Mex3b-KO 基因敲除小鼠

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产品名称

Mex3b-KO 基因敲除小鼠

产品编号

S-KO-18492

品系全称

C57BL/6JCya-Mex3bem1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-108797-Mex3b-B6J-VA

品系状态

使用本品系发表的文献需注明: Mex3b-KO 基因敲除小鼠 mice (Strain S-KO-18492) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
mex3 RNA binding family member B
基因别称
4931439A04Rik,M-04814,Rkhd3,mKIAA2009
染色体号
Chr 7 (Mouse)
转录本 ID
NCBI: NM_175366 | Ensembl: ENSMUST00000082237
修饰方式
全身性基因敲除
靶向范围
Exon 2
敲除长度
~5.2 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1918252Homozygous inactivation of this gene leads to partial neonatal lethality, decreased body weight and subfertility. Males show seminiferous tubule obstruction, oligozoospermia, abnormalities in Sertoli cell barrier morphology and function, and impaired Sertoli cell and macrophage phagocytosis.
Mex3b是一种进化上保守的RNA结合蛋白,属于Mex3家族,在哺乳动物中存在四个相关的成员。Mex3b在多种生物学过程中发挥重要作用,包括细胞分化、发育、免疫反应和肿瘤发生等。Mex3b的主要功能是通过结合到mRNA的3'非翻译区(3'UTR)来调节其稳定性和翻译效率。Mex3b可以与多种mRNA相互作用,包括自身mRNA,形成一个负反馈调节环路,从而精细调控基因表达水平。

Mex3b在生殖系统中发挥重要作用。研究表明,Mex3b基因敲除小鼠的精子发生过程受损,表现为精子数量减少和精子活力下降[2]。Mex3b通过调节Rap1信号通路,影响支持细胞的吞噬功能和细胞间连接,进而影响血睾屏障的形成和完整性[2]。

Mex3b在炎症反应中也发挥重要作用。研究发现,Mex3b在慢性鼻窦炎伴鼻息肉患者的鼻上皮细胞中表达下调,而IL-37可以抑制Mex3b的表达,进而抑制Toll样受体3(TLR3)介导的炎症反应[1]。此外,Mex3b还可以与HOTAIR相互作用,促进Runx3的泛素化降解,从而增强胃癌细胞的侵袭能力[7]。

Mex3b在肿瘤发生中也发挥重要作用。研究发现,Mex3b在非小细胞肺癌(NSCLC)中表达上调,与患者的不良预后相关[8]。Mex3b可以与HOTAIR相互作用,促进HBV感染和肝细胞癌的发生[6]。此外,Mex3b还可以通过下调HLA-A的表达,介导肿瘤细胞对免疫治疗的抵抗[3]。

Mex3b在早期胚胎发育中也发挥重要作用。研究发现,Mex3b在非洲爪蟾早期胚胎发育过程中参与神经板的背腹轴模式形成,通过调节FGF信号通路,影响神经系统的发育[4]。

Mex3b在骨骼肌细胞中也发挥重要作用。研究发现,在低温条件下,Mex3b的表达下调,通过抑制Kras的表达,调节细胞凋亡相关基因和蛋白的表达,从而影响细胞存活[5]。

综上所述,Mex3b是一种重要的RNA结合蛋白,在多种生物学过程中发挥重要作用,包括生殖、炎症反应、肿瘤发生和早期胚胎发育等。Mex3b的研究有助于深入理解RNA调控的生物学功能和疾病发生机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Liu, Jin-Xin, Liao, Bo, Yu, Qi-Hong, Zhen, Guohua, Liu, Zheng. 2019. The IL-37-Mex3B-Toll-like receptor 3 axis in epithelial cells in patients with eosinophilic chronic rhinosinusitis with nasal polyps. In The Journal of allergy and clinical immunology, 145, 160-172. doi:10.1016/j.jaci.2019.07.009. https://pubmed.ncbi.nlm.nih.gov/31330219/
2. Le Borgne, Maïlys, Chartier, Nicolas, Buchet-Poyau, Karine, Perrard, Marie-Hélène, Billaud, Marc. . The RNA-binding protein Mex3b regulates the spatial organization of the Rap1 pathway. In Development (Cambridge, England), 141, 2096-107. doi:10.1242/dev.108514. https://pubmed.ncbi.nlm.nih.gov/24803656/
3. Huang, Lu, Malu, Shruti, McKenzie, Jodi A, Woodman, Scott E, Hwu, Patrick. 2018. The RNA-binding Protein MEX3B Mediates Resistance to Cancer Immunotherapy by Downregulating HLA-A Expression. In Clinical cancer research : an official journal of the American Association for Cancer Research, 24, 3366-3376. doi:10.1158/1078-0432.CCR-17-2483. https://pubmed.ncbi.nlm.nih.gov/29496759/
4. Takada, Hitomi, Kawana, Takahiro, Ito, Yuzuru, Asashima, Makoto, Taira, Masanori. . The RNA-binding protein Mex3b has a fine-tuning system for mRNA regulation in early Xenopus development. In Development (Cambridge, England), 136, 2413-22. doi:10.1242/dev.029165. https://pubmed.ncbi.nlm.nih.gov/19542354/
5. Umehara, Takahiro, Kagawa, Shinichiro, Tomida, Aiko, Shingu, Keita, Ikematsu, Kazuya. 2020. Body temperature-dependent microRNA expression analysis in rats: rno-miR-374-5p regulates apoptosis in skeletal muscle cells via Mex3B under hypothermia. In Scientific reports, 10, 15432. doi:10.1038/s41598-020-71931-w. https://pubmed.ncbi.nlm.nih.gov/32963265/
6. Zhang, Hao, Xing, Zheng, Mani, Saravana Kumar Kailasam, Merle, Philippe, Andrisani, Ourania. 2016. RNA helicase DEAD box protein 5 regulates Polycomb repressive complex 2/Hox transcript antisense intergenic RNA function in hepatitis B virus infection and hepatocarcinogenesis. In Hepatology (Baltimore, Md.), 64, 1033-48. doi:10.1002/hep.28698. https://pubmed.ncbi.nlm.nih.gov/27338022/
7. Xue, Meng, Chen, Lu-Yi, Wang, Wei-Jia, Wang, Liang-Jing, Chen, Shu-Jie. 2018. HOTAIR induces the ubiquitination of Runx3 by interacting with Mex3b and enhances the invasion of gastric cancer cells. In Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association, 21, 756-764. doi:10.1007/s10120-018-0801-6. https://pubmed.ncbi.nlm.nih.gov/29417297/
8. Zhang, Ming, Cao, Linfeng, Hou, Gouxin, Lv, Xiaodong, Deng, Jingjing. 2022. Investigation of the Potential Correlation Between RNA-Binding Proteins in the Evolutionarily Conserved MEX3 Family and Non-small-Cell Lung Cancer. In Molecular biotechnology, 65, 1263-1274. doi:10.1007/s12033-022-00638-2. https://pubmed.ncbi.nlm.nih.gov/36507941/