Identification and Pathway Analysis via Integrated Bioinformatics Analysis. In Medical science monitor : international medical journal of experimental and clinical research, 25, 9237-9244. doi:10.12659/MSM.918407. https://pubmed.ncbi.nlm.nih.gov/31797865/
9. Wang, Jing, Luo, Fei-Fei, Huang, Tie-Jun, Qian, Chao-Nan, Huang, Bi-Jun. 2021. The upregulated expression of RFC4 and GMPS mediated by DNA copy number alteration is associated with the early diagnosis and immune escape of ESCC based on a bioinformatic analysis. In Aging, 13, 21758-21777. doi:10.18632/aging.203520. https://pubmed.ncbi.nlm.nih.gov/34520390/