Cald1-KO 基因敲除小鼠

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产品名称

Cald1-KO 基因敲除小鼠

产品编号

S-KO-19001

品系全称

C57BL/6JCya-Cald1em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-109624-Cald1-B6J-VA

品系状态

使用本品系发表的文献需注明: Cald1-KO 基因敲除小鼠 mice (Strain S-KO-19001) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
caldesmon 1
基因别称
4833423D12Rik,C920027I18Rik,caldesmon
染色体号
Chr 6 (Mouse)
转录本 ID
NCBI: NM_001347100 | Ensembl: ENSMUST00000115026
修饰方式
全身性基因敲除
靶向范围
Exon 6
敲除长度
~0.1 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:88250Some homozygous mutant mice develop hernia and those that do, die within 5-7 hours after birth. Mice homozygous for a different targeted allele fail to develop. Mice heterozygous for this allele exhibit increased urinary bladder weight, smooth muscle bundles and non-voiding contractions.
CALD1(Caldesmon 1)是一种细胞骨架蛋白,主要存在于平滑肌细胞和成纤维细胞中,参与调节细胞形态、细胞骨架重组和细胞运动等细胞过程。CALD1的表达和功能与多种疾病的发生和发展密切相关,包括肺部疾病、癌症、糖尿病视网膜病变等。

在肺部疾病中,CALD1的表达水平与肺纤维化的发生和发展密切相关。研究表明,CALD1在肺纤维化模型中表达上调,其敲低可以抑制肺纤维化相关基因的表达,提示CALD1可能参与肺纤维化的发生机制[1]。此外,CALD1在膀胱癌中也表现出重要的生物学功能。研究发现,CALD1在膀胱癌组织中表达上调,并且与膀胱癌的进展和预后相关。CALD1的表达水平与膀胱癌的分级、分期、淋巴结转移等相关,其高表达提示预后不良[2,3]。此外,CALD1还与膀胱癌细胞的上皮-间质转化(EMT)过程相关,通过调节PI3K-Akt信号通路促进EMT的发生[8]。

CALD1在糖尿病视网膜病变中也发挥重要作用。研究发现,CALD1在糖尿病视网膜病变的纤维血管膜中表达上调,并且与M2型巨噬细胞的浸润相关。CALD1的高表达提示糖尿病视网膜病变的进展和预后不良[5]。

除了上述疾病,CALD1还与胃癌、卵巢癌、结直肠癌等癌症的发生和发展相关。研究发现,CALD1在胃癌、卵巢癌、结直肠癌组织中表达上调,并且与癌症的进展和预后相关。CALD1的高表达提示预后不良,并且与免疫浸润相关[4,6,7]。此外,CALD1还通过JAK/STAT信号通路促进PD-L1的表达,从而促进膀胱癌的进展[9]。

综上所述,CALD1是一种重要的细胞骨架蛋白,参与调节细胞形态、细胞骨架重组和细胞运动等细胞过程。CALD1的表达和功能与多种疾病的发生和发展密切相关,包括肺部疾病、癌症、糖尿病视网膜病变等。CALD1的研究有助于深入理解细胞骨架蛋白在疾病发生和发展中的作用机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Wu, Shufei, Liu, Mengying, Zhang, Mingrui, Huang, Xinmei, Cao, Mengshu. 2024. The gene expression of CALD1, CDH2, and POSTN in fibroblast are related to idiopathic pulmonary fibrosis. In Frontiers in immunology, 15, 1275064. doi:10.3389/fimmu.2024.1275064. https://pubmed.ncbi.nlm.nih.gov/38370408/
2. Du, YiHeng, Jiang, Xiang, Wang, Bo, Wang, XiZhi, Liu, HaiTao. 2021. The cancer-associated fibroblasts related gene CALD1 is a prognostic biomarker and correlated with immune infiltration in bladder cancer. In Cancer cell international, 21, 283. doi:10.1186/s12935-021-01896-x. https://pubmed.ncbi.nlm.nih.gov/34051818/
3. Zhang, Sheng, Wang, Qin, Li, Wenfeng, Chen, Jinzhong. 2022. MIR100HG Regulates CALD1 Gene Expression by Targeting miR-142-5p to Affect the Progression of Bladder Cancer Cells in vitro, as Revealed by Transcriptome Sequencing. In Frontiers in molecular biosciences, 8, 793493. doi:10.3389/fmolb.2021.793493. https://pubmed.ncbi.nlm.nih.gov/35127818/
4. Zheng, Hang, Bai, Yuge, Wang, Jingui, Liu, Yucun, Wang, Xin. 2021. Weighted Gene Co-expression Network Analysis Identifies CALD1 as a Biomarker Related to M2 Macrophages Infiltration in Stage III and IV Mismatch Repair-Proficient Colorectal Carcinoma. In Frontiers in molecular biosciences, 8, 649363. doi:10.3389/fmolb.2021.649363. https://pubmed.ncbi.nlm.nih.gov/33996905/
5. Meng, Zhishang, Chen, Yanzhu, Wu, Wenyi, Yao, Xiaoxi, Luo, Jing. 2022. Exploring the Immune Infiltration Landscape and M2 Macrophage-Related Biomarkers of Proliferative Diabetic Retinopathy. In Frontiers in endocrinology, 13, 841813. doi:10.3389/fendo.2022.841813. https://pubmed.ncbi.nlm.nih.gov/35692390/
6. Liu, Yixuan, Xie, Suhong, Zhu, Keyu, Guo, Lin, Lu, Renquan. 2021. CALD1 is a prognostic biomarker and correlated with immune infiltrates in gastric cancers. In Heliyon, 7, e07257. doi:10.1016/j.heliyon.2021.e07257. https://pubmed.ncbi.nlm.nih.gov/34189308/
7. Li, Wei, Huang, Limei, Qi, Nana, Zhang, Qinle, Qin, Zailong. 2024. Upregulation of CALD1 predicted a poor prognosis for platinum-treated ovarian cancer and revealed it as a potential therapeutic resistance target. In BMC genomics, 25, 183. doi:10.1186/s12864-024-10056-0. https://pubmed.ncbi.nlm.nih.gov/38365611/
8. Ma, Wen-Qian, Miao, Ming-Chang, Ding, Ping-An, Zhang, Zhi-Dong, Zhao, Qun. . CALD1 facilitates epithelial-mesenchymal transition progression in gastric cancer cells by modulating the PI3K-Akt pathway. In World journal of gastrointestinal oncology, 16, 1029-1045. doi:10.4251/wjgo.v16.i3.1029. https://pubmed.ncbi.nlm.nih.gov/38577446/
9. Li, Cheng, Yang, Fuhan, Wang, Ruiliang, Wang, Hong, Yao, Xudong. . CALD1 promotes the expression of PD-L1 in bladder cancer via the JAK/STAT signaling pathway. In Annals of translational medicine, 9, 1441. doi:10.21037/atm-21-4192. https://pubmed.ncbi.nlm.nih.gov/34733993/