Il10rb-KO 基因敲除小鼠

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产品名称

Il10rb-KO 基因敲除小鼠

产品编号

S-KO-19806

品系全称

C57BL/6JCya-Il10rbem1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-16155-Il10rb-B6J-VB

品系状态

使用本品系发表的文献需注明: Il10rb-KO 基因敲除小鼠 mice (Strain S-KO-19806) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
KO小鼠库模型
JAK-STAT信号通路

基本信息

基因研究概述

质控标准

基因
基因全称
interleukin 10 receptor, beta
基因别称
6620401D04Rik,CRF2-4,Crfb4,D16H21S58,D21S58h,IL-10R2,Il10r2
染色体号
Chr 16 (Mouse)
转录本 ID
NCBI: NM_008349 | Ensembl: ENSMUST00000023691
修饰方式
全身性基因敲除
靶向范围
Exon 3~4
敲除长度
~3.7 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:109380Most mice homozygous for a knock-out allele develop moderate to severe colitis without small intestinal involvement and splenomegaly with a hyperproliferative splenic red pulp.
IL10RB基因编码的是白介素-10受体β链(IL-10Rβ),这是一种细胞表面受体蛋白,属于白介素受体家族。IL-10Rβ与白介素-10受体α链(IL-10Rα)结合形成异源二聚体,共同作为白介素-10(IL-10)的受体。IL-10是一种具有多种生物活性的细胞因子,主要功能包括抑制免疫细胞如巨噬细胞、T细胞和B细胞的活性,以及调节炎症反应和免疫应答。

IL-10RB在多种生理和病理过程中发挥重要作用。在肠道生理学中,IL-10RB对于潘氏细胞的形成至关重要。潘氏细胞是肠道上皮细胞的一种,负责产生抗菌肽,对抗肠道病原体。研究表明,IL-10Rβ在潘氏细胞的发育和功能维持中起着关键作用[1]。

在COVID-19疾病中,IL-10RB基因也被发现与疾病易感性和严重程度相关。研究显示,IL-10RB基因的遗传变异与COVID-19的严重程度有关,其表达上调与疾病的不良预后相关[2,4,5,6]。此外,IL-10RB的过表达还与病毒负载增加和免疫相关信号通路的激活有关[2,5,6]。

除了在肠道生理和COVID-19疾病中的作用外,IL-10RB还与其他疾病有关,例如炎症性肠病和系统性红斑狼疮。在炎症性肠病中,IL-10RB基因的突变可能导致婴儿期发病的炎症性肠病[3,9]。在系统性红斑狼疮中,IL-10RB基因的某些多态性与疾病易感性和临床表现相关[8]。

此外,IL-10RB还与肥胖相关。研究表明,IL-10RB基因的某些多态性与肥胖的参数相关,例如体脂百分比[7]。

综上所述,IL10RB基因编码的白介素-10受体β链在多种生理和病理过程中发挥重要作用,包括肠道生理、COVID-19疾病、炎症性肠病、系统性红斑狼疮和肥胖。IL10RB基因的遗传变异和表达水平与疾病的易感性和严重程度相关,为其作为疾病治疗和预防的潜在靶点提供了理论基础。

参考文献:
1. He, Gui-Wei, Lin, Lin, DeMartino, Jeff, Holstege, Frank, Clevers, Hans. 2022. Optimized human intestinal organoid model reveals interleukin-22-dependency of paneth cell formation. In Cell stem cell, 29, 1333-1345.e6. doi:10.1016/j.stem.2022.08.002. https://pubmed.ncbi.nlm.nih.gov/36002022/
2. Voloudakis, Georgios, Vicari, James M, Venkatesh, Sanan, Fullard, John F, Roussos, Panos. 2022. A translational genomics approach identifies IL10RB as the top candidate gene target for COVID-19 susceptibility. In NPJ genomic medicine, 7, 52. doi:10.1038/s41525-022-00324-x. https://pubmed.ncbi.nlm.nih.gov/36064543/
3. Motallebi, Farzaneh, Al Sudani, Zainab M, Vaghefi, Fatemeh, Kowsari, Ali, Oladnabi, Morteza. 2024. A novel biallelic 19-bp deletion in the IL10RB gene caused infant-onset inflammatory bowel disease in a consanguineous family: a molecular docking simulation study and literature review. In Molecular biology reports, 51, 223. doi:10.1007/s11033-024-09248-4. https://pubmed.ncbi.nlm.nih.gov/38281300/
4. Kousathanas, Athanasios, Pairo-Castineira, Erola, Rawlik, Konrad, Caulfield, Mark J, Baillie, J Kenneth. 2022. Whole-genome sequencing reveals host factors underlying critical COVID-19. In Nature, 607, 97-103. doi:10.1038/s41586-022-04576-6. https://pubmed.ncbi.nlm.nih.gov/35255492/
5. Krishnamoorthy, Suhas, Li, Gloria H-Y, Cheung, Ching-Lung. 2022. Transcriptome-wide summary data-based Mendelian randomization analysis reveals 38 novel genes associated with severe COVID-19. In Journal of medical virology, 95, e28162. doi:10.1002/jmv.28162. https://pubmed.ncbi.nlm.nih.gov/36127160/
6. Voloudakis, Georgios, Hoffman, Gabriel, Venkatesh, Sanan, Fullard, John F, Roussos, Panos. 2021. IL10RB as a key regulator of COVID-19 host susceptibility and severity. In medRxiv : the preprint server for health sciences, , . doi:10.1101/2021.05.31.21254851. https://pubmed.ncbi.nlm.nih.gov/34100031/
7. Maculewicz, Ewelina, Dzitkowska-Zabielska, Magdalena, Antkowiak, Bożena, Dziuda, Łukasz, Cięszczyk, Paweł. 2022. Association of Interleukin Genes IL10 and IL10RB with Parameters of Overweight in Military Students. In Genes, 13, . doi:10.3390/genes13020291. https://pubmed.ncbi.nlm.nih.gov/35205336/
8. Grk, Milka, Miskovic, Rada, Jeremic, Ivica, Cirkovic, Andja, Banko, Ana. 2023. Association of IL10RA, IL10RB, and IL22RA Polymorphisms/Haplotypes with Susceptibility to and Clinical Manifestations of SLE. In International journal of molecular sciences, 24, . doi:10.3390/ijms241411292. https://pubmed.ncbi.nlm.nih.gov/37511050/
9. Yazdani, Reza, Moazzami, Bobak, Madani, Seyedeh Panid, Abolhassani, Hassan, Aghamohammadi, Asghar. 2019. Candidiasis associated with very early onset inflammatory bowel disease: First IL10RB deficient case from the National Iranian Registry and review of the literature. In Clinical immunology (Orlando, Fla.), 205, 35-42. doi:10.1016/j.clim.2019.05.007. https://pubmed.ncbi.nlm.nih.gov/31096038/