Shisa7-KO 基因敲除小鼠

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产品名称

Shisa7-KO 基因敲除小鼠

产品编号

S-KO-19402

品系全称

C57BL/6JCya-Shisa7em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-232813-Shisa7-B6J-VC

品系状态

使用本品系发表的文献需注明: Shisa7-KO 基因敲除小鼠 mice (Strain S-KO-19402) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
shisa family member 7
基因别称
ckamp59
染色体号
Chr 7 (Mouse)
转录本 ID
NCBI: NM_172737 | Ensembl: ENSMUST00000066041
修饰方式
全身性基因敲除
靶向范围
Exon 3
敲除长度
~1.7 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:3605641Homozygous knockout results in impaired expression of contextual fear memory.
Shisa7(CKAMP59)是一种重要的AMPA受体(AMPAR)辅助蛋白,参与调节突触传递和可塑性。AMPA受体在突触传递和可塑性中发挥着至关重要的作用,而Shisa7与AMPA受体相互作用,影响其通道动力学、受体转运和亚细胞定位。Shisa7基因的缺失会导致AMPAR电流加快,影响突触传递和可塑性。在Shisa7基因敲除小鼠中,长时程增强(LTP)的启动和维持受到抑制,这表明Shisa7在突触可塑性中发挥着重要作用。此外,Shisa7基因敲除小鼠在条件性恐惧记忆方面存在缺陷,这表明Shisa7在记忆形成和回忆中发挥着重要作用。

在脊椎动物中,Shisa7与AMPA受体相互作用,影响其通道动力学、受体转运和亚细胞定位。在无脊椎动物中,Shisa7基因的同源基因被发现,并证实其在GABAAR中的作用。例如,在二斑叶螨中,Shisa基因的同源基因被证实可以降低GABAAR对GABA的敏感性。这表明Shisa7在脊椎动物和无脊椎动物中发挥着重要作用,参与调节神经递质受体的功能和突触传递。

此外,研究发现,Shisa7的表达受到miRNA的调控。例如,hsa-miR-3681可以与Shisa7的3'非编码区(3'UTR)结合,抑制Shisa7的表达。这表明miRNA在调节Shisa7表达和功能中发挥着重要作用。

Shisa7在多种疾病中发挥重要作用,包括阿尔茨海默病(AD)和青光眼。在AD中,研究发现BCAS4/hsa-miR-185-5p/SHISA7 ceRNA轴在AD的tau病理发生中发挥重要作用。在青光眼中,研究发现Shisa7的表达与青光眼的发生和进展相关。

综上所述,Shisa7是一种重要的AMPAR辅助蛋白,参与调节突触传递和可塑性。Shisa7在脊椎动物和无脊椎动物中发挥着重要作用,参与调节神经递质受体的功能和突触传递。Shisa7的表达受到miRNA的调控。Shisa7在多种疾病中发挥重要作用,包括AD和青光眼。Shisa7的研究有助于深入理解神经递质受体的功能和突触传递的调节机制,为疾病的治疗和预防提供新的思路和策略[1,2,3,4,5,6,7,8,9,10]。

参考文献:
1. Schmitz, Leanne J M, Klaassen, Remco V, Ruiperez-Alonso, Marta, Smit, August B, Spijker, Sabine. 2017. The AMPA receptor-associated protein Shisa7 regulates hippocampal synaptic function and contextual memory. In eLife, 6, . doi:10.7554/eLife.24192. https://pubmed.ncbi.nlm.nih.gov/29199957/
2. von Engelhardt, Jakob. 2019. AMPA Receptor Auxiliary Proteins of the CKAMP Family. In International journal of molecular sciences, 20, . doi:10.3390/ijms20061460. https://pubmed.ncbi.nlm.nih.gov/30909450/
3. Lee, Hee-Eun, Park, Sang-Je, Huh, Jae-Won, Imai, Hiroo, Kim, Heui-Soo. . Enhancer Function of MicroRNA-3681 Derived from Long Terminal Repeats Represses the Activity of Variable Number Tandem Repeats in the 3' UTR of SHISA7. In Molecules and cells, 43, 607-618. doi:10.14348/molcells.2020.0058. https://pubmed.ncbi.nlm.nih.gov/32655015/
4. Sabaie, Hani, Talebi, Mahnaz, Gharesouarn, Jalal, Jalili Khoshnoud, Reza, Rezazadeh, Maryam. 2022. Identification and Analysis of BCAS4/hsa-miR-185-5p/SHISA7 Competing Endogenous RNA Axis in Late-Onset Alzheimer's Disease Using Bioinformatic and Experimental Approaches. In Frontiers in aging neuroscience, 14, 812169. doi:10.3389/fnagi.2022.812169. https://pubmed.ncbi.nlm.nih.gov/35264942/
5. Upreti, Anil, Hoang, Thanh V, Li, Minghua, Lachke, Salil A, Robinson, Michael L. . miR-26 Deficiency Causes Alterations in Lens Transcriptome and Results in Adult-Onset Cataract. In Investigative ophthalmology & visual science, 65, 42. doi:10.1167/iovs.65.4.42. https://pubmed.ncbi.nlm.nih.gov/38683565/
6. Ellis, Randall J, Ferland, Jacqueline-Marie N, Rahman, Tanni, Dracheva, Stella, Hurd, Yasmin L. 2024. Machine learning analysis of the orbitofrontal cortex transcriptome of human opioid users identifies Shisa7 as a translational target relevant for heroin-seeking leveraging a male rat model. In Biological psychiatry, , . doi:10.1016/j.biopsych.2024.12.007. https://pubmed.ncbi.nlm.nih.gov/39725299/
7. Zhan, Enling, Jiang, Jie, Wang, Ying, Wang, Qiuxia, Zhao, Chunqing. 2023. Shisa reduces the sensitivity of homomeric RDL channel to GABA in the two-spotted spider mite, Tetranychus urticae Koch. In Pesticide biochemistry and physiology, 192, 105414. doi:10.1016/j.pestbp.2023.105414. https://pubmed.ncbi.nlm.nih.gov/37105623/
8. Upreti, Anil, Hoang, Thanh V, Li, Minghua, Lachke, Salil A, Robinson, Michael L. 2024. miR-26 deficiency causes alterations in lens transcriptome and results in adult-onset cataract. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.01.29.577818. https://pubmed.ncbi.nlm.nih.gov/38352453/
9. Wang, Xiaoqin, Chen, Ming, Liu, Longqian, Zeng, Liuzhi. . Integrated aqueous humor ceRNA and miRNA-TF-mRNA network analysis reveals potential molecular mechanisms governing primary open-angle glaucoma pathogenesis. In Indian journal of ophthalmology, 71, 553-559. doi:10.4103/ijo.IJO_1448_22. https://pubmed.ncbi.nlm.nih.gov/36727359/
10. Zhao, Yangxing, Xue, Feng, Sun, Jinfeng, Yu, Jian, Xia, Qiang. 2014. Genome-wide methylation profiling of the different stages of hepatitis B virus-related hepatocellular carcinoma development in plasma cell-free DNA reveals potential biomarkers for early detection and high-risk monitoring of hepatocellular carcinoma. In Clinical epigenetics, 6, 30. doi:10.1186/1868-7083-6-30. https://pubmed.ncbi.nlm.nih.gov/25859288/