智鼠故事 
C57BL/6JCya-Fgl2em1/Cya 基因敲除小鼠
复苏/繁育服务
产品名称：
Fgl2-KO
产品编号：
S-KO-02055
品系背景：
C57BL/6JCya
* 使用本品系发表的文献需注明：Fgl2-KO mice (Strain S-KO-02055) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
只
编辑策略
品系名称
C57BL/6JCya-Fgl2em1/Cya
品系编号
KOCMP-14190-Fgl2-B6J-VA
产品编号
S-KO-02055
基因名
Fgl2
品系背景
C57BL/6JCya
基因别称
musfiblp
NCBI ID
修饰方式
全身性基因敲除
NCBI RefSeq
NM_008013
Ensembl ID
ENSMUST00000035799
靶向范围
Exon 2
敲除长度
~1.1 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成，建议您在购买前查询最新的数据库和相关文献，以获取最准确的表型信息。
小鼠表型
MGI:103266 Homozygous null mice for one allele have unaltered type 1 immunity responses. Homozygous null mice for another allele show partial embryonic lethality, hemorrhage at implantation sites, decreased susceptibility to hepatitis virus infection and prolongedsurvival of heart grafts.
基因研究概述
Fgl2，即Fibrinogen-like protein 2，是一种在多种细胞类型中表达的基因，其编码的蛋白质在炎症、凝血、细胞凋亡、血管生成和免疫反应等生物学过程中发挥着重要作用。Fgl2基因的转录受多种转录因子的调控，包括Ets-1、Oct-1、Sp1和Sp3等。这些转录因子在Fgl2基因的启动子区域形成复杂的正调控域，与DNA结合，从而控制Fgl2基因的转录水平[3]。
Fgl2基因在不同类型的疾病中发挥着重要作用。在非酒精性脂肪性肝病（NAFLD）的病理过程中，Fgl2的表达水平显著升高，并与巨噬细胞的聚集和炎症反应密切相关。Fgl2基因敲除小鼠的肝脏脂质代谢紊乱和炎症反应得到明显改善，提示Fgl2基因在NAFLD的发生发展中具有重要作用[1]。此外，Fgl2基因的表达还与膀胱癌、急性髓细胞性白血病、胃癌、胶质瘤和慢性肾脏病等多种疾病的预后相关[2,4,5,6,8]。
Fgl2基因的转录水平在肿瘤相关巨噬细胞（TAMs）中发生变化，并与肿瘤的免疫微环境和预后相关。在非小细胞肺癌（NSCLC）中，Fgl2基因的表达与肺腺癌患者的预后相关，高表达Fgl2基因的患者预后较好[8]。在胃癌中，Fgl2基因的表达与患者的生存率相关，高表达Fgl2基因的患者预后较差[4]。在胶质瘤中，Fgl2基因的表达与患者的预后相关，高表达Fgl2基因的患者预后较差[6]。
Fgl2基因的表达还与慢性肾脏病（CKD）的进展和预后相关。Fgl2基因的表达在CKD患者中上调，并与肾纤维化和肾功能下降相关[9]。Fgl2基因的表达还与糖尿病心肌病的发生发展相关。在糖尿病心肌病模型中，Fgl2基因的沉默可以改善心肌细胞的功能，提示Fgl2基因在糖尿病心肌病的病理过程中具有重要作用[7]。
综上所述，Fgl2基因在多种疾病中发挥着重要作用，其表达水平的变化与疾病的预后相关。Fgl2基因的转录调控机制和功能研究有助于深入理解疾病的发病机制，为疾病的治疗和预防提供新的思路和策略。
参考文献：
1. Hu, Junjian, Wang, Hongwu, Li, Xitang, Ning, Qin, Wang, Xiaojing. 2020. Fibrinogen-like protein 2 aggravates nonalcoholic steatohepatitis via interaction with TLR4, eliciting inflammation in macrophages and inducing hepatic lipid metabolism disorder. In Theranostics, 10, 9702-9720. doi:10.7150/thno.44297. https://pubmed.ncbi.nlm.nih.gov/32863955/
2. Huang, Zhengnan, Wang, Zeyi, Xu, Chengdang, Zhang, Fang, Shen, Bing. 2024. FGL2 as a predictive biomarker for prognosis and immunotherapy in bladder cancer. In International journal of medical sciences, 21, 1447-1460. doi:10.7150/ijms.91874. https://pubmed.ncbi.nlm.nih.gov/38903931/
3. Liu, Mingfeng, Leibowitz, Julian L, Clark, David A, Marsden, Philip A, Levy, Gary A. . Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3. In European journal of biochemistry, 270, 2274-86. doi:. https://pubmed.ncbi.nlm.nih.gov/12752447/
4. Sullivan, Kevin M, Li, Haiqing, Yang, Annie, Fong, Yuman, Woo, Yanghee. 2024. Tumor and Peritoneum-Associated Macrophage Gene Signature as a Novel Molecular Biomarker in Gastric Cancer. In International journal of molecular sciences, 25, . doi:10.3390/ijms25074117. https://pubmed.ncbi.nlm.nih.gov/38612926/
5. Han, Haijun, Liu, Jie, Zhu, Shengyu, Zhao, Tiejun. . Identification of two key biomarkers CD93 and FGL2 associated with survival of acute myeloid leukaemia by weighted gene co-expression network analysis. In Journal of cellular and molecular medicine, 28, e18552. doi:10.1111/jcmm.18552. https://pubmed.ncbi.nlm.nih.gov/39054581/
6. Cao, Ziwen, Liu, Xing, Yan, Jun. 2024. Prognostic significance and gene co-expression network of CD16A and FGL2 in gliomas. In Frontiers in oncology, 14, 1447113. doi:10.3389/fonc.2024.1447113. https://pubmed.ncbi.nlm.nih.gov/39629005/
7. Zheng, Zhenzhong, Zhang, Fan, Gao, Dengpeng, Wu, Yujing, Wu, Hao. 2018. Gene expression profiles of rat MMECs with different glucose levels and fgl2 gene silencing. In Diabetes/metabolism research and reviews, 34, e3058. doi:10.1002/dmrr.3058. https://pubmed.ncbi.nlm.nih.gov/30098304/
8. Li, Zhaoxun, Zhou, Bin, Zhu, Xinsheng, Song, Xiao, Jiang, Gening. 2023. Differentiation-related genes in tumor-associated macrophages as potential prognostic biomarkers in non-small cell lung cancer. In Frontiers in immunology, 14, 1123840. doi:10.3389/fimmu.2023.1123840. https://pubmed.ncbi.nlm.nih.gov/36969247/
9. Denicolò, Sara, Nair, Viji, Leierer, Johannes, Ju, Wenjun, Perco, Paul. 2022. Assessment of Fibrinogen-like 2 (FGL2) in Human Chronic Kidney Disease through Transcriptomics Data Analysis. In Biomolecules, 13, . doi:10.3390/biom13010089. https://pubmed.ncbi.nlm.nih.gov/36671474/
质控标准
精子检测
① 冷冻前验证精子活力观察
② 冷冻验证每批次进行复苏验证
品系状态
活体
环境标准
SPF
供应地区
中国
