Slc29a1-KO 基因敲除小鼠

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产品名称

Slc29a1-KO 基因敲除小鼠

产品编号

S-KO-20407

品系全称

C57BL/6JCya-Slc29a1em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-63959-Slc29a1-B6J-VB

品系状态

使用本品系发表的文献需注明: Slc29a1-KO 基因敲除小鼠 mice (Strain S-KO-20407) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
solute carrier family 29 (nucleoside transporters), member 1
基因别称
1200014D21Rik,ENT1,mENT1
染色体号
Chr 17 (Mouse)
转录本 ID
NCBI: NM_001199113 | Ensembl: ENSMUST00000167692
修饰方式
全身性基因敲除
靶向范围
Exon 4~9
敲除长度
~1.9 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1927073Mice homozygous for a targeted null allele exhibit a slightly decreased body weight, increased alcohol preference and alcohol consumption, and reduced hypnotic and ataxic responses to ethanol associated with a reduction in adenosine tone. Adenosine uptake is almost completely abolished in mice homozygous for a knock-out allele.
SLC29A1,也称为人类平衡核苷转运蛋白1(hENT1),是一种位于细胞膜上的多跨膜蛋白,负责介导核苷、核碱基和某些治疗性类似物的转运。SLC29A1在多种生物学过程中发挥重要作用,包括细胞能量代谢、信号转导和药物转运等。SLC29A1的表达和活性受到多种因素的调控,如基因表达、转录后修饰和细胞内信号通路等。

SLC29A1在癌症的发生和发展中也发挥着重要作用。研究发现,SLC29A1的表达水平与多种癌症的预后相关。例如,在肝细胞癌(HCC)患者中,SLC29A1的低表达与较差的预后和肿瘤复发风险增加相关。SLC29A1的低表达可能通过促进上皮-间质转化(EMT)和激活核因子κB(NF-κB)信号通路等机制,促进肿瘤的侵袭和转移。此外,SLC29A1的表达还受到某些信号通路的调控,如整合素α3β1信号通路和STAT3信号通路等。

SLC29A1的基因多态性与某些药物的疗效和毒性相关。例如,SLC29A1的rs760370位点与慢性丙型肝炎(HCV)患者接受三联疗法(聚乙二醇干扰素α、利巴韦林和替拉普瑞韦)时利巴韦林引起的贫血严重程度相关。SLC29A1的rs760370 GG基因型与血红蛋白下降程度更严重相关,可能反映了红细胞对利巴韦林的摄取情况。此外,SLC29A1的基因多态性还可能影响氟嘧啶类药物在胃肠道癌症患者中的疗效和毒性。

综上所述,SLC29A1是一种重要的核苷转运蛋白,在多种生物学过程中发挥重要作用。SLC29A1的表达和活性受到多种因素的调控,并与其他信号通路相互作用,影响肿瘤的发生和发展。SLC29A1的基因多态性还可能影响某些药物的疗效和毒性,为个体化治疗提供新的思路和策略。未来,进一步研究SLC29A1的功能和调控机制,以及其在癌症治疗中的应用,将有助于提高癌症治疗的疗效和安全性[1,2,3,4,5,6,7,8]。

参考文献:
1. Koikawa, Kazuhiro, Kibe, Shin, Suizu, Futoshi, Zhou, Xiao Zhen, Lu, Kun Ping. 2021. Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy. In Cell, 184, 4753-4771.e27. doi:10.1016/j.cell.2021.07.020. https://pubmed.ncbi.nlm.nih.gov/34388391/
2. Sciarrillo, Rocco, Wojtuszkiewicz, Anna, Assaraf, Yehuda G, Giovannetti, Elisa, Cloos, Jacqueline. 2020. The role of alternative splicing in cancer: From oncogenesis to drug resistance. In Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy, 53, 100728. doi:10.1016/j.drup.2020.100728. https://pubmed.ncbi.nlm.nih.gov/33070093/
3. Farías, Marcelo, San Martín, Rody, Puebla, Carlos, Casanello, Paola, Sobrevia, Luis. . Nitric oxide reduces adenosine transporter ENT1 gene (SLC29A1) promoter activity in human fetal endothelium from gestational diabetes. In Journal of cellular physiology, 208, 451-60. doi:. https://pubmed.ncbi.nlm.nih.gov/16688763/
4. Gao, Ping-Ting, Cheng, Jian-Wen, Gong, Zi-Jun, Fan, Jia, Yang, Xin-Rong. 2017. Low SLC29A1 expression is associated with poor prognosis in patients with hepatocellular carcinoma. In American journal of cancer research, 7, 2465-2477. doi:. https://pubmed.ncbi.nlm.nih.gov/29312800/
5. Liu, Mingyang, Zhang, Yuqing, Yang, Jingxuan, Houchen, Courtney W, Li, Min. 2019. ZIP4 Increases Expression of Transcription Factor ZEB1 to Promote Integrin α3β1 Signaling and Inhibit Expression of the Gemcitabine Transporter ENT1 in Pancreatic Cancer Cells. In Gastroenterology, 158, 679-692.e1. doi:10.1053/j.gastro.2019.10.038. https://pubmed.ncbi.nlm.nih.gov/31711924/
6. Boswell-Casteel, Rebba C, Hays, Franklin A. 2016. Equilibrative nucleoside transporters-A review. In Nucleosides, nucleotides & nucleic acids, 36, 7-30. doi:10.1080/15257770.2016.1210805. https://pubmed.ncbi.nlm.nih.gov/27759477/
7. Fernandes, Marianne Rodrigues, Rodrigues, Juliana Carla Gomes, Dobbin, Elizabeth Ayres Fragoso, de Assumpção, Paulo Pimentel, Dos Santos, Ney Pereira Carneiro. 2021. Influence of FPGS, ABCC4, SLC29A1, and MTHFR genes on the pharmacogenomics of fluoropyrimidines in patients with gastrointestinal cancer from the Brazilian Amazon. In Cancer chemotherapy and pharmacology, 88, 837-844. doi:10.1007/s00280-021-04327-w. https://pubmed.ncbi.nlm.nih.gov/34331561/
8. Milazzo, Laura, Peri, Anna Maria, Mazzali, Cristina, Antinori, Spinello, Falvella, Felicia Stefania. 2015. SLC29A1 polymorphism and prediction of anaemia severity in patients with chronic hepatitis C receiving triple therapy with telaprevir. In The Journal of antimicrobial chemotherapy, 70, 1155-60. doi:10.1093/jac/dku519. https://pubmed.ncbi.nlm.nih.gov/25583751/