Reg2-KO 基因敲除小鼠

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产品名称

Reg2-KO 基因敲除小鼠

产品编号

S-KO-19503

品系全称

C57BL/6JCya-Reg2em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-19693-Reg2-B6J-VC

品系状态

使用本品系发表的文献需注明: Reg2-KO 基因敲除小鼠 mice (Strain S-KO-19503) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
regenerating islet-derived 2
基因别称
PSP,PTP,REG-2
染色体号
Chr 6 (Mouse)
转录本 ID
NCBI: NM_009043 | Ensembl: ENSMUST00000023906
修饰方式
全身性基因敲除
靶向范围
Exon 3~6
敲除长度
~3.4 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:97896Mice homozygous for a null allele exhibit impaired suckling, delayed hypoglossal myelination and reduced liver regeneration following partial hepatectomy.
基因Reg2,也称为Regenerating islet-derived protein 2(Reg II),是一种在哺乳动物中表达的蛋白质。在胰腺中,Reg2主要在胰岛β细胞中表达,并且在糖尿病、胰腺炎和胰腺再生的过程中显著增加[3]。Reg2的功能与细胞生长、存活和/或再生有关[2]。

在酵母菌Saccharomyces cerevisiae中,Reg2基因编码一个与类型1蛋白磷酸酶(PP1)结合的蛋白。Reg2蛋白与Glc7p相互作用,Glc7p是类型1蛋白磷酸酶的催化亚基,对于细胞生长至关重要[1]。Reg2与Glc7p和Reg1p共同作用,与Snf1蛋白激酶一起调节生长。Reg2突变体在单突变中表现出野生型表型,但Reg1和Reg2的双突变体表现出严重的生长缺陷[1]。

Reg2的表达和功能与氧化应激和抗氧化酶有关。在胰腺胰岛细胞中,Reg2的表达受到谷胱甘肽过氧化物酶-1(GPX1)的调节。GPX1过表达导致Reg2表达下调,而GPX1敲除或过表达GPX1的模拟物则导致Reg2表达上调[5]。此外,Reg2的表达和功能也与超氧化物歧化酶-1(Sod1)有关[4]。

Reg2的表达和功能受到多种因素的调节。在胰腺胰岛细胞中,糖皮质激素和白细胞介素-6可以转录激活Reg2和Reg3β基因[6]。此外,IFNβ也可以上调Reg1和Reg2基因的表达[7]。

综上所述,基因Reg2在细胞生长、存活和再生中发挥重要作用。Reg2的表达和功能与氧化应激、抗氧化酶和多种因素有关。Reg2的研究有助于深入理解细胞生长、存活和再生的机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Frederick, D L, Tatchell, K. . The REG2 gene of Saccharomyces cerevisiae encodes a type 1 protein phosphatase-binding protein that functions with Reg1p and the Snf1 protein kinase to regulate growth. In Molecular and cellular biology, 16, 2922-31. doi:. https://pubmed.ncbi.nlm.nih.gov/8649403/
2. Li, Bing, Wang, Xiao, Liu, Jun-Li. 2010. Pancreatic acinar-specific overexpression of Reg2 gene offered no protection against either experimental diabetes or pancreatitis in mice. In American journal of physiology. Gastrointestinal and liver physiology, 299, G413-21. doi:10.1152/ajpgi.00500.2009. https://pubmed.ncbi.nlm.nih.gov/20489047/
3. Li, Qing, Li, Bing, Miao, Xiaoliang, Gao, Zu-Hua, Liu, Jun-Li. . Reg2 Expression Is Required for Pancreatic Islet Compensation in Response to Aging and High-Fat Diet-Induced Obesity. In Endocrinology, 158, 1634-1644. doi:10.1210/en.2016-1551. https://pubmed.ncbi.nlm.nih.gov/28009527/
4. Wang, Hong, Vatamaniuk, Marko Z, Zhao, Zeping, Lei, Xin Gen. 2023. Interdependencies of Gene Expression and Function between Two Redox Enzymes and REG Family Proteins in Murine Pancreatic Islets and Human Pancreatic Cells. In Antioxidants (Basel, Switzerland), 12, . doi:10.3390/antiox12040849. https://pubmed.ncbi.nlm.nih.gov/37107224/
5. Yun, Jun-Won, Zhao, Zeping, Yan, Xi, Vatamaniuk, Marko Z, Lei, Xin Gen. 2019. Glutathione peroxidase-1 inhibits transcription of regenerating islet-derived protein-2 in pancreatic islets. In Free radical biology & medicine, 134, 385-393. doi:10.1016/j.freeradbiomed.2019.01.024. https://pubmed.ncbi.nlm.nih.gov/30703484/
6. Luo, Chen, Li, Bing, Liu, Lu, Wang, Min, Liu, Jun-Li. 2012. Transcriptional activation of Reg2 and Reg3β genes by glucocorticoids and interleukin-6 in pancreatic acinar and islet cells. In Molecular and cellular endocrinology, 365, 187-96. doi:10.1016/j.mce.2012.10.018. https://pubmed.ncbi.nlm.nih.gov/23147030/
7. Planas, R, Alba, A, Carrillo, J, Verdaguer, J, Vives-Pi, M. 2006. Reg (regenerating) gene overexpression in islets from non-obese diabetic mice with accelerated diabetes: role of IFNbeta. In Diabetologia, 49, 2379-87. doi:. https://pubmed.ncbi.nlm.nih.gov/16900387/