Themis2-KO 基因敲除小鼠

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产品名称

Themis2-KO 基因敲除小鼠

产品编号

S-KO-18296

品系全称

C57BL/6JCya-Themis2em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-230787-Themis2-B6J-VB

品系状态

使用本品系发表的文献需注明: Themis2-KO 基因敲除小鼠 mice (Strain S-KO-18296) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
thymocyte selection associated family member 2
基因别称
ICB-1
染色体号
Chr 4 (Mouse)
转录本 ID
NCBI: NM_001033308 | Ensembl: ENSMUST00000045154
修饰方式
全身性基因敲除
靶向范围
Exon 4
敲除长度
~2.3 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:2446213Mice homozygous for a knock-out allele exhibit normal B cell development, activation, and antibody responses.
Themis2基因,也被称为Thymocyte-expressed molecule involved in selection (Themis),是一种在免疫细胞中表达的蛋白质。Themis2与信号传导蛋白Grb2和Vav1相关联,并在B细胞受体(BCR)刺激后发生酪氨酸磷酸化。Themis2最初被认为可能在B细胞发育和激活中发挥重要作用,但研究表明Themis2并不是B细胞发育、激活或抗体反应所必需的[3]。

Themis2在癌症研究中受到了越来越多的关注。研究显示,Themis2在甲状腺癌、肝细胞癌、卵巢癌和三阴性乳腺癌(TNBC)等多种癌症中表达上调,并与预后不良相关。例如,Themis2的表达与甲状腺癌患者的免疫浸润水平呈正相关,其高表达与较差的总生存期相关[1]。在肝细胞癌中,细胞外囊泡来源的长链非编码RNA(lncRNA)THEMIS2-211被确定为一种肿瘤来源的循环生物标志物,其表达上调与肿瘤生长和转移相关[2]。

在TNBC中,Themis2的表达与癌症干细胞特性和化疗耐药性增强相关。Themis2通过抑制蛋白酪氨酸磷酸酶1B(PTP1B)与磷酸化MET(p-MET)和MET的结合,增强MET的激活,从而促进肿瘤干性、化疗耐药性和致瘤性[4]。此外,Themis2的表达与卵巢癌患者的预后相关,并且与上皮-间质转化(EMT)相关[5]。在心脏移植急性排斥反应(AR)的模型中,Themis2被确定为潜在的诊断基因生物标志物[6]。在类风湿性关节炎(RA)中,Themis2被确定为组织特异性表达的枢纽基因,具有诊断价值[7]。在宫颈癌的治疗中,Themis2被确定为潜在的治疗靶点[8]。此外,Themis2还在系统性红斑狼疮(SLE)和烟雾病(MMD)的基因交流中发挥作用[9]。

综上所述,Themis2在免疫细胞功能和多种癌症的发生发展中发挥重要作用。Themis2的表达上调与癌症患者的预后不良相关,且与免疫浸润、化疗耐药性和肿瘤干细胞特性增强相关。Themis2可能成为癌症诊断和治疗的新靶点。

参考文献:
1. Liu, Jun-Feng, Zou, Bing, Xiang, Cheng, Yan, Hai-Chao. 2024. Comprehensive bioinformatics analysis unveils THEMIS2 as a carcinogenic indicator related to immune infiltration and prognosis of thyroid cancer. In Scientific reports, 14, 8156. doi:10.1038/s41598-024-58943-6. https://pubmed.ncbi.nlm.nih.gov/38589421/
2. Yao, Jiyou, Hua, Xuefeng, Shi, Jiewei, Lan, Tian, Lu, Minqiang. . LncRNA THEMIS2-211, a tumor-originated circulating exosomal biomarker, promotes the growth and metastasis of hepatocellular carcinoma by functioning as a competing endogenous RNA. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36, e22238. doi:10.1096/fj.202101564R. https://pubmed.ncbi.nlm.nih.gov/35224785/
3. Hartweger, Harald, Schweighoffer, Edina, Davidson, Sophia, Wack, Andreas, Tybulewicz, Victor L J. 2014. Themis2 is not required for B cell development, activation, and antibody responses. In Journal of immunology (Baltimore, Md. : 1950), 193, 700-7. doi:10.4049/jimmunol.1400943. https://pubmed.ncbi.nlm.nih.gov/24907343/
4. Huang, Wei-Chieh, Yen, Jia-Hau, Sung, Yu-Wen, Huang, Shih-Jei, Wang, Lu-Hai. 2022. Novel function of THEMIS2 in the enhancement of cancer stemness and chemoresistance by releasing PTP1B from MET. In Oncogene, 41, 997-1010. doi:10.1038/s41388-021-02136-2. https://pubmed.ncbi.nlm.nih.gov/34974522/
5. Gao, Yang, Qi, Yuwen, Shen, Yin, Wang, Hua, Cai, Hongbing. 2024. Signatures of tumor-associated macrophages correlate with treatment response in ovarian cancer patients. In Aging, 16, 207-225. doi:10.18632/aging.205362. https://pubmed.ncbi.nlm.nih.gov/38175687/
6. Guo, Yiwen, Zhang, Yixi, Yu, Jia, Ju, Weiqiang, Chen, Maogen. 2023. Novel ceRNA network construction associated with programmed cell death in acute rejection of heart allograft in mice. In Frontiers in immunology, 14, 1184409. doi:10.3389/fimmu.2023.1184409. https://pubmed.ncbi.nlm.nih.gov/37753085/
7. Xing, Xuewu, Xia, Qun, Gong, Baoqi, Shen, Zhongyang, Zhang, Yingze. 2022. Identification of Tissue-Specific Expressed Hub Genes and Potential Drugs in Rheumatoid Arthritis Using Bioinformatics Analysis. In Frontiers in genetics, 13, 855557. doi:10.3389/fgene.2022.855557. https://pubmed.ncbi.nlm.nih.gov/35368701/
8. Zhang, Qiying, Zhang, Jing, Liu, Zi, Su, Jin, Zhao, Yalong. 2023. Recombinant Human Adenovirus Type 5 (H101) Intra-Tumor Therapy in Patients with Persistent, Recurrent, or Metastatic Cervical Cancer: Genomic Profiling Relating to Clinical Efficacy. In Drug design, development and therapy, 17, 3507-3522. doi:10.2147/DDDT.S429180. https://pubmed.ncbi.nlm.nih.gov/38046281/
9. Guo, Qingbao, Fan, Yan-Na, Xie, Manli, Bao, Xiangyang, Duan, Lian. 2024. Exploring the transcriptomic landscape of moyamoya disease and systemic lupus erythematosus: insights into crosstalk genes and immune relationships. In Frontiers in immunology, 15, 1456392. doi:10.3389/fimmu.2024.1456392. https://pubmed.ncbi.nlm.nih.gov/39290707/