Mylk4-KO 基因敲除小鼠

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产品名称

Mylk4-KO 基因敲除小鼠

产品编号

S-KO-15884

品系全称

C57BL/6JCya-Mylk4em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-238564-Mylk4-B6J-VA

品系状态

使用本品系发表的文献需注明: Mylk4-KO 基因敲除小鼠 mice (Strain S-KO-15884) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
myosin light chain kinase family, member 4
基因别称
EG238564
染色体号
Chr 13 (Mouse)
转录本 ID
NCBI: NM_001368880 | Ensembl: ENSMUST00000238977
修饰方式
全身性基因敲除
靶向范围
Exon 3~4
敲除长度
~1.7 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:3643758Homozygous null mice show slightly decreased body weight and impaired muscle strength with decreased isometric torque and passive stiffness.
MYLK4,也称为Myosin light-chain kinase 4,是肌球蛋白轻链激酶家族的一员。肌球蛋白轻链激酶家族是一类具有丝氨酸/苏氨酸特异性的酶,参与调节细胞内肌动蛋白和肌球蛋白的相互作用,进而影响细胞收缩和运动。MYLK4在肌肉发育、生长和力量维持等方面发挥重要作用[6]。

MYLK4基因的变异与多种生物学过程相关。研究表明,MYLK4基因的SNP与不同年龄段安纳托利亚黑牛的生长性状相关[1]。此外,MYLK4基因的拷贝数变异(CNV)对山羊的体重、体长和体高有显著影响,CNV的缺失类型与更好的生长性状相关[2]。在中国黄牛中,MYLK4基因的SNP与牛的生长性状相关,GG基因型与胸宽和臀宽显著相关[3]。

MYLK4基因的表观遗传修饰也与疾病相关。研究表明,沙丁醇治疗哮喘相关气道上皮细胞时,可以引起MYLK4基因的DNA甲基化水平变化,影响基因表达和生物学通路[4]。在结直肠癌细胞中,MYLK4基因呈现单等位基因表达,表明其在结直肠癌的发生发展中可能发挥重要作用[5]。

此外,MYLK4基因还与心脏疾病相关。研究表明,在心力衰竭患者的心肌细胞中,MYLK4基因表达下调,影响心肌细胞的收缩和细胞结构[7]。MYLK4基因的SNP与牛肉纹理和嫩度相关,表明其可能参与调节牛肉品质[8]。在鸡的肌肉发育过程中,MYLK4基因表达上调,参与调节肌肉生长和发育[9]。在乳腺癌中,MYLK4基因的SNP与ER-乳腺癌相关,表明其可能参与调节乳腺癌的发生发展[10]。

综上所述,MYLK4基因在肌肉发育、生长、力量维持、心脏疾病和癌症等方面发挥重要作用。MYLK4基因的变异和表观遗传修饰与多种生物学过程相关,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Aytekin, İbrahim, Bayraktar, Mervan, Sakar, Çağrı Melikşah, Ünal, İlker. 2020. Association between MYLK4 gene polymorphism and growth traits at different age stages in Anatolian black cattle. In Animal biotechnology, 31, 555-560. doi:10.1080/10495398.2020.1823402. https://pubmed.ncbi.nlm.nih.gov/32967525/
2. Shi, Shu-Yue, Li, Li-Juan, Zhang, Zi-Jing, Chen, Hong, Huang, Yong-Zhen. 2019. Copy number variation of MYLK4 gene and its growth traits of Capra hircus (goat). In Animal biotechnology, 31, 532-537. doi:10.1080/10495398.2019.1635137. https://pubmed.ncbi.nlm.nih.gov/31280665/
3. Zheng, Li, Zhang, Gui-Min, Dong, Yan-Peng, Huo, Li-Jun, Huang, Yong-Zhen. 2018. Genetic Variant of MYLK4 Gene and its Association with Growth Traits in Chinese Cattle. In Animal biotechnology, 30, 30-35. doi:10.1080/10495398.2018.1426594. https://pubmed.ncbi.nlm.nih.gov/29540101/
4. Perez-Garcia, Javier, Pino-Yanes, Maria, Plender, Elizabeth G, Seibold, Max A, Burchard, Esteban G. 2023. Epigenomic response to albuterol treatment in asthma-relevant airway epithelial cells. In Clinical epigenetics, 15, 156. doi:10.1186/s13148-023-01571-0. https://pubmed.ncbi.nlm.nih.gov/37784136/
5. Lee, Robin Dong-Woo, Song, Min-Young, Lee, Jong-Keuk. 2012. Large-scale profiling and identification of potential regulatory mechanisms for allelic gene expression in colorectal cancer cells. In Gene, 512, 16-22. doi:10.1016/j.gene.2012.10.001. https://pubmed.ncbi.nlm.nih.gov/23064046/
6. Sakakibara, Iori, Yanagihara, Yuta, Himori, Koichi, Sawasaki, Tatsuya, Imai, Yuuki. 2021. Myofiber androgen receptor increases muscle strength mediated by a skeletal muscle splicing variant of Mylk4. In iScience, 24, 102303. doi:10.1016/j.isci.2021.102303. https://pubmed.ncbi.nlm.nih.gov/33870126/
7. Herrer, Isabel, Roselló-Lletí, Esther, Rivera, Miguel, Montero, José Anastasio, Portolés, Manuel. 2014. RNA-sequencing analysis reveals new alterations in cardiomyocyte cytoskeletal genes in patients with heart failure. In Laboratory investigation; a journal of technical methods and pathology, 94, 645-53. doi:10.1038/labinvest.2014.54. https://pubmed.ncbi.nlm.nih.gov/24709777/
8. Won, KyeongHye, Kim, Dohyun, Hwang, Inho, Lee, Hak-Kyo, Oh, Jae-Don. 2023. Genome-wide association studies on collagen contents trait for meat quality in Hanwoo. In Journal of animal science and technology, 65, 311-323. doi:10.5187/jast.2022.e110. https://pubmed.ncbi.nlm.nih.gov/37093907/
9. Yin, Xuemei, Wu, Yulin, Zhang, Shanshan, Zhang, Genxi, Wang, Jinyu. 2021. Transcriptomic profile of leg muscle during early growth and development in Haiyang yellow chicken. In Archives animal breeding, 64, 405-416. doi:10.5194/aab-64-405-2021. https://pubmed.ncbi.nlm.nih.gov/34584942/
10. Lin, Yilong, Wang, Songsong, Zhang, Yun, Zhang, Liyi, Yang, Qingmo. 2024. Drug repurposing opportunities for breast cancer and seven common subtypes. In The Journal of steroid biochemistry and molecular biology, 246, 106652. doi:10.1016/j.jsbmb.2024.106652. https://pubmed.ncbi.nlm.nih.gov/39622444/